About: Characterizing the rapid spread of porcine epidemic diarrhea virus (PEDV) through an animal food manufacturing facility

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About: Characterizing the rapid spread of porcine epidemic diarrhea virus (PEDV) through an animal food manufacturing facility Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Characterizing the rapid spread of porcine epidemic diarrhea virus (PEDV) through an animal food manufacturing facility
Creator	Chen, Qi Gauger, Phillip Zhang, Jianqiang Main, Rodger Bai, Jianfa Poulsen, Elizabeth Ramirez, Alejandro Cochrane, Roger Jones, Cassandra Derscheid, Rachel Magstadt, Drew Huss, Anne Schumacher, Loni Dritz, Steve Stark, Charles Woodworth, Jason
Source	Medline; PMC
abstract	New regulatory and consumer demands highlight the importance of animal feed as a part of our national food safety system. Porcine epidemic diarrhea virus (PEDV) is the first viral pathogen confirmed to be widely transmissible in animal food. Because the potential for viral contamination in animal food is not well characterized, the objectives of this study were to 1) observe the magnitude of virus contamination in an animal food manufacturing facility, and 2) investigate a proposed method, feed sequencing, to decrease virus decontamination on animal food-contact surfaces. A U.S. virulent PEDV isolate was used to inoculate 50 kg swine feed, which was mixed, conveyed, and discharged into bags using pilot-scale feed manufacturing equipment. Surfaces were swabbed and analyzed for the presence of PEDV RNA by quantitative real-time polymerase chain reaction (qPCR). Environmental swabs indicated complete contamination of animal food-contact surfaces (0/40 vs. 48/48, positive baseline samples/total baseline samples, positive subsequent samples/total subsequent samples, respectively; P < 0.05) and near complete contamination of non-animal food-contact surfaces (0/24 vs. 16/18, positive baseline samples/total baseline samples, positive subsequent samples/total subsequent samples, respectively; P < 0.05). Flushing animal food-contact surfaces with low-risk feed is commonly used to reduce cross-contamination in animal feed manufacturing. Thus, four subsequent 50 kg batches of virus-free swine feed were manufactured using the same system to test its impact on decontaminating animal food-contact surfaces. Even after 4 subsequent sequences, animal food-contact surfaces retained viral RNA (28/33 positive samples/total samples), with conveying system being more contaminated than the mixer. A bioassay to test infectivity of dust from animal food-contact surfaces failed to produce infectivity. This study demonstrates the potential widespread viral contamination of surfaces in an animal food manufacturing facility and the difficulty of removing contamination using conventional feed sequencing, which underscores the importance for preventing viruses from entering and contaminating such facilities.
has issue date	2017-11-02(xsd:dateTime)
bibo:doi	10.1371/journal.pone.0187309
bibo:pmid	29095859
has license	cc-by
sha1sum (hex)	a0acb3cda6288d12ce50136a88c013a3098ee3fd
schema:url	https://doi.org/10.1371/journal.pone.0187309
resource representing a document's title	Characterizing the rapid spread of porcine epidemic diarrhea virus (PEDV) through an animal food manufacturing facility
has PubMed Central identifier	PMC5667810
has PubMed identifier	29095859
schema:publication	PLoS One
resource representing a document's body	covid:a0acb3cda6288d12ce50136a88c013a3098ee3fd#body_text
is schema:about of	named entity 'food' named entity 'contamination' named entity 'chain reaction' named entity 'total' named entity 'national' named entity 'study' named entity 'Flushing' named entity 'surfaces' covid:arg/a0acb3cda6288d12ce50136a88c013a3098ee3fd named entity 'SWINE FEED' named entity 'RETAINED' named entity 'FLUSHING' named entity 'OBSERVE' named entity 'NEW' named entity 'HTTPS' named entity 'CONSUMER' named entity 'PART OF' named entity 'FEED' named entity 'VIRAL PATHOGEN' named entity 'INFECTIVITY' named entity 'PORCINE EPIDEMIC DIARRHEA VIRUS' named entity 'VIRUS CONTAMINATION' named entity 'NOT WELL' named entity 'CONVEYING' named entity 'SUBSEQUENT' named entity 'USED' named entity 'NON-' named entity 'EQUIPMENT' named entity 'VIRAL RNA' named entity 'REGULATORY' named entity '0.05' named entity 'NATIONAL' named entity 'U.S.' named entity 'ITS' named entity 'MAGNITUDE' named entity 'SCALE' named entity 'IMPACT' named entity 'TO PRODUCE' named entity 'POTENTIAL' named entity 'BASELINE' named entity 'CONTAMINATED' named entity 'CONTAMINATION' named entity 'PRESENCE OF' named entity 'FAILED' named entity 'WIDESPREAD' named entity 'QUANTITATIVE REAL-TIME POLYMERASE CHAIN REACTION' named entity 'SWABS' named entity 'BIOASSAY' named entity 'OBJECTIVES' named entity 'TO DECREASE' named entity 'SWABBED' named entity 'RISK' named entity 'CROSS' named entity 'BEING' named entity 'SPREAD' named entity 'RAPID' named entity 'MANUFACTURING FACILITY' named entity 'ANIMAL FOOD' named entity 'PORCINE EPIDEMIC DIARRHEA VIRUS' named entity 'INVESTIGATE' named entity 'FREE' named entity 'NEAR COMPLETE' named entity 'SEQUENCES' named entity 'MIXER' named entity 'ANIMAL' named entity 'FOOD SAFETY SYSTEM'

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