About: Further Characterisation of the Translational Termination-Reinitiation Signal of the Influenza B Virus Segment 7 RNA

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About: Further Characterisation of the Translational Termination-Reinitiation Signal of the Influenza B Virus Segment 7 RNA Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Further Characterisation of the Translational Termination-Reinitiation Signal of the Influenza B Virus Segment 7 RNA
Creator	Brown, Tdk Jackson, R Leigh, K Powell, M Pö Yry, Taa
Source	PMC
abstract	Termination-dependent reinitiation is used to co-ordinately regulate expression of the M1 and BM2 open-reading frames (ORFs) of the dicistronic influenza B segment 7 RNA. The start codon of the BM2 ORF overlaps the stop codon of the M1 ORF in the pentanucleotide UAA UG and ∼10% of ribosomes terminating at the M1 stop codon reinitiate translation at the overlapping AUG. BM2 synthesis requires the presence of, and translation through, 45 nt of RNA immediately upstream of the UAA UG, known as the ‘termination upstream ribosome binding site’ (TURBS). This region may tether ribosomal 40S subunits to the mRNA following termination and a short region of the TURBS, motif 1, with complementarity to helix 26 of 18S rRNA has been implicated in this process. Here, we provide further evidence for a direct interaction between mRNA and rRNA using antisense oligonucleotide targeting and functional analysis in yeast cells. The TURBS also binds initiation factor eIF3 and we show here that this protein stimulates reinitiation from both wild-type and defective TURBS when added exogenously, perhaps by stabilising ribosome-mRNA interactions. Further, we show that the position of the TURBS with respect to the UAA UG overlap is crucial, and that termination too far downstream of the 18S complementary sequence inhibits the process, probably due to reduced 40S tethering. However, in reporter mRNAs where the restart codon alone is moved downstream, termination-reinitiation is inhibited but not abolished, thus the site of reinitiation is somewhat flexible. Reinitiation on distant AUGs is not inhibited in eIF4G-depleted RRL, suggesting that the tethered 40S subunit can move some distance without a requirement for linear scanning.
has issue date	2011-02-08(xsd:dateTime)
bibo:doi	10.1371/journal.pone.0016822
bibo:pmid	21347434
has license	cc-by
sha1sum (hex)	9e0f14131900d5136cabf11f654a5cbb9d88ad48
schema:url	https://doi.org/10.1371/journal.pone.0016822
resource representing a document's title	Further Characterisation of the Translational Termination-Reinitiation Signal of the Influenza B Virus Segment 7 RNA
has PubMed Central identifier	PMC3035654
has PubMed identifier	21347434
schema:publication	PLoS One
resource representing a document's body	covid:9e0f14131900d5136cabf11f654a5cbb9d88ad48#body_text
is schema:about of	named entity 'ADDED' named entity 'WILD-TYPE' named entity 'ANTISENSE OLIGONUCLEOTIDE' named entity 'MOVE' named entity 'PRESENCE OF' named entity '18S RRNA' named entity 'TARGETING' named entity 'INTERACTIONS' named entity 'PROTEIN ' named entity 'TETHERED' named entity 'STOP CODON' named entity 'DISTANCE' named entity 'DEFECTIVE' named entity 'SEQUENCE' named entity 'YEAST CELLS' named entity 'SYNTHESIS' named entity 'ABOLISHED' named entity 'SOMEWHAT' named entity 'DUE TO' named entity 'INFLUENZA B' named entity 'HELIX' named entity 'BM2' named entity 'FLEXIBLE' named entity 'READING FRAMES' named entity 'SITE OF' named entity 'EIF4G' named entity 'START CODON' named entity 'IMMEDIATELY' named entity 'AUG.' named entity 'OVERLAPPING' named entity 'HERE' named entity 'DOWNSTREAM' named entity 'MOVED ' named entity 'restart' named entity 'ribosomal' named entity 'move' named entity '18S rRNA' named entity 'codon' named entity 'reduced' named entity 'segment' named entity 'rRNA' named entity 'UPSTREAM' named entity 'PROBABLY' named entity 'INHIBITED' named entity 'INITIATION FACTOR' named entity 'REDUCED' named entity '40S' named entity 'FUNCTIONAL ANALYSIS' named entity 'RIBOSOME BINDING SITE' named entity 'OPEN' named entity 'RIBOSOMAL' named entity 'BUT' named entity 'RRL' named entity 'REPORTER' named entity 'RRNA' named entity 'KNOWN' named entity 'EVIDENCE FOR' named entity 'DEPLETED' named entity 'COMPLEMENTARITY' named entity '18S' named entity 'RNA' named entity 'TRANSLATIONAL TERMINATION' named entity 'SIGNAL' named entity 'SEGMENT' named entity 'REGULATE' named entity 'TRANSLATION' named entity 'BINDS' named entity 'RIBOSOME' named entity 'SHORT' named entity 'POSITION' named entity 'ORF' named entity 'TETHER' named entity 'USING' named entity 'WHERE' named entity 'SEGMENT' named entity 'TERMINATION' named entity 'OVERLAPS'

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