About: A disposable, integrated loop-mediated isothermal amplification cassette with thermally actuated valves

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About: A disposable, integrated loop-mediated isothermal amplification cassette with thermally actuated valves Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	A disposable, integrated loop-mediated isothermal amplification cassette with thermally actuated valves
Creator	Liu, Changchun Mauk, Michael Bau, H Haim, •
Source	PMC
abstract	An inexpensive, disposable, integrated, polymer-based cassette for loop-mediated isothermal amplification (LAMP) of target nucleic acids was designed, fabricated, and tested. The LAMP chamber was equipped with single-use, thermally actuated valves made with a composite consisting of a mixture of PDMS and expandable microspheres. The effect of the composite composition on its expansion was investigated, and the valve’s performance was evaluated. In its closed state, the valve can hold pressures as high as 200 kPa without any significant leakage. Both the LAMP chamber and the valves were actuated with thin film heaters. The utility of the cassette was demonstrated by carrying out LAMP of Escherichia coli DNA target and reverse transcribed loop meditated isothermal amplification (RT-LAMP) of RNA targets. The amplicons were detected in real time with a portable, compact detector. The system was capable of detecting as few as 10 target molecules per sample in well under 1 h. The portable, integrated cassette system described here is particularly suited for applications at the point of care and in resource-poor countries, where funds and trained personnel are in short supply. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s10404-011-0788-3) contains supplementary material, which is available to authorized users.
has issue date	2011-03-18(xsd:dateTime)
bibo:doi	10.1007/s10404-011-0788-3
bibo:pmid	32214952
has license	no-cc
sha1sum (hex)	9f5368c8a7d26569688cd4c6dcfb7e75f09865b1
schema:url	https://doi.org/10.1007/s10404-011-0788-3
resource representing a document's title	A disposable, integrated loop-mediated isothermal amplification cassette with thermally actuated valves
has PubMed Central identifier	PMC7088409
has PubMed identifier	32214952
schema:publication	Microfluid Nanofluidics
resource representing a document's body	covid:9f5368c8a7d26569688cd4c6dcfb7e75f09865b1#body_text
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