Facets (new session)
Description
Metadata
Settings
owl:sameAs
Inference Rule:
b3s
b3sifp
dbprdf-label
facets
http://dbpedia.org/resource/inference/rules/dbpedia#
http://dbpedia.org/resource/inference/rules/opencyc#
http://dbpedia.org/resource/inference/rules/umbel#
http://dbpedia.org/resource/inference/rules/yago#
http://dbpedia.org/schema/property_rules#
http://www.ontologyportal.org/inference/rules/SUMO#
http://www.ontologyportal.org/inference/rules/WordNet#
http://www.w3.org/2002/07/owl#
ldp
oplweb
skos-trans
virtrdf-label
None
About:
T. cruzi OligoC-TesT: A Simplified and Standardized Polymerase Chain Reaction Format for Diagnosis of Chagas Disease
Goto
Sponge
NotDistinct
Permalink
An Entity of Type :
schema:ScholarlyArticle
, within Data Space :
wasabi.inria.fr
associated with source
document(s)
Type:
Academic Article
research paper
schema:ScholarlyArticle
New Facet based on Instances of this Class
Attributes
Values
type
Academic Article
research paper
schema:ScholarlyArticle
isDefinedBy
Covid-on-the-Web dataset
has title
T. cruzi OligoC-TesT: A Simplified and Standardized Polymerase Chain Reaction Format for Diagnosis of Chagas Disease
Creator
Apt, Werner
Coronado, Ximena
Deborggraeve, Stijn
Dujardin, Jean-Claude
Herdewijn, Piet
Laurent, Thierry
Leclipteux, Thierry
Mertens, Pascal
Solari, Aldo
Stessens, Tim
Zulantay, Ines
Bü Scher, Philippe
Source
Medline; PMC
abstract
BACKGROUND: PCR has evolved into one of the most promising tools for T. cruzi detection in the diagnosis and control of Chagas disease. However, general use of the technique is hampered by its complexity and the lack of standardization. METHODOLOGY: We here present the development and phase I evaluation of the T. cruzi OligoC-TesT, a simple and standardized dipstick format for detection of PCR amplified T. cruzi DNA. The specificity and sensitivity of the assay were evaluated on blood samples from 60 Chagas non-endemic and 48 endemic control persons and on biological samples from 33 patients, 7 reservoir animals, and 14 vectors collected in Chile. PRINCIPAL FINDINGS: The lower detection limits of the T. cruzi OligoC-TesT were 1 pg and 1 to 10 fg of DNA from T. cruzi lineage I and II, respectively. The test showed a specificity of 100% (95% confidence interval [CI]: 96.6%–100%) on the control samples and a sensitivity of 93.9% (95% CI: 80.4%–98.3%), 100% (95% CI: 64.6%–100%), and 100% (95% CI: 78.5%–100%) on the human, rodent, and vector samples, respectively. CONCLUSIONS: The T. cruzi OligoC-TesT showed high sensitivity and specificity on a diverse panel of biological samples. The new tool is an important step towards simplified and standardized molecular diagnosis of Chagas disease.
has issue date
2009-06-02
(
xsd:dateTime
)
bibo:doi
10.1371/journal.pntd.0000450
bibo:pmid
19503815
has license
cc-by
sha1sum (hex)
a0c2551d031c6dd3f59392c3f75dffc7b694a186
schema:url
https://doi.org/10.1371/journal.pntd.0000450
resource representing a document's title
T. cruzi OligoC-TesT: A Simplified and Standardized Polymerase Chain Reaction Format for Diagnosis of Chagas Disease
has PubMed Central identifier
PMC2685481
has PubMed identifier
19503815
schema:publication
PLoS Negl Trop Dis
resource representing a document's body
covid:a0c2551d031c6dd3f59392c3f75dffc7b694a186#body_text
is
schema:about
of
named entity 'CHAGAS DISEASE'
named entity 'PCR'
named entity 'POLYMERASE CHAIN REACTION'
named entity 'FORMAT'
named entity 'ONE OF'
named entity 'STANDARDIZATION'
named entity 'BACKGROUND'
named entity 'DETECTION'
named entity 'EVOLVED'
named entity 'TECHNIQUE'
named entity 'TOOLS'
named entity 'COMPLEXITY'
named entity 'FOR DIAGNOSIS'
named entity 'TEST'
named entity 'USE OF'
named entity 'LACK'
named entity 'PCR'
named entity 'ITS'
named entity 'CHAGAS DISEASE'
named entity 'CONTROL'
named entity 'GENERAL'
named entity 'DIAGNOSIS'
covid:arg/a0c2551d031c6dd3f59392c3f75dffc7b694a186
named entity 'Simplified'
named entity 'T. cruzi'
named entity 'Chagas Disease'
named entity 'Chagas'
named entity 'PCR'
named entity 'Polymerase Chain Reaction'
named entity 'T. cruzi'
named entity 'Chagas disease'
named entity 'evolved'
named entity 'standardization'
named entity 'T. cruzi'
named entity 'promising'
named entity 'T. cruzi'
named entity 'Standardized'
named entity 'diagnosis'
named entity 'general'
named entity 'Background'
named entity 'However'
named entity 'complexity'
named entity 'Polymerase Chain Reaction'
named entity 'Diagnosis'
named entity 'Chagas Disease'
nodeID://b56588810
nodeID://b57242222
nodeID://b57279514
nodeID://b57303638
nodeID://b57347386
nodeID://b57624766
nodeID://b58055942
nodeID://b58056366
nodeID://b58069722
nodeID://b58139246
is
wasQuotedFrom
of
covid:arg/a0c2551d031c6dd3f59392c3f75dffc7b694a186/0
is
part of
of
covid:a0c2551d031c6dd3f59392c3f75dffc7b694a186#body_text
BACKGROUND: PCR has evolved into one of the most promising tools for T. cruzi detection in the diagnosis and control of Chagas disease. However, general use of the technique is hampered by its complexity and the lack of standardization. METHODOLOGY: We here present the development and phase I evaluation of the T. cruzi OligoC-TesT, a simple and standardized dipstick format for detection of PCR amplified T. cruzi DNA. The specificity and sensitivity of the assay were evaluated on blood samples from 60 Chagas non-endemic and 48 endemic control persons and on biological samples from 33 patients, 7 reservoir animals, and 14 vectors collected in Chile. PRINCIPAL FINDINGS: The lower detection limits of the T. cruzi OligoC-TesT were 1 pg and 1 to 10 fg of DNA from T. cruzi lineage I and II, respectively. The test showed a specificity of 100% (95% confidence interval [CI]: 96.6%–100%) on the control samples and a sensitivity of 93.9% (95% CI: 80.4%–98.3%), 100% (95% CI: 64.6%–100%), and 100% (95% CI: 78.5%–100%) on the human, rodent, and vector samples, respectively. CONCLUSIONS: The T. cruzi OligoC-TesT showed high sensitivity and specificity on a diverse panel of biological samples. The new tool is an important step towards simplified and standardized molecular diagnosis of Chagas disease.
T. cruzi OligoC-TesT: A Simplified and Standardized Polymerase Chain Reaction Format for Diagnosis of Chagas Disease
Faceted Search & Find service v1.13.91 as of Mar 24 2020
Alternative Linked Data Documents:
Sponger
|
ODE
Content Formats:
RDF
ODATA
Microdata
About
OpenLink Virtuoso
version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software