About: Detection of coronaviruses in Pteropus & Rousettus species of bats from different States of India

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About: Detection of coronaviruses in Pteropus & Rousettus species of bats from different States of India Goto Sponge NotDistinct Permalink

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Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Detection of coronaviruses in Pteropus & Rousettus species of bats from different States of India
Creator	Gupta, Nivedita Sudeep, A.B. Mourya, Devendra Yadav, Pragya Ullas, Padinjaremattathil Kumar, Abhimanyu Majumdar, Triparna Nyayanit, Dimpal Shete-Aich, Anita Balasubramanian, R. Baradkar, Sreekant Dighe, Hitesh Gokhale, M.D. Joshi, Yash Kharde, Rutuja Mathapati, Basavaraj Mohandas, Sreelekshmy Pardeshi, Prachi Patil, Dilip Patil, Savita Salve, Malvika Sawant, Pradeep
source	Medline; PMC
abstract	BACKGROUND & OBJECTIVES: Bats are considered to be the natural reservoir for many viruses, of which some are potential human pathogens. In India, an association of Pteropus medius bats with the Nipah virus was reported in the past. It is suspected that the recently emerged severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) also has its association with bats. To assess the presence of CoVs in bats, we performed identification and characterization of bat CoV (BtCoV) in P. medius and Rousettus species from representative States in India, collected during 2018 and 2019. METHODS: Representative rectal swab (RS) and throat swab specimens of Pteropus and Rousettus spp. bats were screened for CoVs using a pan-CoV reverse transcription-polymerase chain reaction (RT-PCR) targeting the RNA-dependent RNA polymerase (RdRp) gene. A single-step RT-PCR was performed on the RNA extracted from the bat specimens. Next-generation sequencing (NGS) was performed on a few representative bat specimens that were tested positive. Phylogenetic analysis was carried out on the partial sequences of RdRp gene sequences retrieved from both the bat species and complete viral genomes recovered from Rousettus spp. RESULTS: Bat samples from the seven States were screened, and the RS specimens of eight Rousettus spp. and 21 Pteropus spp. were found positive for CoV RdRp gene. Among these, by Sanger sequencing, partial RdRp sequences could be retrieved from three Rousettus and eight Pteropus bat specimens. Phylogenetic analysis of the partial RdRp region demonstrated distinct subclustering of the BtCoV sequences retrieved from these Rousettus and Pteropus spp. bats. NGS led to the recovery of four sequences covering approximately 94.3 per cent of the whole genome of the BtCoVs from Rousettus bats. Three BtCoV sequences had 93.69 per cent identity to CoV BtRt-BetaCoV/GX2018. The fourth BtCoV sequence was 96.8 per cent identical to BtCoV HKU9-1. INTERPRETATION & CONCLUSIONS: This study was a step towards understanding the CoV circulation in Indian bats. Detection of potentially pathogenic CoVs in Indian bats stresses the need for enhanced screening for novel viruses in them. One Health approach with collaborative activities by the animal health and human health sectors in these surveillance activities shall be of use to public health. This would help in the development of diagnostic assays for novel viruses with outbreak potential and be useful in disease interventions. Proactive surveillance remains crucial for identifying the emerging novel viruses with epidemic potential and measures for risk mitigation.
has issue date	2020-01-01(xsd:dateTime)
bibo:doi	10.4103/ijmr.ijmr_795_20
bibo:pmid	32317409
has license	cc-by-nc-sa
schema:url	https://doi.org/10.4103/ijmr.ijmr_795_20
resource representing a document's title	Detection of coronaviruses in Pteropus & Rousettus species of bats from different States of India
has PubMed Central identifier	PMC7366549
has PubMed identifier	32317409
schema:publication	Indian J Med Res
resource representing a document's body	covid:PMC7366549#body_text
is schema:about of	named entity 'bats' named entity 'Rousettus' named entity 'coronaviruses' named entity 'nucleocapsid' named entity 'Phylogenetic analysis' named entity 'epidemiological' named entity 'RdRp' named entity 'Rousettus' named entity 'Qiagen' named entity 'gel extraction' named entity 'Sanger' named entity 'SARS-CoV-1' named entity 'RdRp' named entity 'Nipah virus' named entity 'gene' named entity 'Rousettus' named entity 'Pteropus' named entity 'intermediate host' named entity 'genus' named entity 'RdRp' named entity 'Invitrogen' named entity 'library preparation' named entity 'SARS-CoV-2' named entity 'Coronavirinae' named entity 'genome sequences' named entity 'ORF' named entity 'common cold' named entity 'Thailand' named entity 'Kerala' named entity 'spin kit' named entity 'gene sequences' named entity 'β-CoV' named entity 'ligation' named entity 'β-CoV' named entity 'viruses' named entity 'Terminator 3' named entity 'Germany' named entity 'β-CoV' named entity 'New England Biolabs' named entity 'Roche' named entity 'Hilden' named entity 'RdRp' named entity 'viruses' named entity 'Illumina' named entity 'Western Ghats' named entity 'envelope protein' named entity 'GenBank' named entity 'Rousettus' named entity '5.8' named entity '5.1' named entity 'genus' named entity 'RdRp' named entity 'viruses' named entity 'Kerala' named entity 'whole-genome sequences' named entity 'Rousettus' named entity 'Illumina' named entity 'HCoV-OC43' named entity 'Tamil Nadu' named entity 'qiagen' named entity 'genome'

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