About: Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in human serum using a localized surface plasmon coupled fluorescence fiber-optic biosensor

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About: Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in human serum using a localized surface plasmon coupled fluorescence fiber-optic biosensor Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in human serum using a localized surface plasmon coupled fluorescence fiber-optic biosensor
Creator	Chang, Ying-Feng Chen, Chii-Chang Chen, Kuan-Hsuan Chou, Chien Lee, Chun-Wei Su, Li-Chen Chen, Yi-Ming Huang, Jason
Source	Elsevier; Medline; PMC
abstract	In order to enhance the sensitivity of conventional immunoassay technology for the detection of SARS coronavirus (SARS-CoV) nucleocapsid protein (N protein), we developed a localized surface plasmon coupled fluorescence (LSPCF) fiber-optic biosensor that combines sandwich immunoassay with the LSP technique. Experimentally, a linear relationship between the fluorescence signal and the concentration of recombinant SARS-CoV N (GST-N) protein in buffer solution could be observed from 0.1 pg/mL to 1 ng/mL. In addition, the concentration of GST-N protein in diluted serum across a similar range could also be measured. The correlation coefficients (linear scale) for these two measurements were 0.9469 and 0.9624, respectively. In comparison with conventional enzyme linked immunosorbent assay (ELISA), the detection limit of the LSPCF fiber-optic biosensor for the GST-N protein was improved at least 10(4)-fold using the same monoclonal antibodies. Therefore, the LSPCF fiber-optic biosensor shows an ability to detect very low concentration (∼1 pg/mL) of SARS-CoV N protein in serum. The biosensor should help with the early diagnosis of SARS infection.
has issue date	2009-10-15(xsd:dateTime)
bibo:doi	10.1016/j.bios.2009.07.012
bibo:pmid	19660929
has license	no-cc
schema:url	https://doi.org/10.1016/j.bios.2009.07.012
resource representing a document's title	Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in human serum using a localized surface plasmon coupled fluorescence fiber-optic biosensor
has PubMed Central identifier	PMC7127111
has PubMed identifier	19660929
schema:publication	Biosens Bioelectron
resource representing a document's body	covid:PMC7127111#body_text
is schema:about of	named entity 'coronavirus' named entity 'Detection' named entity 'fiber-optic' named entity 'nucleocapsid protein' named entity 'coronavirus' named entity 'pg/mL' named entity 'nucleocapsid' named entity 'Tris base' named entity 'secondary antibodies' named entity 'nucleocapsid' named entity 'sandwich ELISA' named entity 'protein' named entity 'rolling circle amplification' named entity 'GST' named entity 'PCR' named entity 'light source' named entity 'clinical symptoms' named entity 'nucleocapsid' named entity 'cDNA' named entity 'pg/mL' named entity '2-propanol' named entity 'BSA' named entity 'adsorption' named entity 'nucleocapsid' named entity 'wavelength' named entity 'protein' named entity 'infection' named entity 'Tokyo' named entity 'ethyl acetate' named entity 'nucleocapsid' named entity 'IPTG' named entity 'fluorescence' named entity 'Bovine serum albumin' named entity 'murine' named entity 'hybridoma' named entity 'detection limit' named entity 'GST' named entity 'SDS' named entity 'antibody response' named entity 'recombinant' named entity 'St. Louis, MO' named entity 'nucleocapsid' named entity 'fiber-optic' named entity 'molar extinction coefficients' named entity 'nucleocapsid' named entity 'linear relationship' named entity 'GST' named entity 'GST' named entity '0.45' named entity 'protein' named entity 'protein' named entity 'Severe acute respiratory syndrome' named entity '11.1' named entity 'fluorescent labeling' named entity 'SARS-CoV' named entity 'SARS' named entity 'bacteria' named entity 'nucleocapsid' named entity 'Japan' named entity 'GST' named entity 'PBS' named entity 'protein' named entity 'PCR-based' named entity 'protein' named entity 'pg/mL' named entity 'GST' named entity 'fluorophore' named entity 'immunoglobulin' named entity 'polyclonal antibody' named entity 'biosensor' named entity 'GST' named entity 'GST' named entity 'GST' named entity 'Japan' named entity 'correlation coefficient'

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