About: Detection of a novel avian influenza A (H7N9) virus in humans by multiplex one-step real-time RT-PCR assay

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About: Detection of a novel avian influenza A (H7N9) virus in humans by multiplex one-step real-time RT-PCR assay Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Detection of a novel avian influenza A (H7N9) virus in humans by multiplex one-step real-time RT-PCR assay
Creator	Chen, Yu Yu, Fei Cui, David Dong, Yuejiao Fan, Jian Guo, Xichao Huang, Xiaofeng Huo, Zhaoxia Lau, Siuying Li, Xuefen Lou, Jianzhou Tian, Li Xie, Guoliang Yang, Shigui Yang, Xianzhi Zheng, Shufa Zhu, Qiaoyun
Source	Medline; PMC
abstract	BACKGROUND: A novel avian influenza A (H7N9) virus emerged in eastern China in February 2013. 413 confirmed human cases, including 157 deaths, have been recorded as of July 31, 2014. METHODS: Clinical specimens, including throat swabs, sputum or tracheal aspirates, etc., were obtained from patients exhibiting influenza-like illness (ILIs), especially from those having pneumonia and a history of occupational exposure to poultry and wild birds. RNA was extracted from these samples and a multiplex one-step real-time RT-PCR assay was developed to specifically detect the influenza A virus (FluA). PCR primers targeted the conserved M and Rnase P (RP) genes, as well as the hemagglutinin and neuraminidase genes of the H7N9 virus. RESULTS: The multiplex assay specifically detected the avian H7N9 virus, and no cross-reaction with other common respiratory pathogens was observed. The detection limit of the assay was approximately 0.05 50% tissue culture infective doses (TCID(50)), or 100 copies per reaction. Positive detection of the H7N9 virus in sputum/tracheal aspirates was higher than in throat swabs during the surveillance of patients with ILIs. Additionally, detection of the matrix (M) and Rnase P genes aided in the determination of the novel avian H7N9 virus and ensured the quality of the clinical samples. CONCLUSIONS: These results demonstrate that the multiplex assay detected the novel avian H7N9 virus with high specificity and sensitivity, which is essential for the early diagnosis and treatment of infected patients. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/1471-2334-14-541) contains supplementary material, which is available to authorized users.
has issue date	2014-10-08(xsd:dateTime)
bibo:doi	10.1186/1471-2334-14-541
bibo:pmid	25298249
has license	cc-by
sha1sum (hex)	9fe4bb195ffbcf6f450478fa94e72e099fb7d335
schema:url	https://doi.org/10.1186/1471-2334-14-541
resource representing a document's title	Detection of a novel avian influenza A (H7N9) virus in humans by multiplex one-step real-time RT-PCR assay
has PubMed Central identifier	PMC4286936
has PubMed identifier	25298249
schema:publication	BMC Infect Dis
resource representing a document's body	covid:9fe4bb195ffbcf6f450478fa94e72e099fb7d335#body_text
is schema:about of	named entity 'VIRUS' named entity 'recorded' named entity 'genes' named entity 'H7N9' named entity 'RT-PCR' named entity 'avian influenza' named entity 'virus' named entity 'RT-PCR' covid:arg/9fe4bb195ffbcf6f450478fa94e72e099fb7d335 named entity 'GENES' named entity 'INCLUDING' named entity 'HUMAN' named entity 'HISTORY OF' named entity 'AVIAN INFLUENZA A' named entity 'HEMAGGLUTININ' named entity 'JULY' named entity 'FEBRUARY' named entity 'EXHIBITING' named entity 'TARGETED' named entity 'NOVEL' named entity 'EASTERN' named entity 'THESE' named entity 'ASPIRATES' named entity 'SPUTUM' named entity 'CASES' named entity 'DETECT' named entity 'VIRUS' named entity 'OCCUPATIONAL EXPOSURE' named entity 'POULTRY' named entity 'SAMPLES' named entity 'PCR PRIMERS' named entity 'MULTIPLEX' named entity 'PATIENTS' named entity 'THROAT' named entity 'DEATHS' named entity 'STEP' named entity 'CHINA' named entity 'INFLUENZA A VIRUS' named entity 'EXTRACTED' named entity 'BACKGROUND' named entity 'STEP' named entity 'MULTIPLEX' named entity 'HAVE' named entity 'OBTAINED' named entity 'INFLUENZA-LIKE ILLNESS' named entity 'H7N9' named entity 'SPECIMENS' named entity 'RT-PCR ASSAY' named entity 'NEURAMINIDASE' named entity 'RT-PCR ASSAY' named entity 'NOVEL' named entity 'HUMANS' named entity 'DETECTION' named entity 'AVIAN INFLUENZA A' named entity 'SWABS' named entity 'CONSERVED' named entity 'METHODS' named entity 'RNASE P' named entity 'REAL-TIME RT-PCR' named entity 'ETC.' named entity 'EXPOSURE TO' named entity 'RECORDED' named entity 'RNA' named entity 'CONFIRMED' named entity 'PNEUMONIA'

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