About: Development of polymerase chain reaction-based diagnostic tests for detection of Malsoor virus & adenovirus isolated from Rousettus species of bats in Maharashtra, India

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Development of polymerase chain reaction-based diagnostic tests for detection of Malsoor virus & adenovirus isolated from Rousettus species of bats in Maharashtra, India Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Development of polymerase chain reaction-based diagnostic tests for detection of Malsoor virus & adenovirus isolated from Rousettus species of bats in Maharashtra, India
Creator	Kokate, Prasad Kumar, Vimal Mourya, Devendra Patil, Deepak Shete, Anita Yadav, Pragya Mehershahi, Kurosh Nikam, Tushar
source	Medline; PMC
abstract	BACKGROUND & OBJECTIVES: Bats are recognized as important reservoirs for emerging infectious disease and some unknown viral diseases. Two novel viruses, Malsoor virus (family Bunyaviridae, genus, Phlebovirus) and a novel adenovirus (AdV) (family, Adenoviridae genus, Mastadenovirus), were identified from Rousettus bats in the Maharashtra State of India. This study was done to develop and optimize real time reverse transcription - polymerase chain reaction (RT-PCR) assays for Malsoor virus and real time and nested PCR for adenovirus from Rousettus bats. METHODS: For rapid and accurate screening of Malsoor virus and adenovirus a nested polymerase chain reaction and TaqMan-based real-time PCR were developed. Highly conserved region of nucleoprotein gene of phleboviruses and polymerase gene sequence from the Indian bat AdV isolate polyprotein gene were selected respectively for diagnostic assay development of Malsoor virus and AdV. Sensitivity and specificity of assays were calculated and optimized assays were used to screen bat samples. RESULTS: Molecular diagnostic assays were developed for screening of Malsoor virus and AdV and those were found to be specific. Based on the experiments performed with different parameters, nested PCR was found to be more sensitive than real-time PCR; however, for rapid screening, real-time PCR can be used and further nested PCR can be used for final confirmation or in those laboratories where real-time facility/expertise is not existing. INTERPRETATION & CONCLUSIONS: This study reports the development and optimization of nested RT-PCR and a TaqMan-based real-time PCR for Malsoor virus and AdV. The diagnostic assays can be used for rapid detection of these novel viruses to understand their prevalence among bat population.
has issue date	2017-01-01(xsd:dateTime)
bibo:doi	10.4103/ijmr.ijmr_1447_15
bibo:pmid	28574020
has license	cc-by-nc-sa
schema:url	https://doi.org/10.4103/ijmr.ijmr_1447_15
resource representing a document's title	Development of polymerase chain reaction-based diagnostic tests for detection of Malsoor virus & adenovirus isolated from Rousettus species of bats in Maharashtra, India
has PubMed Central identifier	PMC5460580
has PubMed identifier	28574020
schema:publication	Indian J Med Res
resource representing a document's body	covid:PMC5460580#body_text
is schema:about of	named entity 'species' named entity 'detection' named entity 'adenovirus' named entity 'Life Technologies' named entity 'Rousettus' named entity 'National Centre for Cell Science' named entity 'Uganda' named entity 'viral RNA' named entity 'real-time RT-PCR' named entity 'fever' named entity 'adenovirus' named entity 'nested PCR' named entity 'virus' named entity 'Mastadenovirus' named entity 'NCBI' named entity 'PCR' named entity 'adenovirus' named entity 'gene' named entity 'virus' named entity 'frugivorous' named entity 'PCR' named entity 'qRT-PCR' named entity 'virus' named entity 'Oligonucleotide' named entity 'primers' named entity 'virus' named entity 'virus' named entity 'TaqMan' named entity 'gene' named entity 'chikungunya' named entity 'polymerase' named entity 'real-time RT-PCR' named entity 'AdV' named entity 'RT-PCR' named entity 'virus' named entity 'adenoviral' named entity 'primers' named entity 'real-time PCR' named entity 'cloned DNA' named entity 'homology' named entity 'Ingwavuma' named entity 'adenovirus' named entity 'Carlsbad, CA' named entity 'AdV' named entity 'viral RNA' named entity 'AdV' named entity 'virus' named entity 'RNA' named entity 'AdV' named entity 'polymerase' named entity 'embryo' named entity 'Maharashtra State' named entity 'AdV' named entity 'PCR' named entity 'protein' named entity 'tissue culture' named entity 'secondary structure' named entity 'NIV' named entity 'AdV' named entity 'adenovirus' named entity 'PCR' named entity 'primers' named entity 'Pteropus' named entity 'gene' named entity 'NCBI' named entity 'primers' named entity 'polymerase' named entity 'G+C' named entity 'AdV' named entity 'RNA' named entity 'qRT-PCR' named entity 'complementarity' named entity 'virus' named entity 'nucleoprotein' named entity 'S-segment'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2025 OpenLink Software