About: Comparison of two commercial molecular assays for simultaneous detection of respiratory viruses in clinical samples using two automatic electrophoresis detection systems

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About: Comparison of two commercial molecular assays for simultaneous detection of respiratory viruses in clinical samples using two automatic electrophoresis detection systems Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Comparison of two commercial molecular assays for simultaneous detection of respiratory viruses in clinical samples using two automatic electrophoresis detection systems
Creator	Wallinga, J Bruijnesteijn Van Coppenraet, L Nijhuis, R Ruijs, G Schirm, J Swanink, C Van Zwet, A
topic	covid:a4ff9e9c572c22e93adcb8845c68f6dab985717b#this
Source	Elsevier; Medline; PMC
abstract	Two molecular assays were compared with real-time RT-PCR and viral culture for simultaneous detection of common viruses from respiratory samples: a multiplex ligation-dependant probe amplification (MLPA) and a dual priming oligonucleotide system (DPO). In addition, the positive detections of MLPA and DPO were identified using two different automatic electrophoresis systems. A panel of 168 culture-positive and negative samples was tested by the molecular assays for the presence of influenza A and B virus, respiratory syncytial virus, human metapneumovirus, rhinovirus, coronaviruses, parainfluenza viruses and adenovirus. One hundred and twenty-nine (77%) samples were positive as detected by at least one method. Sixty-nine (41%) samples were positive by cell culture (excluding human metapneumovirus and coronaviruses), 116 (69%) by RT-PCR, 127 (76%) by MLPA and 100 (60%) by DPO. The MLPA yielded results in one attempt for all samples included while 12 (7.2%) samples had to be repeated by the DPO assay due to inconclusive results. The MLPA assay performed well in combination with either electrophoresis system, while the performance of the DPO assay was influenced by the electrophoresis systems. Both molecular assays are comparable with real-time RT-PCR, more sensitive than viral culture and can detect dual infections easily. Results can be obtained within 1 day.
has issue date	2010-08-05(xsd:dateTime)
bibo:doi	10.1016/j.jviromet.2010.07.032
bibo:pmid	20691735
has license	no-cc
sha1sum (hex)	a4ff9e9c572c22e93adcb8845c68f6dab985717b
schema:url	https://doi.org/10.1016/j.jviromet.2010.07.032
resource representing a document's title	Comparison of two commercial molecular assays for simultaneous detection of respiratory viruses in clinical samples using two automatic electrophoresis detection systems
has PubMed Central identifier	PMC7119677
has PubMed identifier	20691735
schema:publication	J Virol Methods
resource representing a document's body	covid:a4ff9e9c572c22e93adcb8845c68f6dab985717b#body_text
is http://vocab.deri.ie/void#inDataset of	proxy:http/ns.inria.fr/covid19/a4ff9e9c572c22e93adcb8845c68f6dab985717b
is schema:about of	named entity 'probe' named entity 'systems' named entity 'SAMPLES' named entity 'COMMERCIAL' named entity 'MOLECULAR' named entity 'CLINICAL' named entity 'RESPIRATORY VIRUSES' named entity 'TESTED' named entity 'DPO' named entity 'RHINOVIRUS' named entity 'SYSTEM' named entity 'PARAINFLUENZA' named entity 'CORONAVIRUSES' named entity 'SIMULTANEOUS' named entity 'POSITIVE' named entity 'DUAL' named entity 'PRESENCE OF' named entity 'RESPIRATORY SYNCYTIAL VIRUS' named entity 'COMPARED' named entity 'COMMON' named entity 'INFLUENZA A AND B VIRUS' named entity 'CULTURE' named entity 'HUMAN METAPNEUMOVIRUS' named entity 'AUTOMATIC' named entity 'PRIMING' named entity 'LIGATION' named entity 'AMPLIFICATION' named entity 'PROBE' named entity 'MULTIPLEX' named entity 'POSITIVE AND NEGATIVE' named entity 'DIFFERENT' named entity 'OLIGONUCLEOTIDE' named entity 'ADDITION' named entity '168' named entity 'AUTOMATIC' named entity 'USING' named entity 'DETECTION' named entity 'SIMULTANEOUS' named entity 'COMPARISON' named entity 'DEPENDANT' named entity 'SYSTEMS' named entity 'PANEL' named entity 'VIRUSES' named entity 'IDENTIFIED' named entity 'RESPIRATORY SAMPLES' named entity 'SAMPLES' named entity 'MLPA' named entity 'ELECTROPHORESIS' named entity 'MOLECULAR' named entity 'DETECTION' named entity 'ELECTROPHORESIS' named entity 'SYSTEMS' named entity 'VIRAL CULTURE' named entity 'USING' named entity 'REAL-TIME RT-PCR' named entity 'ADENOVIRUS' covid:arg/a4ff9e9c572c22e93adcb8845c68f6dab985717b named entity 'infection' named entity 'fibroblast' named entity 'Ligation' named entity 'real-time' named entity 'virus' named entity 'viruses' named entity 'assays' named entity 'MLPA' named entity 'virus' named entity 'viruses' named entity 'MLPA' named entity 'oligonucleotide' named entity 'parainfluenza' named entity 'detection systems' named entity 'assays' named entity 'respiratory syncytial virus, human'

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