About: Development and Evaluation of a One-Step Quantitative RT-PCR Assay for Detection of Lassa Virus

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Development and Evaluation of a One-Step Quantitative RT-PCR Assay for Detection of Lassa Virus
Creator	Ayginin, Andrey Dedkov, Vladimir Safonova, Marina Camara, Amara Camara, Jacob Kourouma, Fode Kritzkiy, Andrey Maleev, Victor Naydenova, Ekaterina Shchelkanov, Mikhail Soropogui, Barre Tuchkov, Igor Magassouba, N.’Faly
Source	PMC
abstract	Lassa fever is a severe viral hemorrhagic illness caused by Lassa virus. Based on estimates, the number of LASV infections ranges from 300,000 to 500,000 cases in endemic areas with a fatality rate of 1%. Development of fast and sensitive tools for the control and prevention of Lassa virus infection as well as for clinical diagnostics of Lassa fever are crucial. Here we reported development and evaluation of a one-step quantitative RT-qPCR assay for the Lassa virus detection – LASV-Fl. This assay is suitable for the detection of lineages I-IV of Lassa virus. The limit of detection of the assay ranged from 10(3) copies/ml to 10(5) copies/ml and has 96.4% diagnostic sensitivity, whereas analytical and diagnostic specificities both were 100%. Serum, whole blood and tissue are suitable for use with the assay. The assay contains all the necessary components to perform the analysis, including an armored positive control (ARC+) and an armored internal control (IC). The study was done during the mission of specialized anti-epidemic team of the Russian Federation (SAET) in the Republic of Guinea in 2015-2018. Based on sequencing data, LASV-specific assay was developed using synthetic MS2-phage-based armored RNA particles, RNA from Lassa virus strain Josiah, and further, evaluated in field conditions using samples from patients and Mastomys natalensis rodents.
has issue date	2019-06-03(xsd:dateTime)
bibo:doi	10.1016/j.jviromet.2019.113674
bibo:pmid	31170468
has license	no-cc
schema:url	https://doi.org/10.1016/j.jviromet.2019.113674
resource representing a document's title	Development and Evaluation of a One-Step Quantitative RT-PCR Assay for Detection of Lassa Virus
has PubMed Central identifier	PMC7113850
has PubMed identifier	31170468
schema:publication	J Virol Methods
resource representing a document's body	covid:PMC7113850#body_text
is schema:about of	named entity 'RT-PCR' named entity 'Lassa Virus' named entity 'RT-PCR' named entity 'LASV' named entity 'RNA sequence' named entity 'Qiagen' named entity 'LOD' named entity 'real-time polymerase chain reaction' named entity 'LASV' named entity 'viral species' named entity 'fluorescence' named entity 'Lassa fever' named entity 'plasmid' named entity 'LASV' named entity 'Russia' named entity 'West African' named entity 'Bio-Rad' named entity 'genus' named entity 'Fermentas' named entity 'Guinea' named entity 'RT-qPCR' named entity 'cross-contamination' named entity 'Kindia' named entity 'LOD' named entity 'Lassa fever' named entity 'Germany' named entity 'covalently' named entity 'RNA' named entity 'Biotechnology' named entity 'DNA sequencing' named entity 'nosocomial' named entity 'Virology' named entity 'plasmid vector' named entity 'LASV' named entity 'genomes' named entity 'logarithmic units' named entity 'abdominal pain' named entity 'LASV' named entity 'Virology' named entity 'Lassa' named entity 'Research Project' named entity '95% confidence interval' named entity 'Russia' named entity 'gene' named entity 'LASV' named entity 'fever' named entity 'black hole' named entity 'Qiagen' named entity 'Russia' named entity 'Lassa fever' named entity 'viral RNA' named entity 'cell culture' named entity 'endemic areas' named entity 'LASV' named entity 'Côte d'Ivoire' named entity 'RNA' named entity 'Russia' named entity 'fatality rate' named entity 'LOD' named entity 'lysed' named entity 'plasmids' named entity 'infection' named entity 'RNA extraction' named entity 'Bio-Rad' named entity 'ambisense' named entity 'virus strain' named entity 'recombinant' named entity 'reverse transcription' named entity 'primers' named entity 'conserved regions'

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