About: Coronavirus IBV: Partial amino terminal sequencing of spike polypeptide S2 identifies the sequence Arg-Arg-Phe-Arg-Arg at the cleavage site of the spike precursor propolypeptide of IBV strains Beaudette and M41

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About: Coronavirus IBV: Partial amino terminal sequencing of spike polypeptide S2 identifies the sequence Arg-Arg-Phe-Arg-Arg at the cleavage site of the spike precursor propolypeptide of IBV strains Beaudette and M41 Goto Sponge NotDistinct Permalink

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Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Coronavirus IBV: Partial amino terminal sequencing of spike polypeptide S2 identifies the sequence Arg-Arg-Phe-Arg-Arg at the cleavage site of the spike precursor propolypeptide of IBV strains Beaudette and M41
Creator	Binns, Matthew Boursnell ', Michael Brown ' ', K Cavanagh', David Darryl, I David, T Davis, Philip Pappin, J
source	Elsevier; Medline; PMC
abstract	Abstract The spike protein of avian infectious bronchitis coronavirus comprises two glycopolypeptides S1 and S2 derived by cleavage of a proglycopolypeptide So, the nucleotide sequence of which has recently been determined for the Beaudette strain (Binns M.M. et al., 1985, J. Gen. Virol. 66, 719–726). The order of the two glycopolypeptides within So is aminoterminus(N)-Sl-S2-carboxyterminus(C). To locate the N-terminus of S2 we have performed partial amino acid sequencing on S2 from IBV-Beaudette labelled with [3H]serine and from the related strain IBV-M41 labelled with [3H]valine, leucine and isoleucine. The residues identified and their positions relative to the N-terminus of S2 were: serine, 13; valine, 6, 12; leucine, none in the first 20 residues; isoleucine, 2, 19. These results identified the N-terminus of S2 of IBV-Beaudette as serine, 520 residues from the N-terminus of S1, excluding the signal sequence. Immediately to the N-terminal side of residue 520 So has the sequence Arg-Arg-Phe-Arg-Arg; similar basic connecting peptides are a feature of several other virus spike glycoproteins. It was deduced that for IBV-Beaudette SI comprises 519 residues (M r 57.0K) or 514 residues (56.2K) if the connecting peptide was to be removed by carboxypeptidase-like activity in vivo while S2 has 625 residues (69.2K). Nucleotide sequencing of the cleavage region of the So gene of IBV-M41 revealed the same connecting peptide as IBV-Beaudette and that the first 20 N-terminal residues of S2 of IBV-M41 were identical to those of the Beaudette strain. IBV-Beaudette grown in Vero cells had some uncleaved So; this was cleavable by 10 μg/ml of trypsin and of chymotrypsin. Partial N-terminal analysis of S1 from IBV-M41 identified leucine and valine residues at positions 2 and 9 respectively from the N-terminus. This confirms the identification made by Binns et al. (1985), of the N-terminus of S1 and the end of the signal sequence of the IBV-Beaudette spike propolypeptide. N-terminal sequencing of [3H]leucine-labelled IBV-Beaudette membrane (M) polypeptide showed leucine residues at positions 8,16 and 22 from the N-terminus; these results confirm the open reading frame identified by M.E.G. Boursnell et al. (1984, Virus Res. 1, 303–313) in the nucleotide sequence of M. The N-terminus of the nucleocapsid (n) polypeptide appeared to be blocked.
has issue date	1986-02-28(xsd:dateTime)
bibo:doi	10.1016/0168-1702(86)90037-7
bibo:pmid	3010595
has license	els-covid
sha1sum (hex)	a4a0da27f4a97187a30636811ce80076c7ea23b1
schema:url	https://doi.org/10.1016/0168-1702%2886%2990037-7
resource representing a document's title	Coronavirus IBV: Partial amino terminal sequencing of spike polypeptide S2 identifies the sequence Arg-Arg-Phe-Arg-Arg at the cleavage site of the spike precursor propolypeptide of IBV strains Beaudette and M41
has PubMed Central identifier	PMC7133853
has PubMed identifier	3010595
schema:publication	Virus Research
resource representing a document's body	covid:a4a0da27f4a97187a30636811ce80076c7ea23b1#body_text
is schema:about of	named entity 'Coronavirus' named entity 'sequence' named entity 'PHE-ARG-ARG' named entity 'NUCLEOTIDE SEQUENCE' named entity 'CORONAVIRUS' named entity 'VIROL' named entity 'CLEAVAGE' named entity 'ARG-ARG' named entity 'AMINO TERMINAL' named entity 'POLYPEPTIDE' named entity 'RECENTLY' named entity 'SEQUENCING' named entity 'CORONAVIRUS' named entity 'PARTIAL' named entity 'SITE OF' named entity 'SEQUENCE' named entity 'PRECURSOR' named entity 'SPIKE' named entity 'CLEAVAGE' named entity 'IBV' named entity 'DERIVED' named entity 'STRAINS' named entity 'DETERMINED' named entity 'M41' named entity 'ARG-PHE' named entity 'SPIKE PROTEIN' named entity 'GEN' named entity '726' named entity 'STRAIN' named entity 'AVIAN INFECTIOUS BRONCHITIS' named entity 'PROTEIN ' covid:arg/a4a0da27f4a97187a30636811ce80076c7ea23b1 named entity 'Binns' named entity 'coronavirus' named entity '1985' named entity 'nucleotide sequence' named entity 'amino terminal' named entity 'avian infectious bronchitis' named entity 'spike protein' named entity 'Phe' named entity 'Arg' named entity 'Arg' named entity 'IBV' named entity 'signal peptide' named entity 'Vero cells' named entity 'Trypsin' named entity 'single-stranded' named entity 'cleavage' named entity 'hybridisation' named entity 'enzyme' named entity 'N-terminus' named entity 'cleavage' named entity 'amino terminus' named entity 'TPCK' named entity 'trypsin' named entity 'valine' named entity 'virus' named entity 'serine' named entity 'kidney cells' named entity 'moiety' named entity 'retroviruses' named entity 'cleavage site' named entity 'N-terminal' named entity 'nucleocapsid protein' named entity 'hydrophobic' named entity 'Amino acid sequencing' named entity 'polypeptide' named entity 'valine' named entity 'N-terminal sequencing' named entity 'primer' named entity 'signal sequence' named entity 'scintillation cocktail' named entity 'signal sequence' named entity 'proproteins'

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