About: Recognition of Lys48-linked di-ubiquitin and deubiquitinating activities of the SARS coronavirus papain-like protease

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About: Recognition of Lys48-linked di-ubiquitin and deubiquitinating activities of the SARS coronavirus papain-like protease Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Recognition of Lys48-linked di-ubiquitin and deubiquitinating activities of the SARS coronavirus papain-like protease
Creator	Lima, Ekkebus, Huib Huang, Christopher Mikló S Bé Ké S, Gerbrand Ovaa, Tony Van Der Heden Van Noort, Reggy
source	Elsevier; Medline; PMC
abstract	Deubiquitinating enzymes (DUBs) recognize and cleave linkage-specific poly-ubiquitin (polyUb) chains, but mechanisms underlying specificity remain elusive in many cases. The Severe Acute Respiratory Syndrome (SARS) coronavirus papain-like protease (PLpro) is a DUB that cleaves ISG15, a two domain Ub-like protein, and Lys48-linked polyUb chains, releasing diUb(Lys48) products. To elucidate this specificity, we report the 2.85 Å crystal structure of SARS PLpro bound to a diUb(Lys48) activity-based probe. SARS PLpro binds diUb(Lys48) in an extended conformation via two contact sites, S1 and S2, which are proximal and distal to the active site, respectively. We show that specificity for polyUb(Lys48) chains is predicated on contacts in the S2 site and enhanced by an S1–S1′ preference for a Lys48 linkage across the active site. In contrast, ISG15 specificity is dominated by contacts in the S1 site. Determinants revealed for polyUb(Lys48) specificity should prove useful in understanding PLpro deubiquitinating activities in coronavirus infections.
has issue date	2016-05-01(xsd:dateTime)
bibo:doi	10.1016/j.molcel.2016.04.016
bibo:pmid	27203180
has license	hybrid-oa
sha1sum (hex)	a1806c0fe27f146c54fd656e480a882a3e561fa2
schema:url	https://doi.org/10.1016/j.molcel.2016.04.016
resource representing a document's title	Recognition of Lys48-linked di-ubiquitin and deubiquitinating activities of the SARS coronavirus papain-like protease
has PubMed Central identifier	PMC4875570
has PubMed identifier	27203180
schema:publication	Molecular Cell
resource representing a document's body	covid:a1806c0fe27f146c54fd656e480a882a3e561fa2#body_text
is schema:about of	named entity 'labels' named entity 'manners' named entity 'DEUBIQUITINATING' named entity 'RECOGNITION' named entity 'D A' named entity 'CHAINS' named entity 'ACTIVITY' named entity 'STRUCTURE' named entity 'REVEALS' named entity 'SARS CORONAVIRUS' named entity 'PROBE' named entity 'UBIQUITIN' named entity 'LIKE' named entity 'ACTIVITIES' named entity 'CONFORMATION' named entity 'INTERACTIONS' named entity 'PROTEASE' named entity 'PAPAIN' named entity 'DIUBIQUITIN' named entity 'LINKAGE' named entity 'POLYUBIQUITIN' named entity 'SARS' named entity 'BASED' named entity 'SPECIFIC' named entity 'COMPLEX' named entity 'MAKE' named entity 'DISTINCT' named entity 'ISG15' named entity 'LABELS' named entity 'EXTENDED' named entity 'SARS' named entity 'PLpro' named entity 'S3G' named entity 'conformation' named entity 'ISG15' named entity 'PLpro' named entity 'virus' named entity 'polyubiquitin' named entity 'binding motif' named entity 'PLpro' named entity 'kinetics' named entity 'SARS' named entity 'PLpro' named entity 'triazole' named entity 'SARS' named entity 'PLpro' named entity 'SARS' named entity 'PLpro' named entity 'hydrolysis' named entity 'PLpro' named entity 'molecular basis' named entity 'SARS' named entity 'cell culture' named entity 'Met1' named entity 'PLpro' named entity 'loss-of-function' named entity 'PLpro' named entity 'catalytic core' named entity 'IkBa' named entity 'PLpro' named entity 'recombinant' named entity 'SARS' named entity 'ISG15' named entity 'SARS' named entity 'SARS' named entity 'substrates' named entity 'asymmetric unit' named entity 'NaCl' named entity 'ISG15' named entity 'PLpro' named entity 'papain' named entity 'PLpro' named entity 'Bhoj' named entity 'SARS' named entity 'SARS' named entity 'isopeptide'

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