About: Validation of Reference Genes for Gene Expression Studies in Aphis glycines (Hemiptera: Aphididae)

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About: Validation of Reference Genes for Gene Expression Studies in Aphis glycines (Hemiptera: Aphididae) Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Validation of Reference Genes for Gene Expression Studies in Aphis glycines (Hemiptera: Aphididae)
Creator	Bansal, Raman Mamidala, Praveen Michel, Andy Mittapalli, Omprakash Rouf Mian, M
Source	Medline; PMC
abstract	Quantitative real-time polymerase chain reaction (qRT-PCR) is a common and robust tool for accurate quantification of mRNA transcripts. To normalize results, a housekeeping gene ([HKG], reference gene or endogenous control gene) is mandatory. Soybean aphid, Aphis glycines Matsumura (Hemiptera: Aphididae), is a significant soybean, Glycine max (L.) Merr., pest, yet gene expression and functional genomics studies are hindered by a lack of stable HKGs. We evaluated seven potential HKGs (SDFS, succinate dehydrogenase flavoprotein subunit; EF1a, elongation factor-1α; HEL, helicase; GAPDH, glyceraldehyde-3 phosphate dehydrogenase; RPS9, ribosomal protein S9; TBP, TATA-box binding protein; and UBQ, ubiquitin-conjugating protein) to determine the most efficient HKGs that have stable expression among tissues, developmental stages, and aphids fed on susceptible and host plant–resistant soybean. HKG stability was determined using GeNorm and NormFinder. Results from three different experimental conditions revealed high stability of TBP compared with the other HKGs profiled across the samples assayed. RPS9 showed stable expression among aphids on susceptible and resistant plants, whereas EF1a showed stable expression in tissues and developmental stages. Therefore, we recommend the TBP as a suitable HKG for efficient normalization among treatments, tissues, and developmental stages of A. glycines. In addition, RPS9 may be used for host-plant resistance experiments and EF1a could be considered for testing differential expression across tissues or developmental stages. These results will enable a more accurate and reliable normalization of qRT-PCR data in A. glycines.
has issue date	2012-08-01(xsd:dateTime)
bibo:doi	10.1603/ec12095
bibo:pmid	22928326
has license	no-cc
sha1sum (hex)	9e1636fb626b72f4ba9eff80a9cec3b97f8de8c6
schema:url	https://doi.org/10.1603/ec12095
resource representing a document's title	Validation of Reference Genes for Gene Expression Studies in Aphis glycines (Hemiptera: Aphididae)
has PubMed Central identifier	PMC7110211
has PubMed identifier	22928326
schema:publication	J Econ Entomol
resource representing a document's body	covid:9e1636fb626b72f4ba9eff80a9cec3b97f8de8c6#body_text
is schema:about of	named entity 'Aphididae' named entity 'host' named entity 'functional genomics' named entity 'Matsumura' named entity 'TISSUES' named entity 'SUBUNIT' named entity 'DETERMINE' named entity 'DEHYDROGENASE' named entity 'GAPDH' named entity 'UBIQUITIN' named entity 'HKG' named entity 'FED' named entity 'STUDIES' named entity 'HIGH' named entity 'MRNA' named entity 'TOOL' named entity 'GENE EXPRESSION' named entity '2C HELICASE' named entity 'PEST' named entity 'EXPERIMENTAL CONDITIONS' named entity 'GENE' named entity 'DIFFERENT' named entity 'IS A' named entity 'LACK' named entity 'EF1A' named entity 'COMPARED' named entity 'EXPERIMENTS' named entity 'CONSIDERED' named entity 'HOUSEKEEPING GENE' named entity 'MAY BE' named entity 'USING' named entity 'ENDOGENOUS' named entity 'RESULTS' named entity 'PCR DATA' named entity 'FUNCTIONAL GENOMICS' named entity 'UBQ' named entity 'PROTEIN' named entity 'SUITABLE' named entity 'TESTING' named entity 'USED' named entity 'RESISTANCE' named entity 'HOST' named entity 'APHIDS' named entity 'PCR' named entity '90%' named entity 'SAMPLES' named entity 'HAVE' named entity 'STABLE' named entity 'GLYCINE MAX' named entity 'PLANT' named entity 'SOYBEAN APHID' named entity 'STUDIES' named entity 'TATA-BOX BINDING PROTEIN' named entity 'PLANTS' named entity 'DIFFERENTIAL EXPRESSION' named entity 'QRT-PCR' named entity 'APHIS GLYCINES' named entity 'RIBOSOMAL PROTEIN S9' named entity 'REFERENCE GENE' named entity 'PRO' named entity 'GLYCERALDEHYDE' named entity 'APHIS GLYCINES' named entity 'APHIDIDAE' named entity 'REFERENCE' named entity 'VALIDATION' named entity 'DEVELOPMENTAL STAGES' named entity 'CONTROL' named entity 'TREATMENTS' named entity 'HOST PLANT' named entity 'MANDATORY' named entity 'QUANTITATIVE REAL-TIME POLYMERASE CHAIN REACTION' named entity 'GENE EXPRESSION' named entity 'ACCURATE' named entity 'THESE' named entity 'RESISTANT' named entity 'RPS9' named entity 'A COMMON'

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