About: Characterization of ubiquitin and ubiquitin-like-protein isopeptidase activities

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About: Characterization of ubiquitin and ubiquitin-like-protein isopeptidase activities Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Characterization of ubiquitin and ubiquitin-like-protein isopeptidase activities
Creator	Leach, Craig Nicholson, Benjamin Tian, Xufan Bernal, Alejandro Shanks, John Mattern, Michael Wilkinson, Keith Butt, Tauseef Francis, Dana Goldenberg, Seth Kodrasov, Matthew Sterner, David
Source	Medline; PMC
abstract	Conjugation or deconjugation of ubiquitin (Ub) or ubiquitin-like proteins (UBLs) to or from cellular proteins is a multifaceted and universal means of regulating cellular physiology, controlling the lifetime, localization, and activity of many critical proteins. Deconjugation of Ub or UBL from proteins is performed by a class of proteases called isopeptidases. Herein is described a readily quantifiable novel isopeptidase assay platform consisting of Ub or UBL fused to the reporter enzyme phospholipase A(2) (PLA(2)). Isopeptidase activity releases PLA(2), which cleaves its substrate, generating a signal that is linear with deubiquitylase (DUB) concentration and is able to discriminate DUB, deSUMOylase, deNEDDylase, and deISGylase activities. The power and sensitivity of the UBL-PLA(2) assay are demonstrated by its ability to differentiate the contrasting deISGylase and DUB activities of two coronavirus proteases: severe acute respiratory syndrome papain-like protease (SARS-CoV PLpro) and NL63 CoV papain-like protease 2 (PLP2). Furthermore, direct comparisons with the current Ub-7-amino-4-methylcoumarin (Ub-AMC) assay demonstrated that the Ub-PLA(2) assay is an effective tool for characterizing modulators of isopeptidase activity. This observation was expanded by profiling the inhibitory activity of the nonselective isopeptidase inhibitor NSC 632839 against DUBs and deSUMOylases. Taken together, these studies illustrate the utility of the reporter-based approach to measuring isopeptidase activity.
has issue date	2008-06-01(xsd:dateTime)
bibo:doi	10.1110/ps.083450408
bibo:pmid	18424514
has license	green-oa
sha1sum (hex)	a61e5889417eb9962a005eadfba3c9d28e73a9af
schema:url	https://doi.org/10.1110/ps.083450408
resource representing a document's title	Characterization of ubiquitin and ubiquitin-like-protein isopeptidase activities
has PubMed Central identifier	PMC2386736
has PubMed identifier	18424514
schema:publication	Protein Science
resource representing a document's body	covid:a61e5889417eb9962a005eadfba3c9d28e73a9af#body_text
is schema:about of	named entity 'called' named entity 'proteases' named entity 'power' named entity 'This' named entity 'activities' named entity 'severe acute respiratory syndrome' named entity 'DUB' named entity 'ACTIVITIES' named entity 'ISOPEPTIDASE' named entity 'PROTEASES' named entity 'TOOL' named entity 'THESE' named entity 'PROTEASE 2' named entity 'QUANTIFIABLE' named entity 'REGULATING' named entity 'NSC 632839' named entity 'REPORTER' named entity 'INHIBITOR' named entity 'MEASURING' named entity 'LIKE' named entity 'DISCRIMINATE' named entity 'REPORTER ENZYME' named entity 'MODULATORS' named entity 'FUSED TO' named entity 'CRITICAL' named entity 'A CLASS' named entity 'PROTEASE' named entity 'DESCRIBED' named entity 'CHARACTERIZATION' named entity 'UBIQUITIN' named entity 'LIKE' named entity 'PROTEIN ' named entity 'CURRENT' named entity 'SENSITIVITY' named entity 'PERFORMED BY' named entity 'DIFFERENTIATE' named entity 'DEUBIQUITYLASE' named entity 'SEVERE ACUTE RESPIRATORY SYNDROME' named entity 'GENERATING' named entity 'PAPAIN' named entity 'CONTRASTING' named entity 'SIGNAL' named entity 'CELLULAR PHYSIOLOGY' named entity 'MEANS' named entity 'BASED' named entity 'CORONAVIRUS' named entity 'MULTIFACETED' named entity 'UNIVERSAL' named entity 'CONCENTRATION' named entity 'CLEAVES' named entity 'STUDIES' named entity 'LIFETIME' named entity 'ISOPEPTIDASE' named entity 'ITS' named entity 'ACTIVITIES' named entity 'SUBSTRATE' named entity 'PHOSPHOLIPASE A 2' named entity 'OBSERVATION' named entity 'IS A' named entity 'PLA' named entity 'DEMONSTRATED' named entity 'NOVEL' named entity 'RELEASES' named entity 'CONJUGATION' named entity '7-AMINO-4-METHYLCOUMARIN' named entity 'APPROACH' named entity 'UBL' named entity 'TAKEN' named entity 'POWER' named entity 'CONTROLLING'

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