About: Phage M13KO7 detection with biosensor based on imaging ellipsometry and AFM microscopic confirmation

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About: Phage M13KO7 detection with biosensor based on imaging ellipsometry and AFM microscopic confirmation Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Phage M13KO7 detection with biosensor based on imaging ellipsometry and AFM microscopic confirmation
Creator	Feng, Jing Jin, Gang Qi, Cai Lin, Yi Meng, Yong-Hong Wan, Li-Jun Wang, Zhan-Hui Yan, Xi-Yun Zhu, Chuan-Feng
Source	Elsevier; Medline; PMC
abstract	Abstract A rapid detection and identification of pathogens is important for minimizing transfer and spread of disease. A label-free and multiplex biosensor based on imaging ellipsometry (BIE) had been developed for the detection of phage M13KO7. The surface of silicon wafer is modified with aldehyde, and proteins can be patterned homogeneously and simultaneously on the surface of silicon wafer in an array format by a microfluidic system. Avidin is immobilized on the surface for biotin-anti-M13 immobilization by means of interaction between avidin and biotin, which will serve as ligand against phage M13KO7. Phages M13KO7 are specifically captured by the ligand when phage M13KO7 solution passes over the surface, resulting in a significant increase of mass surface concentration of the anti-M13 binding phage M13KO7 layer, which could be detected by imaging ellipsometry with a sensitivity of 109 pfu/ml. Moreover, atomic force microscopy is also used to confirm the fact that phage M13KO7 has been directly captured by ligands on the surface. It indicates that BIE is competent for direct detection of phage M13KO7 and has potential in the field of virus detection.
has issue date	2009-03-31(xsd:dateTime)
bibo:doi	10.1016/j.virusres.2008.11.010
bibo:pmid	19071167
has license	els-covid
sha1sum (hex)	a0b143f07ee07b0ce90b4b3c74a4f123b9322160
schema:url	https://doi.org/10.1016/j.virusres.2008.11.010
resource representing a document's title	Phage M13KO7 detection with biosensor based on imaging ellipsometry and AFM microscopic confirmation
has PubMed Central identifier	PMC7114425
has PubMed identifier	19071167
schema:publication	Virus Research
resource representing a document's body	covid:a0b143f07ee07b0ce90b4b3c74a4f123b9322160#body_text
is schema:about of	named entity 'immobilized' named entity 'imaging' named entity 'detection' named entity 'surface' named entity 'microscopic' covid:arg/a0b143f07ee07b0ce90b4b3c74a4f123b9322160 named entity 'silicon wafer' named entity 'binding' named entity 'surface' named entity 'disease' named entity 'surface' named entity 'phage' named entity 'atomic force microscopy' named entity 'biotin' named entity 'ellipsometry' named entity 'ligands' named entity 'phage' named entity 'ligand' named entity 'biosensor' named entity 'ultrasonic' named entity 'quartz crystal microbalance' named entity 'PBS' named entity 'silicon wafer' named entity 'antibody' named entity 'filamentous phage' named entity 'AFM' named entity 'viruses' named entity 'AFM' named entity 'silicon' named entity 'phage' named entity 'antibody' named entity 'deionized water' named entity 'microscope' named entity 'molecule' named entity 'virus' named entity 'avidin' named entity 'phage' named entity 'antibody' named entity 'atomic force microscope' named entity 'phage' named entity 'phage' named entity 'QCM' named entity 'viruses' named entity 'protein' named entity 'avidin' named entity 'antibody' named entity 'phage' named entity 'Silicon wafers' named entity 'NH 2' named entity 'chemical groups' named entity 'high molecular weight' named entity 'ligand' named entity 'AFM' named entity 'QCM' named entity 'APTES' named entity 'ellipsometry' named entity 'biotin' named entity 'silicon wafer' named entity 'Chinese Academy of Sciences' named entity 'PCR' named entity 'phage' named entity 'filamentous phage' named entity 'molecule' named entity 'wafer' named entity 'absolute ethanol' named entity 'absolute ethanol' named entity 'aqueous solution' named entity 'substrate' named entity 'ultra-thin' named entity 'viruses' named entity 'covalently' named entity 'Institute of Chemistry' named entity 'Clinical diagnosis'

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