About: Brain Transcriptome-Wide Screen for HIV-1 Nef Protein Interaction Partners Reveals Various Membrane-Associated Proteins

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Brain Transcriptome-Wide Screen for HIV-1 Nef Protein Interaction Partners Reveals Various Membrane-Associated Proteins
Creator	Gorgels, A Hoffmann, Jonas Kammula, E Mö Tter, J
Source	PMC
abstract	HIV-1 Nef protein contributes essentially to the pathology of AIDS by a variety of protein-protein-interactions within the host cell. The versatile functionality of Nef is partially attributed to different conformational states and posttranslational modifications, such as myristoylation. Up to now, many interaction partners of Nef have been identified using classical yeast two-hybrid screens. Such screens rely on transcriptional activation of reporter genes in the nucleus to detect interactions. Thus, the identification of Nef interaction partners that are integral membrane proteins, membrane-associated proteins or other proteins that do not translocate into the nucleus is hampered. In the present study, a split-ubiquitin based yeast two-hybrid screen was used to identify novel membrane-localized interaction partners of Nef. More than 80% of the hereby identified interaction partners of Nef are transmembrane proteins. The identified hits are GPM6B, GPM6A, BAP31, TSPAN7, CYB5B, CD320/TCblR, VSIG4, PMEPA1, OCIAD1, ITGB1, CHN1, PH4, CLDN10, HSPA9, APR-3, PEBP1 and B3GNT, which are involved in diverse cellular processes like signaling, apoptosis, neurogenesis, cell adhesion and protein trafficking or quality control. For a subfraction of the hereby identified proteins we present data supporting their direct interaction with HIV-1 Nef. We discuss the results with respect to many phenotypes observed in HIV infected cells and patients. The identified Nef interaction partners may help to further elucidate the molecular basis of HIV-related diseases.
has issue date	2012-12-17(xsd:dateTime)
bibo:doi	10.1371/journal.pone.0051578
bibo:pmid	23284715
has license	cc-by
sha1sum (hex)	9f59f8a6813d0e450022c63b817e02f82e324b8c
schema:url	https://doi.org/10.1371/journal.pone.0051578
resource representing a document's title	Brain Transcriptome-Wide Screen for HIV-1 Nef Protein Interaction Partners Reveals Various Membrane-Associated Proteins
has PubMed Central identifier	PMC3524239
has PubMed identifier	23284715
schema:publication	PLoS One
resource representing a document's body	covid:9f59f8a6813d0e450022c63b817e02f82e324b8c#body_text
is schema:about of	named entity 'transmembrane proteins' named entity 'identified' named entity 'Interaction' named entity 'PROTEIN ' named entity 'PARTNERS' named entity 'PH4' named entity 'HITS' named entity 'PMEPA1' named entity 'STATES' named entity 'OBSERVED' named entity 'TO IDENTIFY' named entity 'Nef' named entity 'transcriptional activation' named entity 'membrane-associated proteins' named entity 'basis' named entity 'proteins' named entity 'cell adhesion' named entity 'genes' named entity 'identified' named entity 'HIV' named entity 'nucleus' named entity 'identified' named entity 'HIV-1' named entity 'HSPA9' named entity 'interaction partners' named entity 'integral membrane proteins' named entity 'myristoylation' named entity 'Nef protein' named entity 'PH4' named entity 'yeast two-hybrid screens' named entity 'cell adhesion' named entity 'interaction partners' named entity 'interaction partners' named entity 'GPM6B' named entity 'VSIG4' named entity 'CYB5B' named entity 'CHN1' named entity 'Wide Screen' named entity 'Transcriptome' named entity 'plasmid' named entity 'GPM6B' named entity 'vector' named entity 'host cell' named entity 'fluorescent protein' named entity 'protein' named entity 'vector' named entity 'interaction partners' named entity 'SV40 large T antigen' named entity 'antibody' named entity 'methanol' named entity 'GFP' named entity 'serotonin uptake' named entity 'lysosomes' named entity 'DSMZ' named entity 'plasmid' named entity 'refractory' named entity 'transcriptional activation' named entity 'HIV-1' named entity 'nucleus' named entity 'HIV-1' named entity 'GPM6B' named entity 'PBS' named entity 'MHC' named entity 'sense and antisense' named entity 'GPM6B' named entity 'cytoplasmic' named entity 'HIV-1' named entity 'gene transcription' named entity 'EXOC6' named entity 'false positive results' named entity 'b-galactosidase' named entity 'fluorescence' named entity 'protein' named entity 'HIV infection' named entity 'chemiluminescence' named entity 'cell lines' named entity 'cytoplasmic' named entity 'protein aggregation'

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