About: Development of a sensitive monoclonal antibody-based sandwich ELISA to detect Vip3Aa in genetically modified crops

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About: Development of a sensitive monoclonal antibody-based sandwich ELISA to detect Vip3Aa in genetically modified crops Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Development of a sensitive monoclonal antibody-based sandwich ELISA to detect Vip3Aa in genetically modified crops
Creator	Zhe, Zhang Liu, Weixiao Chao, Liu Wujun, Jin Xuri, Liu
Source	PMC
abstract	OBJECTIVES: To develop a sensitive monoclonal antibody-based sandwich enzyme-linked immunosorbent assay (ELISA) to detect Vip3Aa in genetically modified (GM) crops and their products. RESULTS: Vegetative insecticidal proteins (Vips) are secreted by Bacillus thuringiensis (Bt) and are known to be toxic to Lepidoptera species. Vip3Aa family proteins, Vip3Aa19 and Vip3Aa20, were successfully applied in GM crops to confer an effective and persistent insecticidal resistance. A sensitive monoclonal antibody-based sandwich ELISA was developed to detect Vip3Aa in GM crops and their products. Two monoclonal antibodies were raised against the overexpressed and purified His-Vip3Aa20, were purified from mouse ascites and characterized. A sandwich ELISA method was developed using the 2G3-1D7 monoclonal antibody for capture and the biotin-labeled 1F9-1F5 monoclonal antibody for detection of Vip3Aa20. The linear detection range of the method was found to be approximately 31.25–500 pg/ml, with a sensitivity of 10.24 pg/ml. CONCLUSIONS: The established ELISA was effective for detecting Vip3Aa family proteins other than Vip3Aa8, and was successfully applied in the detection of Vip3Aa20 and Vip3Aa19 expressed in transgenic maize and cotton.
has issue date	2020-03-05(xsd:dateTime)
bibo:doi	10.1007/s10529-020-02854-9
bibo:pmid	32140882
has license	cc-by
sha1sum (hex)	a3db8a4eb4bc4757a1e3465d08de0ce36de3cb18
schema:url	https://doi.org/10.1007/s10529-020-02854-9
resource representing a document's title	Development of a sensitive monoclonal antibody-based sandwich ELISA to detect Vip3Aa in genetically modified crops
has PubMed Central identifier	PMC7354279
has PubMed identifier	32140882
schema:publication	Biotechnol Lett
resource representing a document's body	covid:a3db8a4eb4bc4757a1e3465d08de0ce36de3cb18#body_text
is schema:about of	named entity 'GM crops' named entity 'transgenic maize' named entity 'SANDWICH ELISA' named entity 'MAIZE' named entity 'ESTABLISHED' named entity 'ASCITES' named entity 'TO DETECT' named entity 'COTTON' named entity 'RESULTS' named entity 'PRODUCTS' named entity 'OTHER THAN' named entity 'SENSITIVE' named entity 'monoclonal antibody' named entity 'develop' named entity 'proteins' named entity 'enzyme-linked immunosorbent assay' named entity 'Results' named entity 'confer' named entity 'Two' named entity 'ascites' named entity 'monoclonal' named entity 'transgenic maize' named entity 'GM crops' named entity 'Lepidoptera' named entity 'monoclonal antibody' named entity 'insecticidal' named entity 'insecticidal' named entity '2.1' named entity 'Protein' named entity 'mAb' named entity 'molecular weights' named entity 'transgenic crops' named entity 'protein' named entity 'protein' named entity 'inclusion bodies' named entity 'ascitic fluid' named entity 'sandwich ELISA' named entity 'antibody' named entity 'DMEM' named entity 'spleen' named entity 'supernatant' named entity 'Agrotis ipsilon' named entity 'RPMI' named entity 'biotin' named entity 'skim milk' named entity 'Hybridomas' named entity 'insecticidal' named entity 'denature' named entity 'hybridoma' named entity 'clones' named entity 'E. coli' named entity 'hybridoma' named entity 'horseradish peroxidase' named entity 'maize' named entity 'expression vector' named entity 'Bacillus thuringiensis' named entity 'Lepidoptera' named entity 'aminopterin' named entity 'antibody' named entity 'IgG' named entity 'Antibody' named entity 'standard curve' named entity 'protein' named entity '450 nm' named entity 'protein' named entity 'environmental safety' named entity 'absorbance' named entity 'cross-reactivity' named entity 'protein' named entity 'ascitic fluid' named entity '450 nm' named entity 'sandwich ELISA' named entity 'immunogen' named entity 'FCS' named entity 'subcloned' named entity 'monoclonal antibodies' named entity 'polyethylene glycol'

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