About: Study of interactions between actinomycin D and oligonucleotides by microchip electrophoresis and ESI-MS

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Study of interactions between actinomycin D and oligonucleotides by microchip electrophoresis and ESI-MS
Creator	Lin, Bingcheng Zhou, Xiaomian He, Xinya Li, Dazhi Shen, Zheng
Source	Elsevier; Medline; PMC
abstract	In the present study, the interactions between actinomycin D (ActD) and single stranded DNA (ssDNA) 5′-CGTAACCAACTGCAACGT-3′ and a duplex stranded DNA (dsDNA) with this sequence were investigated by microchip-based non-gel sieving electrophoresis and electrospray ionization mass spectrometry (ESI-MS). The ssDNA was designed according to the conserved regions of open reading frame 1b (replicase 1B) following the Tor 2 SARS genome sequence of 15611-15593. The binding constants of the interactions between ActD and ssDNA/dsDNA were (8.3 ± 0.32) × 10(6) M(−1) (ssDNA) and (2.8 ± 0.02) × 10(5) M(−1) (dsDNA), respectively, calculated from microchip electrophoresis via Scatchard plot. The binding stoichiometries were 1:1 (single/1ActD molecule) and 1:2 (duplex/2ActD molecules) calculated from microchip electrophoresis, and the results were further verified by ESI-MS. The results obtained by these two methods indicated that ActD bound much more tightly to ssDNA used in this work than dsDNA. Furthermore, this is shown that the microchip-based non-gel sieving electrophoresis method is a rapid, highly sensitive and convenient method for the studies of interactions between DNA and small molecule drugs.
has issue date	2007-04-30(xsd:dateTime)
bibo:doi	10.1016/j.talanta.2006.11.019
bibo:pmid	19071655
has license	no-cc
schema:url	https://doi.org/10.1016/j.talanta.2006.11.019
resource representing a document's title	Study of interactions between actinomycin D and oligonucleotides by microchip electrophoresis and ESI-MS
has PubMed Central identifier	PMC7111764
has PubMed identifier	19071655
schema:publication	Talanta
resource representing a document's body	covid:PMC7111764#body_text
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