About: Performance of a multiplexed serological microarray for the detection of antibodies against central nervous system pathogens

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About: Performance of a multiplexed serological microarray for the detection of antibodies against central nervous system pathogens Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Performance of a multiplexed serological microarray for the detection of antibodies against central nervous system pathogens
Creator	Kurkela, Satu Bergström, Tomas Vaheri, Antti Vapalahti, Olli Hepojoki, Satu Kallio-Kokko, Hannimari Jääskeläinen, Anne Närvänen, Ale Sillanpää, Heidi Suni, Jukka Viitala, Sari
Source	Elsevier; Medline; PMC
abstract	Abstract Central nervous system (CNS) infections have multiple potential causative agents for which simultaneous pathogen screening can provide a useful tool. This study evaluated a multiplexed microarray for the simultaneous detection of antibodies against CNS pathogens. The performance of selected microarray antigens for the detection of IgG antibodies against herpes simplex virus 1 and 2 (HSV-1 and HSV-2), varicella-zoster virus (VZV), adenovirus, Mycoplasma pneumoniae and Borrelia burgdorferi sensu lato, was evaluated using serum sample panels tested with reference assays used in a routine diagnostic laboratory. The microarray sensitivity for HSV-1, HSV-2, VZV, adenovirus and M. pneumonia ranged from 77% to 100%, and the specificity ranged from 74% to 97%. Very variable sensitivities and specificities were found for borrelial antigens of three different VlsE protein IR(6) peptide variants (IR6p1, IR6p2, IR6p4) and three recombinant decorin binding proteins A (DbpA; DbpAIa, DbpA91, DbpAG40). For single antigens, good specificity was shown for antigens of IR6p4 and DbpAIa (96%), while DbpA91, IR6p1 and IR6p2 were moderately specific (88–92%). The analytical sensitivity of the microarray was dependent on the borrelial IgG concentration of the specimen. The overall performance and technical features of the platform showed that the platform supports both recombinant proteins, whole viruses and peptides as antigens. This study showed diagnostic potential for all six CNS pathogens, including Borrelia burgdorferi sensu lato, using glutaraldehyde based microarray, and further highlighted the importance of careful antigen selection and the requirement for the use of multiple borrelial antigens in order to increase specificity without a major lack of sensitivity.
has issue date	2014-05-31(xsd:dateTime)
bibo:doi	10.1016/j.mimet.2014.02.011
bibo:pmid	24594410
has license	els-covid
sha1sum (hex)	a47e947e1902cdfd8d03f1908b30b27f91fe92d7
schema:url	https://doi.org/10.1016/j.mimet.2014.02.011
resource representing a document's title	Performance of a multiplexed serological microarray for the detection of antibodies against central nervous system pathogens
has PubMed Central identifier	PMC7172757
has PubMed identifier	24594410
schema:publication	Journal of Microbiological Methods
resource representing a document's body	covid:a47e947e1902cdfd8d03f1908b30b27f91fe92d7#body_text
is schema:about of	named entity 'antigens' named entity 'concentration' named entity 'specific' named entity 'protein' named entity 'variable' named entity 'specificities' named entity 'adenovirus' named entity 'multiple' named entity 'sensitivity' named entity 'pathogens' named entity 'central nervous system' named entity 'TOOL' named entity 'BINDING PROTEINS' named entity 'FOUND' named entity 'REQUIREMENT' named entity 'SELECTED' named entity 'ANTIGEN' named entity 'HSV-2' named entity 'CNS' named entity 'For' named entity 'antibodies' named entity 'order' named entity 'laboratory' named entity 'selected' named entity 'antigens' named entity 'serological' named entity 'CNS' named entity 'HSV-2' named entity 'antigens' named entity 'study' named entity 'proteins' named entity 'assays' named entity 'potential' named entity 'selection' named entity 'microarray' named entity 'pathogen' named entity 'adenovirus' named entity 'CNS' named entity 'peptides' named entity 'VZV' named entity 'Mycoplasma pneumoniae' named entity 'M. pneumonia' named entity 'pathogens' named entity 'adenovirus' named entity 'Borrelia burgdorferi sensu lato' named entity 'protein' named entity 'DbpA' named entity 'recombinant' named entity 'antibodies' named entity 'pathogens' named entity 'immunoblot' named entity 'Borrelia burgdorferi sensu lato' named entity 'B. garinii' named entity 'serum samples' named entity 'adenovirus' named entity 'bovine serum albumin' named entity 'microarray' named entity 'pneumococcal' named entity 'horse radish' named entity 'microarray' named entity 'BSA' named entity 'microarray' named entity 'antigens' named entity 'virus' named entity 'polystyrene' named entity 'digital microscope' named entity 'B. burgdorferi' named entity 'serum samples' named entity 'serological' named entity 'immunoassays' named entity 'HSV'

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