About: Evaluation of real-time reverse-transcription loop-mediated isothermal amplification assay for clinical diagnosis of West Nile virus in patients

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About: Evaluation of real-time reverse-transcription loop-mediated isothermal amplification assay for clinical diagnosis of West Nile virus in patients Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Evaluation of real-time reverse-transcription loop-mediated isothermal amplification assay for clinical diagnosis of West Nile virus in patients
Creator	Parida, Manmohan Rathinam, Sivakumar Saxena, Divyasha Kumar, Jyoti
source	Medline; PMC
abstract	BACKGROUND & OBJECTIVES: West Nile virus (WNV) is a mosquito-borne flavivirus. The disease can be diagnosed by isolation followed by fluorescent antibody tests, enzyme-linked immunosorbent assay and polymerase chain reaction (PCR) assay. These diagnostic methods are laborious and time-consuming. The present study was aimed to evaluate the real-time reverse-transcription loop-mediated isothermal amplification (RT-LAMP) method for rapid, early and accurate diagnosis of WNV. METHODS: A one-step single tube accelerated quantitative RT-LAMP assay was evaluated by targeting the Env gene of WNV. The gene amplification was accomplished by incubating the reaction mixture at 63°C for 60 min in both real time turbidimeter as well as routine laboratory water bath/dry heating bath. To rule out contamination issues, proper negative controls, including no template, no primer; and no enzyme, were always kept alongside each run. The RT-LAMP assay was evaluated on 105 clinical samples from individuals having ocular infection. RESULTS: Of the 105 samples tested, 27 were positive for WNV by RT-LAMP assay. The comparative evaluation with conventional RT-PCR revealed 100 per cent accordance with sensitivity and specificity of 100 and 95 per cent, respectively. The specificity of this assay was confirmed with serum samples obtained from patients with dengue and chikungunya. INTERPRETATION & CONCLUSIONS: The RT-LAMP test seemed to be a sensitive and specific method for rapid detection of WNV infection and would be useful for rapid screening of a large number of clinical samples in endemic areas during outbreaks.
has issue date	2018-03-01(xsd:dateTime)
bibo:doi	10.4103/0971-5916.234607
bibo:pmid	29923519
has license	cc-by-nc-sa
schema:url	https://doi.org/10.4103/0971-5916.234607
resource representing a document's title	Evaluation of real-time reverse-transcription loop-mediated isothermal amplification assay for clinical diagnosis of West Nile virus in patients
has PubMed Central identifier	PMC6022379
has PubMed identifier	29923519
schema:publication	Indian J Med Res
resource representing a document's body	covid:PMC6022379#body_text
is schema:about of	named entity 'real-time' named entity 'West Nile' named entity 'developing countries' named entity 'pmol' named entity 'turbidity' named entity 'study protocol' named entity 'RT-PCR' named entity 'RT-PCR' named entity 'RT-PCR' named entity 'turbidity' named entity 'pmol' named entity 'nuclease' named entity 'clinical diagnosis' named entity 'cross-reactivity' named entity 'TaqMan' named entity 'RNA' named entity 'RNA' named entity 'amplicon' named entity 'serum' named entity 'fluorogenic' named entity 'precipitate' named entity 'optical density' named entity 'CSF' named entity 'DNA polymerase' named entity 'negative predictive values' named entity 'negative control' named entity 'Madurai' named entity 'PCR' named entity 'chikungunya' named entity 'dye' named entity 'turbidity' named entity 'RT-PCR' named entity 'LA-200' named entity 'Madurai' named entity 'turbidity' named entity 'magnesium' named entity 'India' named entity 'sensitivity and specificity' named entity 'plasma' named entity '0.01' named entity 'genome' named entity 'brain tissues' named entity 'Japan' named entity 'WNV' named entity 'primers' named entity 'genome' named entity 'real-time RT-PCR' named entity 'Qiagen' named entity 'WNV' named entity 'serum samples' named entity 'pmol' named entity 'Eiken Chemical' named entity 'India' named entity 'Aravind Eye Hospital' named entity 'turbidity' named entity 'urine' named entity 'viral disease' named entity 'gene sequences' named entity 'primers' named entity 'kinetics' named entity 'RNA' named entity 'complement' named entity 'primers' named entity 'PCR-based methods' named entity 'dye' named entity 'plaque-forming unit' named entity 'TGG' named entity 'Australia' named entity 'virus' named entity 'human beings' named entity 'Promega' named entity 'SYBR Green' named entity 'RNA extraction' named entity 'Japan' named entity 'WNV' named entity 'diagnostic tool' named entity 'supernatant' named entity 'Turbidimeter' named entity 'chikungunya'

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