About: Novel Approach for Isolation and Identification of Porcine Epidemic Diarrhea Virus (PEDV) Strain NJ Using Porcine Intestinal Epithelial Cells

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About: Novel Approach for Isolation and Identification of Porcine Epidemic Diarrhea Virus (PEDV) Strain NJ Using Porcine Intestinal Epithelial Cells Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Novel Approach for Isolation and Identification of Porcine Epidemic Diarrhea Virus (PEDV) Strain NJ Using Porcine Intestinal Epithelial Cells
Creator	Wu, Yang Chen, Ying Li, Yijing Xu, Yigang Ma, Sunting Wang, Xiaona Yu, Meiling Shi, Wen Dixon, Linda Fan, Wenlu Jia, Shuo Yin, Jiyuan Zhao, Haiyuan Graham, Simon
Source	Medline; PMC
abstract	Porcine epidemic diarrhea virus (PEDV), which is the causative agent of porcine epidemic diarrhea in China and other countries, is responsible for serious economic losses in the pork industry. Inactivated PEDV vaccine plays a key role in controlling the prevalence of PEDV. However, consistently low viral titers are obtained during the propagation of PEDV in vitro; this represents a challenge to molecular analyses of the virus and vaccine development. In this study, we successfully isolated a PEDV isolate (strain NJ) from clinical samples collected during a recent outbreak of diarrhea in piglets in China, using porcine intestinal epithelial cells (IEC). We found that the isolate was better adapted to growth in IECs than in Vero cells, and the titer of the IEC cultures was 10(4.5) TCID(50)/0.1 mL at passage 45. Mutations in the S protein increased with the viral passage and the mutations tended towards attenuation. Viral challenge showed that the survival of IEC-adapted cultures was higher at the 45th passage than at the 5th passage. The use of IECs to isolate and propagate PEDV provides an effective approach for laboratory-based diagnosis of PEDV, as well as studies of the epidemiological characteristics and molecular biology of this virus.
has issue date	2017-01-21(xsd:dateTime)
bibo:doi	10.3390/v9010019
bibo:pmid	28117718
has license	cc-by
sha1sum (hex)	a53b809c3b22d019046ee761e05c464bd629079a
schema:url	https://doi.org/10.3390/v9010019
resource representing a document's title	Novel Approach for Isolation and Identification of Porcine Epidemic Diarrhea Virus (PEDV) Strain NJ Using Porcine Intestinal Epithelial Cells
has PubMed Central identifier	PMC5294988
has PubMed identifier	28117718
schema:publication	Viruses
resource representing a document's body	covid:a53b809c3b22d019046ee761e05c464bd629079a#body_text
is schema:about of	named entity 'attenuation' named entity 'biology' named entity 'development' named entity 'Porcine' named entity 'STRAIN' named entity 'NOVEL' named entity 'USE OF' named entity 'TCID' named entity 'PORCINE' named entity 'CHINA' named entity 'STUDY' named entity 'BASED' named entity 'TITER' named entity 'INTESTINAL' named entity 'S PROTEIN' named entity 'CONTROLLING' named entity 'MOLECULAR BIOLOGY' named entity 'MOLECULAR' named entity 'GROWTH' named entity 'CLINICAL' named entity 'OBTAINED' named entity '5TH' named entity 'OUTBREAK' named entity 'VIRUS' named entity 'APPROACH' named entity 'PROTEIN ' named entity 'PORCINE EPIDEMIC DIARRHEA VIRUS' named entity 'KEY' named entity 'PROPAGATION' named entity 'BETTER' named entity 'HIGHER' named entity 'PREVALENCE' named entity 'USING' named entity 'PLAYS' named entity 'VACCINE' named entity 'ISOLATE' named entity 'CHALLENGE' named entity 'VIRAL' named entity 'SAMPLES' named entity 'COUNTRIES' named entity 'INDUSTRY' named entity 'ROLE' named entity 'COLLECTED' named entity 'DIARRHEA' named entity '4.5' covid:arg/a53b809c3b22d019046ee761e05c464bd629079a named entity 'INTESTINAL' named entity 'EPIDEMIOLOGICAL' named entity 'ISOLATION' named entity 'PORCINE' named entity 'USING' named entity 'CULTURES' named entity 'STUDIES' named entity 'EPITHELIAL CELLS' named entity 'IDENTIFICATION' named entity 'PORCINE EPIDEMIC DIARRHEA VIRUS' named entity 'APPROACH' named entity 'IEC' named entity 'INCREASED' named entity 'LABORATORY' named entity 'PORCINE EPIDEMIC DIARRHEA' named entity 'IN VITRO' named entity 'VERO CELLS' named entity 'ATTENUATION' named entity 'PORK' named entity 'ADAPTED' named entity 'DIAGNOSIS' named entity 'MUTATIONS'

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