About: Genomic and Proteomic Analysis of Schizaphis graminum Reveals Cyclophilin Proteins Are Involved in the Transmission of Cereal Yellow Dwarf Virus

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About: Genomic and Proteomic Analysis of Schizaphis graminum Reveals Cyclophilin Proteins Are Involved in the Transmission of Cereal Yellow Dwarf Virus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Genomic and Proteomic Analysis of Schizaphis graminum Reveals Cyclophilin Proteins Are Involved in the Transmission of Cereal Yellow Dwarf Virus
Creator	Smith, D Bereman, M Deblasio, S Igwe, D Tamborindeguy, C
Source	Medline; PMC
abstract	Yellow dwarf viruses cause the most economically important virus diseases of cereal crops worldwide and are transmitted by aphid vectors. The identification of aphid genes and proteins mediating virus transmission is critical to develop agriculturally sustainable virus management practices and to understand viral strategies for circulative movement in all insect vectors. Two cyclophilin B proteins, S28 and S29, were identified previously in populations of Schizaphisgraminum that differed in their ability to transmit the RPV strain of Cereal yellow dwarf virus (CYDV-RPV). The presence of S29 was correlated with F2 genotypes that were efficient virus transmitters. The present study revealed the two proteins were isoforms, and a single amino acid change distinguished S28 and S29. The distribution of the two alleles was determined in 12 F2 genotypes segregating for CYDV-RPV transmission capacity and in 11 genetically independent, field-collected S . graminum biotypes. Transmission efficiency for CYDV-RPV was determined in all genotypes and biotypes. The S29 isoform was present in all genotypes or biotypes that efficiently transmit CYDV-RPV and more specifically in genotypes that efficiently transport virus across the hindgut. We confirmed a direct interaction between CYDV-RPV and both S28 and S29 using purified virus and bacterially expressed, his-tagged S28 and S29 proteins. Importantly, S29 failed to interact with a closely related virus that is transported across the aphid midgut. We tested for in vivo interactions using an aphid-virus co-immunoprecipitation strategy coupled with a bottom-up LC-MS/MS analysis using a Q Exactive mass spectrometer. This analysis enabled us to identify a third cyclophilin protein, cyclophilin A, interacting directly or in complex with purified CYDV-RPV. Taken together, these data provide evidence that both cyclophilin A and B interact with CYDV-RPV, and these interactions may be important but not sufficient to mediate virus transport from the hindgut lumen into the hemocoel.
has issue date	2013-08-09(xsd:dateTime)
bibo:doi	10.1371/journal.pone.0071620
bibo:pmid	23951206
has license	cc0
sha1sum (hex)	a50f42269649e20ec3580496c72923ef1c4abd6a
schema:url	https://doi.org/10.1371/journal.pone.0071620
resource representing a document's title	Genomic and Proteomic Analysis of Schizaphis graminum Reveals Cyclophilin Proteins Are Involved in the Transmission of Cereal Yellow Dwarf Virus
has PubMed Central identifier	PMC3739738
has PubMed identifier	23951206
schema:publication	PLoS One
resource representing a document's body	covid:a50f42269649e20ec3580496c72923ef1c4abd6a#body_text
is schema:about of	named entity 'REVEALS' named entity 'CYCLOPHILIN' named entity 'proteins' named entity 'transport' named entity 'transmission' named entity 'provide' named entity 'lumen' named entity 'management' named entity 'Cereal' named entity 'PROTEOMIC ANALYSIS' named entity 'PROTEINS' named entity 'SUSTAINABLE' named entity 'MANAGEMENT PRACTICES' named entity 'DATA' named entity 'AMINO ACID CHANGE' named entity 'MASS SPECTROMETER' named entity 'INSECT VECTORS' named entity 'CORRELATED' named entity 'HINDGUT LUMEN' named entity 'IDENTIFIED' named entity 'INTERACTIONS' named entity 'UNDERSTAND' named entity 'SUFFICIENT' named entity 'RELATED' named entity 'MOVEMENT' named entity 'CO-IMMUNOPRECIPITATION' named entity 'REVEALED' named entity 'APHID' named entity 'CEREAL' named entity 'TRANSMISSION' named entity 'ISOFORM' named entity 'MAY BE' named entity 'COLLECTED' named entity 'MIDGUT' named entity 'ANALYSIS' named entity 'TO INTERACT WITH' named entity 'THEIR' named entity 'EXPRESSED' named entity 'GENES' named entity 'MEDIATE' named entity 'INDEPENDENT' named entity 'TRANSMITTED BY' named entity 'PROTEIN' named entity 'DETERMINED' named entity 'STRATEGY' named entity 'WORLDWIDE' named entity 'CONFIRMED' named entity 'FAILED' named entity 'DIRECT INTERACTION' named entity 'EFFICIENCY' named entity 'VIRUSES' named entity 'STRAIN' named entity 'CYCLOPHILIN A' named entity 'STUDY' named entity 'VIRAL' named entity 'IMPORTANT' named entity 'CAPACITY' named entity 'EVIDENCE' named entity 'TESTED FOR' named entity 'CEREAL' named entity 'GENOMIC' named entity 'CEREAL CROPS' named entity 'CYCLOPHILIN' named entity 'S29' named entity 'STRATEGIES' named entity 'IN VIVO' named entity 'COUPLED' named entity 'CRITICAL' named entity 'VECTORS' named entity 'VIRUS DISEASES' named entity 'TO IDENTIFY' named entity 'FIELD' named entity 'INVOLVED' named entity 'TRANSMISSION' named entity 'SCHIZAPHIS GRAMINUM' named entity 'VIRUS' named entity 'YELLOW DWARF'

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