About: Detection of SARS-CoV-2 RNA in commercial passenger aircraft and cruise ship wastewater: a surveillance tool for assessing the presence of COVID-19 infected travellers

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Detection of SARS-CoV-2 RNA in commercial passenger aircraft and cruise ship wastewater: a surveillance tool for assessing the presence of COVID-19 infected travellers Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Detection of SARS-CoV-2 RNA in commercial passenger aircraft and cruise ship wastewater: a surveillance tool for assessing the presence of COVID-19 infected travellers
Creator	Kitajima, Masaaki Bibby, Kyle Ahmed, Warish Hamilton, Kerry Smith, Wendy Bivins, Aaron Symonds, Erin Angel, Nicola Bertsch, Paul Dierens, Leanne Edson, Janette Ehret, John Gyawali, Pradip Hosegood, Ian Hugenholtz, Philip Jiang, Guangming Mueller, Jochen Shi, Jiahua Shimko, Katja Sichani, Homa Simpson, Stuart Thomas, Kevin Verhagen, Rory Zaugg, Julian
Source	Medline; PMC
abstract	BACKGROUND: Wastewater-based epidemiology (WBE) for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can be an important source of information for coronavirus disease 2019 (COVID-19) management during and after the pandemic. Currently, governments and transportation industries around the world are developing strategies to minimize SARS-CoV-2 transmission associated with resuming activity. This study investigated the possible use of SARS-CoV-2 RNA wastewater surveillance from airline and cruise ship sanitation systems and its potential use as a COVID-19 public health management tool. METHODS: Aircraft and cruise ship wastewater samples (n = 21) were tested for SARS-CoV-2 using two virus concentration methods, adsorption–extraction by electronegative membrane (n = 13) and ultrafiltration by Amicon (n = 8), and five assays using reverse-transcription quantitative polymerase chain reaction (RT-qPCR) and RT-droplet digital PCR (RT-ddPCR). Representative qPCR amplicons from positive samples were sequenced to confirm assay specificity. RESULTS: SARS-CoV-2 RNA was detected in samples from both aircraft and cruise ship wastewater; however concentrations were near the assay limit of detection. The analysis of multiple replicate samples and use of multiple RT-qPCR and/or RT-ddPCR assays increased detection sensitivity and minimized false-negative results. Representative qPCR amplicons were confirmed for the correct PCR product by sequencing. However, differences in sensitivity were observed among molecular assays and concentration methods. CONCLUSIONS: The study indicates that surveillance of wastewater from large transport vessels with their own sanitation systems has potential as a complementary data source to prioritize clinical testing and contact tracing among disembarking passengers. Importantly, sampling methods and molecular assays must be further optimized to maximize detection sensitivity. The potential for false negatives by both wastewater testing and clinical swab testing suggests that the two strategies could be employed together to maximize the probability of detecting SARS-CoV-2 infections amongst passengers.
has issue date	2020-07-14(xsd:dateTime)
bibo:doi	10.1093/jtm/taaa116
bibo:pmid	32662867
has license	no-cc
schema:url	https://doi.org/10.1093/jtm/taaa116
resource representing a document's title	Detection of SARS-CoV-2 RNA in commercial passenger aircraft and cruise ship wastewater: a surveillance tool for assessing the presence of COVID-19 infected travellers
has PubMed Central identifier	PMC7454825
has PubMed identifier	32662867
schema:publication	J Travel Med
resource representing a document's body	covid:PMC7454825#body_text
is schema:about of	named entity 'PRESENCE OF' named entity 'COMMERCIAL' named entity 'TOOL' named entity 'WASTEWATER' named entity 'PASSENGER' named entity 'aircraft' named entity 'cruise ship' named entity 'DNase' named entity 'epidemics' named entity 'hexylene glycol' named entity 'Bio-Rad Laboratories' named entity 'RT-qPCR' named entity 'infection status' named entity 'gene' named entity 'Australia' named entity 'RNA' named entity 'viral RNA' named entity 'passenger aircraft' named entity 'SARS-CoV-2' named entity 'false-negative' named entity 'SARS-CoV-2' named entity 'infectivity' named entity 'clinical surveillance' named entity 'RNA' named entity 'SARS-CoV-2' named entity 'Bio-Rad' named entity 'SARS-CoV-2' named entity 'standard curve' named entity 'adsorption' named entity 'RNA' named entity 'BSA' named entity 'RNA extraction' named entity 'sputum' named entity 'SARS-CoV-2' named entity 'RT-qPCR' named entity 'virus' named entity 'nCoV' named entity 'nCoV' named entity 'SARS-CoV-2' named entity 'cruise ship' named entity 'Australia' named entity 'severe pneumonia' named entity 'SARS-CoV-2' named entity 'SARS-CoV-2' named entity 'Integrated DNA Technologies' named entity 'commercial aircraft' named entity 'cruise ship' named entity 'air travel' named entity 'RT-qPCR' named entity 'CDC' named entity 'CDC' named entity 'Australia' named entity 'reverse transcriptase' named entity 'PCR' named entity 'nasopharyngeal' named entity 'quantification cycle' named entity 'RNA' named entity 'disinfectants' named entity 'informs'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software