About: Discovery of Aptamers Targeting the Receptor-Binding Domain of the SARS-CoV-2 Spike Glycoprotein

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Discovery of Aptamers Targeting the Receptor-Binding Domain of the SARS-CoV-2 Spike Glycoprotein
Creator	Song, Yanling Zhu, Zhi Huang, Mengjiao Song, Jia Yang, Chaoyong Zhu, Lin Lin, Bingqian Shen, Haicong Sun, Miao Wei, Xinyu
Source	Medline; PMC
abstract	[Image: see text] The World Health Organization has declared the outbreak of a novel coronavirus (SARS-CoV-2 or 2019-nCoV) as a global pandemic. However, the mechanisms behind the coronavirus infection are not yet fully understood, nor are there any targeted treatments or vaccines. In this study, we identified high-binding-affinity aptamers targeting SARS-CoV-2 RBD, using an ACE2 competition-based aptamer selection strategy and a machine learning screening algorithm. The K(d) values of the optimized CoV2-RBD-1C and CoV2-RBD-4C aptamers against RBD were 5.8 nM and 19.9 nM, respectively. Simulated interaction modeling, along with competitive experiments, suggests that two aptamers may have partially identical binding sites at ACE2 on SARS-CoV-2 RBD. These aptamers present an opportunity for generating new probes for recognition of SARS-CoV-2 and could provide assistance in the diagnosis and treatment of SARS-CoV-2 while providing a new tool for in-depth study of the mechanisms behind the coronavirus infection.
has issue date	2020-06-17(xsd:dateTime)
bibo:doi	10.1021/acs.analchem.0c01394
bibo:pmid	32551560
has license	no-cc
schema:url	https://doi.org/10.1021/acs.analchem.0c01394
resource representing a document's title	Discovery of Aptamers Targeting the Receptor-Binding Domain of the SARS-CoV-2 Spike Glycoprotein
has PubMed Central identifier	PMC7336720
has PubMed identifier	32551560
schema:publication	Anal Chem
resource representing a document's body	covid:PMC7336720#body_text
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