About: Rapid identification of inhibitors that interfere with poliovirus replication using a cell-based assay

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Rapid identification of inhibitors that interfere with poliovirus replication using a cell-based assay
Creator	Chen, Wilfred Hwang, Yu-Chen Chu, Jang-Hann Yang, Priscilla Yates, Marylynn
source	Elsevier; Medline; PMC
abstract	Abstract A small molecule library containing 480 known bioactive compounds was screened for antiviral activity against poliovirus (PV) using a cellular fluorescence resonance energy transfer (FRET) assay for viral protease activity. The infected reporter cells treated with the viral replication-suppressing compounds were examined via fluorescence microscope 7.5h postinfection. Twelve molecules showed moderate to potent antiviral activity at concentrations less than 32μM during the primary screening. Three compounds, anisomycin, linoleic acid, and lycorine, were chosen for validation. A dose-dependent cytotoxicity assay and a secondary screening using conventional plaque assay were conducted to confirm the results. The developed method can be used for rapid screening for molecules with antiviral activity.
has issue date	2008-03-31(xsd:dateTime)
bibo:doi	10.1016/j.antiviral.2007.12.009
bibo:pmid	18243348
has license	els-covid
sha1sum (hex)	9fd538c6cbb1403b50d01d008c4f6a23e3e3c275
schema:url	https://doi.org/10.1016/j.antiviral.2007.12.009
resource representing a document's title	Rapid identification of inhibitors that interfere with poliovirus replication using a cell-based assay
has PubMed Central identifier	PMC7114228
has PubMed identifier	18243348
schema:publication	Antiviral Research
resource representing a document's body	covid:9fd538c6cbb1403b50d01d008c4f6a23e3e3c275#body_text
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