About: Structures of human antibodies bound to SARS-CoV-2 spike reveal common epitopes and recurrent features of antibodies

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About: Structures of human antibodies bound to SARS-CoV-2 spike reveal common epitopes and recurrent features of antibodies Goto Sponge NotDistinct Permalink

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Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Structures of human antibodies bound to SARS-CoV-2 spike reveal common epitopes and recurrent features of antibodies
Creator	Bjorkman, Pamela Nussenzweig, Michel West, Anthony Bieniasz, Paul Barnes, Christopher Barnes, C Caskey, Marina Finkin, Shlomo Gaebler, Christian Gristick, Harry Hatziioannou, Theodora Hoffman, Pauline Hoffmann, Magnus Huey-Tubman, Kathryn Hurley, Arlene Koranda, Nicholas Lorenzi, Cetrulo Lorenzi, Julio Millard, Katrina Muecksch, Frauke Robbiani, Davide Schmidt, Fabian Sharaf, Naima Weisblum, Yiska Bieniasz, T Bjorkman, M Finkin, J Gaebler, H Gristick, N Hoffman, N Hoffmann, K Hurley, T Hägglöf, S Hägglöf, Thomas Koranda, P Millard, A Nussenzweig, D Robbiani, M Schmidt, Y Sharaf, M Weisblum, K
Source	Elsevier; Medline; PMC
abstract	Summary Neutralizing antibody responses to coronaviruses mainly target the receptor-binding domain (RBD) of the trimeric spike. Here, we characterized polyclonal IgGs and Fabs from COVID-19 convalescent individuals for recognition of coronavirus spikes. Plasma IgGs differed in their focus on RBD epitopes, recognition of alpha- and beta-coronaviruses, and contributions of avidity to increased binding/neutralization of IgGs over Fabs. Using electron microscopy, we examined specificities of polyclonal plasma Fabs, revealing recognition of both S1A and RBD epitopes on SARS-CoV-2 spike. Moreover, a 3.4Å cryo-EM structure of a neutralizing monoclonal Fab-spike complex revealed an epitope that blocks ACE2 receptor binding. Modeling based on these structures suggested different potentials for inter-spike crosslinking by IgGs on viruses and that characterized IgGs would not be affected by identified SARS-CoV-2 spike mutations. Overall, our studies structurally define a recurrent anti-SARS-CoV-2 antibody class derived from VH3-53/VH3-66 and similarity to a SARS-CoV VH3-30 antibody, providing criteria for evaluating vaccine-elicited antibodies.
has issue date	2020-06-24(xsd:dateTime)
bibo:doi	10.1016/j.cell.2020.06.025
bibo:pmid	32645326
has license	els-covid
sha1sum (hex)	a23dd75b617ba129523932a693f6616cf27885ba
schema:url	https://doi.org/10.1016/j.cell.2020.06.025
resource representing a document's title	Structures of human antibodies bound to SARS-CoV-2 spike reveal common epitopes and recurrent features of antibodies
has PubMed Central identifier	PMC7311918
has PubMed identifier	32645326
schema:publication	Cell
resource representing a document's body	covid:a23dd75b617ba129523932a693f6616cf27885ba#body_text
is schema:about of	named entity 'CRITERIA' named entity 'REVEAL' named entity 'Pamela J. Bjorkman' named entity 'antibodies' named entity 'caltech' named entity 'SARS-CoV-2' named entity 'antibody' named entity 'SARS-CoV-2' named entity 'antibodies' named entity 'amino acid' named entity 'atom' named entity 'B38' named entity 'PDB' named entity 'SARS-CoV-2' named entity 'RBD' named entity 'subcloned' named entity 'Fab' named entity 'neutralizing antibodies' named entity 'RBD' named entity 'VH1' named entity 'CSIB' named entity 'SARS-CoV-2' named entity 'AviTag' named entity 'antibody' named entity 'integral membrane protein' named entity 'cell lines' named entity 'Superose' named entity 'HEK293T' named entity 'Fab' named entity 'epitope' named entity 'BetaGlobin' named entity 'ACE2 receptor' named entity 'COVID-19' named entity 'cell membrane' named entity 'HCoV-NL63' named entity 'SARS-CoV' named entity 'Fab' named entity 'Sigma-Aldrich' named entity 'multimeric' named entity 'plasma' named entity 'COVID-19'

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