About: Silencing of SARS-CoV spike gene by small interfering RNA in HEK 293T cells

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About: Silencing of SARS-CoV spike gene by small interfering RNA in HEK 293T cells Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Silencing of SARS-CoV spike gene by small interfering RNA in HEK 293T cells
Creator	Zhao, Ping Luan, Jie Qi, Zhong-Tian Zhao, Lan-Juan Zhang, Xiao-Lian Cao, Ming-Mei Qin, Zhao-Ling Yu, Jian-Guo
Source	Elsevier; Medline; PMC
abstract	Abstract Two candidate small interfering RNAs (siRNAs) corresponding to severe acute respiratory syndrome-associated coronavirus (SARS-CoV) spike gene were designed and in vitro transcribed to explore the possibility of silencing SARS-CoV S gene. The plasmid pEGFP-optS, which contains the codon-optimized SARS-CoV S gene and expresses spike-EGFP fusion protein (S-EGFP) as silencing target and expressing reporter, was transfected with siRNAs into HEK 293T cells. At various time points of posttransfection, the levels of S-EGFP expression and amounts of spike mRNA transcript were detected by fluorescence microscopy, flow cytometry, Western blot, and real-time quantitative PCR, respectively. The results showed that the cells transfected with pEGFP-optS expressed S-EGFP fusion protein at a higher level compared with those transfected with pEGFP-S, which contains wildtype SARS-CoV spike gene sequence. The green fluorescence, mean fluorescence intensity, and SARS-CoV S RNA transcripts were found significantly reduced, and the expression of SARS-CoV S glycoprotein was strongly inhibited in those cells co-transfected with either EGFP- or S-specific siRNAs. Our findings demonstrated that the S-specific siRNAs used in this study were able to specifically and effectively inhibit SARS-CoV S glycoprotein expression in cultured cells through blocking the accumulation of S mRNA, which may provide an approach for studies on the functions of SARS-CoV S gene and development of novel prophylactic or therapeutic agents for SARS-CoV.
has issue date	2004-11-26(xsd:dateTime)
bibo:doi	10.1016/j.bbrc.2004.09.180
bibo:pmid	15504339
has license	els-covid
sha1sum (hex)	a01a6cdf83b4f97c924da009bc034e8d01ad6f10
schema:url	https://doi.org/10.1016/j.bbrc.2004.09.180
resource representing a document's title	Silencing of SARS-CoV spike gene by small interfering RNA in HEK 293T cells
has PubMed Central identifier	PMC7092946
has PubMed identifier	15504339
schema:publication	Biochemical and Biophysical Research Communications
resource representing a document's body	covid:a01a6cdf83b4f97c924da009bc034e8d01ad6f10#body_text
is schema:about of	named entity 'SARS-CoV' named entity 'SARS-CoV' named entity 'SARS-CoV' named entity 'wildtype' named entity 'RNA' named entity 'expresses' named entity 'gene' named entity 'SARS-COV' named entity 'HEK' named entity 'INHIBITED' named entity 'THERAPEUTIC AGENTS' named entity 'SPIKE' named entity 'HIGHER' named entity 'GREEN ' named entity 'TRANSCRIBED' named entity 'LEVEL' named entity 'TIME' named entity 'HEK' named entity 'REAL-TIME QUANTITATIVE PCR' named entity 'USED' named entity 'CELLS' named entity 'SARS-COV' named entity 'THE GREEN' named entity '293T CELLS' named entity 'SMALL INTERFERING RNA' named entity 'EGFP' named entity 'STUDY' named entity 'CONTAINS' named entity 'EXPRESSED' named entity 'DETECTED' named entity 'CANDIDATE' named entity 'SILENCING' named entity 'GENE' named entity 'LEVELS' named entity 'TRANSCRIPT' named entity 'WESTERN BLOT' named entity 'FLUORESCENCE' named entity 'MEAN FLUORESCENCE INTENSITY' named entity 'GENE' named entity 'SPIKE' named entity 'CULTURED CELLS' named entity 'POSSIBILITY OF' named entity 'IN VITRO' named entity 'ASSOCIATED' named entity 'CODON' named entity 'FOUND' named entity '28S' named entity 'OUR' named entity '293T CELLS' named entity 'FUNCTIONS' named entity 'FLOW CYTOMETRY' named entity 'BLOCKING' named entity 'STUDIES' named entity 'GLYCOPROTEIN' named entity 'REDUCED' named entity 'APPROACH' named entity 'PROVIDE' named entity 'TARGET' named entity 'RESULTS' named entity 'FLUORESCENCE MICROSCOPY' named entity 'SIRNAS' named entity 'EITHER' named entity 'ACCUMULATION' named entity 'REPORTER' named entity 'SMALL INTERFERING RNAS' named entity 'SEVERE ACUTE RESPIRATORY SYNDROME' named entity 'NOVEL' named entity 'DEMONSTRATED' named entity 'PROTEIN ' named entity 'SILENCING' named entity 'EXPRESSION' named entity 'COMPARED' named entity 'FINDINGS' named entity 'FUSION PROTEIN'

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