About: The potential of the human immune system to develop broadly neutralizing HIV-1 antibodies: implications for vaccine development

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About: The potential of the human immune system to develop broadly neutralizing HIV-1 antibodies: implications for vaccine development Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	The potential of the human immune system to develop broadly neutralizing HIV-1 antibodies: implications for vaccine development
Creator	Chen, Zhiwei Xu, Jianqing Zhang, Yu Shao, Yiming Li, Jingjing Zhang, Mei-Yun Yuan, Tingting Zhang, Yanyu
topic	covid:a41e8928ee5cb212550be0620946d6e62c34b001#this
Source	Medline; PMC
abstract	OBJECTIVES AND DESIGN: Developing an effective HIV-1 vaccine that elicits broadly neutralizing HIV-1 human antibodies (bnAbs) remains a challenging goal. Extensive studies on HIV-1 have revealed various strategies employed by the virus to escape host immune surveillance. Here, we investigated the human antibody gene repertoires of uninfected and HIV-1-infected individuals at genomic DNA (gDNA) and cDNA levels by deep sequencing followed by high-throughput sequence analysis to determine the frequencies of putative germline antibody genes of known HIV-1 monoclonal bnAbs (bnmAbs). METHODS: Combinatorial gDNA and cDNA antibody libraries were constructed using the gDNAs and mRNAs isolated from uninfected and HIV-1-infected human peripheral blood mononuclear cells (PBMCs). All libraries were deep sequenced and sequences analysed using IMGT/HighV-QUEST software (http://imgt.org/HighV-QUEST/index.action). The frequencies of putative germline antibodies of known bnmAbs in the gDNA and cDNA libraries were determined. RESULTS AND CONCLUSION: The human gDNA antibody libraries were more diverse in heavy and light chain V-gene lineage usage than the cDNA libraries, indicating that the human gDNA antibody gene repertoires may have more potential than the cDNA repertoires to develop HIV-1 bnAbs. The frequencies of the heavy and kappa and lambda light chain variable regions with identical V(D)J recombinations to known HIV-1 bnmAbs were extremely low in human antibody gene repertoires. However, we found relatively high frequencies of the heavy and kappa and lambda light chain variable regions that used the same V-genes and had the same CDR3 lengths as known HIV-1 bnmAbs regardless of (D)J-gene usage. B-cells bearing B-cell receptors of such heavy and kappa and lambda light chain variable regions may be stimulated to induce HIV-1 bnAbs.
has issue date	2013-10-23(xsd:dateTime)
bibo:doi	10.1097/qad.0000000000000015
bibo:pmid	24100711
has license	cc-by-nc-nd
sha1sum (hex)	a41e8928ee5cb212550be0620946d6e62c34b001
schema:url	https://doi.org/10.1097/qad.0000000000000015
resource representing a document's title	The potential of the human immune system to develop broadly neutralizing HIV-1 antibodies: implications for vaccine development
has PubMed Central identifier	PMC3815086
has PubMed identifier	24100711
schema:publication	AIDS
resource representing a document's body	covid:a41e8928ee5cb212550be0620946d6e62c34b001#body_text
is http://vocab.deri.ie/void#inDataset of	https://covidontheweb.inria.fr:4443/about/id/http/ns.inria.fr/covid19/a41e8928ee5cb212550be0620946d6e62c34b001
is schema:about of	named entity 'host' named entity 'sequence analysis' named entity 'HIV-1' named entity 'HIV-1' named entity 'human' covid:arg/a41e8928ee5cb212550be0620946d6e62c34b001 named entity 'human' named entity 'antibody' named entity 'deep sequencing' named entity 'HIV-1' named entity 'antibody' named entity 'HIV-1' named entity 'HIV-1' named entity 'antibody' named entity 'HIV-1' named entity 'cDNA' named entity 'HIV-1' named entity 'sequence analysis' named entity 'light chain' named entity 'gDNA' named entity 'A401' named entity 'cDNA libraries' named entity 'gDNA' named entity 'SOE' named entity 'deep sequencing' named entity 'constant regions' named entity 'binding activity' named entity 'Deep sequencing' named entity 'variable regions' named entity 'VDJ recombination' named entity 'gene family' named entity 'covalently linked' named entity 'gel extraction' named entity 'light chains' named entity 'HIV-1' named entity 'antibodies' named entity 'germline' named entity 'reverse transcribe' named entity 'heavy chain' named entity 'V(D)J' named entity 'infection' named entity 'IGHD' named entity 'cDNA libraries' named entity 'cDNA' named entity 'variable regions' named entity 'heavy chain' named entity 'gDNA' named entity 'Env' named entity 'cDNA' named entity 'heavy chain' named entity 'variable regions' named entity 'antibody' named entity 'light chain' named entity 'CDR3' named entity 'VDJ' named entity 'antibody' named entity 'antibody' named entity 'PCR' named entity 'covalently linked' named entity 'HIV-1' named entity 'VDJ' named entity 'antibody' named entity 'antibody' named entity 'variable regions' named entity 'primers' named entity 'Hilden' named entity 'HIV-1 infection' named entity 'infection' named entity 'gDNA' named entity 'cDNA libraries' named entity 'germline' named entity 'phagemid'

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