About: Multiplexed detection of influenza A virus subtype H5 and H9 via quantum dot-based immunoassay

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About: Multiplexed detection of influenza A virus subtype H5 and H9 via quantum dot-based immunoassay Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Multiplexed detection of influenza A virus subtype H5 and H9 via quantum dot-based immunoassay
Creator	Liu, Qian Ma, Lan Cen, Yu Mao, Mao Qin, Zhifeng Shen, Huaibin Song Li, Lin Wu, Feng Yuan, Hang Zhou, Changhua
source	Elsevier; Medline; PMC
abstract	A quantum dot-based lateral flow immunoassay system (QD-LFIAS) was developed to simultaneously detect both influenza A virus subtypes H5 and H9. Water-soluble carboxyl-functionalized quantum dots (QDs) were used as fluorescent tags. The QDs were conjugated to specific influenza A virus subtype H5 and H9 antibodies via an amide bond. When influenza A virus subtype H5 or H9 was added to the QD-LFIAS, the QD-labeled antibodies specifically bound to the H5 or H9 subtype viruses and were then captured by the coating antibodies at test line 1 or 2 to form a sandwich complex. This complex produced a bright fluorescent band in response to 365 nm ultraviolet excitation. The intensity of fluorescence can be detected using an inexpensive, low-maintenance instrument, and the virus concentration directly correlates with the fluorescence intensity. The detection limit of the QD-LFIAS for influenza A virus subtype H5 was 0.016 HAU, and the detection limit of the QD-LFIAS for influenza A virus subtype H9 was 0.25 HAU. The specificity and reproducibility were good. The simple analysis step and objective results that can be obtained within 15 min indicate that this QD-LFIAS is a highly efficient test that can be used to monitor and prevent both Influenza A virus subtypes H5 and H9.
has issue date	2016-03-15(xsd:dateTime)
bibo:doi	10.1016/j.bios.2015.10.002
bibo:pmid	26454828
has license	no-cc
schema:url	https://doi.org/10.1016/j.bios.2015.10.002
resource representing a document's title	Multiplexed detection of influenza A virus subtype H5 and H9 via quantum dot-based immunoassay
has PubMed Central identifier	PMC7126372
has PubMed identifier	26454828
schema:publication	Biosens Bioelectron
resource representing a document's body	covid:PMC7126372#body_text
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