About: One-step real-time RT-PCR for pandemic influenza A virus (H1N1) 2009 matrix gene detection in swine samples

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About: One-step real-time RT-PCR for pandemic influenza A virus (H1N1) 2009 matrix gene detection in swine samples Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	One-step real-time RT-PCR for pandemic influenza A virus (H1N1) 2009 matrix gene detection in swine samples
Creator	Lorusso, Alessio Koster, Leo Faaberg, Kay Killian, Mary Vincent, Amy
Source	Elsevier; Medline; PMC
abstract	In the spring of 2009, a novel (H1N1) influenza A virus began to spread among humans worldwide. Although the 2009 H1N1 is related genetically to swine influenza viruses, human infection has not been connected to pig exposure. Because the virus is now circulating widely in the human population, swine herds are at increased risk of becoming infected. In order to investigate potential outbreaks of the 2009 pandemic virus in pigs, a quantitative real-time reverse transcriptase-polymerase chain reaction (qRT-PCR) for the detection of the (H1N1) 2009 RNA in clinical specimens was developed. To evaluate the applicability of the test as a diagnostic tool in the screening of field specimens from swine, 64 field isolates of North American swine, 5 equine and 48 avian influenza viruses collected during diagnostic investigations were analyzed retrospectively as well as samples collected during an experimental in vivo infection with two novel H1N1 isolates, A/California/04/2009 (H1N1)v virus and A/Mexico/4108/2009 (H1N1)v. The sensitivity of the qRT-PCR was shown to be higher with respect to standard techniques such as virus isolation and the reproducibility was satisfactory. The present unique and highly sensitive assay is able to detect as little as 1 × 10(1) copies of RNA per μl of template and it represents a rapid and useful approach for the screening and quantitation of (H1N1) 2009 RNA in porcine specimens.
has issue date	2009-12-17(xsd:dateTime)
bibo:doi	10.1016/j.jviromet.2009.12.002
bibo:pmid	20005904
has license	no-cc
sha1sum (hex)	9f6e9801833a46fab7cf5bb97683baff0c19b518
schema:url	https://doi.org/10.1016/j.jviromet.2009.12.002
resource representing a document's title	One-step real-time RT-PCR for pandemic influenza A virus (H1N1) 2009 matrix gene detection in swine samples
has PubMed Central identifier	PMC7119756
has PubMed identifier	20005904
schema:publication	J Virol Methods
resource representing a document's body	covid:9f6e9801833a46fab7cf5bb97683baff0c19b518#body_text
is schema:about of	named entity 'virus isolation' named entity 'virus' named entity 'swine' named entity 'H1N1' named entity 'viruses' named entity 'influenza A virus' named entity 'H1N1' named entity 'TO DETECT' named entity 'RELATED' named entity 'HUMAN POPULATION' named entity 'DIAGNOSTIC' named entity 'SPRING' named entity 'POTENTIAL' named entity 'INFECTION' named entity 'HUMAN' named entity 'TO INVESTIGATE' named entity 'REVERSE TRANSCRIPTASE-POLYMERASE CHAIN REACTION' named entity 'HIGHER' named entity 'PRESENT' named entity 'FIELD' named entity 'DIAGNOSTIC TOOL' named entity 'ASSAY' named entity 'NORTH AMERICAN' named entity 'STEP' named entity 'GENE' named entity 'INFECTED' named entity 'OUTBREAKS' named entity 'PIG' named entity 'PANDEMIC' named entity 'SCREENING' named entity 'QUANTITATION' named entity 'COLLECTED' named entity 'MATRIX' named entity 'PANDEMIC' named entity 'DETECTION' named entity 'INFLUENZA A VIRUS' named entity 'SAMPLES' named entity 'SWINE' named entity 'REAL-TIME RT-PCR' named entity 'SAMPLES' named entity 'EXPOSURE' named entity 'APPROACH' named entity 'TECHNIQUES' named entity 'ORDER' named entity 'VIRUS' named entity 'COPIES' named entity 'EQUINE' named entity 'INVESTIGATIONS' named entity 'RISK OF' named entity 'INCREASED RISK' named entity 'AS VIRUS' named entity 'SPREAD' named entity 'RESPECT' named entity 'ANALYZED' named entity 'EXPERIMENTAL' named entity 'HIGHLY SENSITIVE' named entity 'SWINE' named entity 'STANDARD' named entity 'NOVEL' named entity 'UNIQUE' named entity 'DETECTION' named entity 'EVALUATE' named entity 'SATISFACTORY' named entity 'TEST' named entity 'VIRUS ISOLATION' named entity 'CIRCULATING' named entity 'SWINE INFLUENZA' named entity 'RNA' named entity 'HUMANS' named entity 'REAL-TIME' named entity 'PIGS' named entity 'PCR' named entity 'LITTLE' named entity 'QUANTITATIVE' named entity 'WORLDWIDE' named entity 'TEMPLATE' named entity 'PORCINE'

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