About: A Real-Time PCR Assay for Bat SARS-Like Coronavirus Detection and Its Application to Italian Greater Horseshoe Bat Faecal Sample Surveys

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About: A Real-Time PCR Assay for Bat SARS-Like Coronavirus Detection and Its Application to Italian Greater Horseshoe Bat Faecal Sample Surveys Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	A Real-Time PCR Assay for Bat SARS-Like Coronavirus Detection and Its Application to Italian Greater Horseshoe Bat Faecal Sample Surveys
Creator	Battilani, Mara Balboni, Andrea Gallina, Laura Palladini, Alessandra Prosperi, Santino Marquet, R Mccrae, M Morozov, S
source	Medline; PMC
abstract	Bats are source of coronaviruses closely related to the severe acute respiratory syndrome (SARS) virus. Numerous studies have been carried out to identify new bat viruses related to SARS-coronavirus (bat-SARS-like CoVs) using a reverse-transcribed-polymerase chain reaction assay. However, a qualitative PCR could underestimate the prevalence of infection, affecting the epidemiological evaluation of bats in viral ecology. In this work an SYBR Green-real time PCR assay was developed for diagnosing infection with SARS-related coronaviruses from bat guano and was applied as screening tool in a survey carried out on 45 greater horseshoe bats (Rhinolophus ferrumequinum) sampled in Italy in 2009. The assay showed high sensitivity and reproducibility. Its application on bats screening resulted in a prevalence of 42%. This method could be suitable as screening tool in epidemiological surveys about the presence of bat-SARS-like CoVs, consequently to obtain a more realistic scenario of the viral prevalence in the population.
has issue date	2011-11-22(xsd:dateTime)
bibo:doi	10.1100/2012/989514
bibo:pmid	22654650
has license	cc-by
sha1sum (hex)	a64792baacb18e3bbfb88eee3a3592c85f04a627
schema:url	https://doi.org/10.1100/2012/989514
resource representing a document's title	A Real-Time PCR Assay for Bat SARS-Like Coronavirus Detection and Its Application to Italian Greater Horseshoe Bat Faecal Sample Surveys
has PubMed Central identifier	PMC3353321
has PubMed identifier	22654650
schema:publication	ScientificWorldJournal
resource representing a document's body	covid:a64792baacb18e3bbfb88eee3a3592c85f04a627#body_text
is schema:about of	named entity 'PCR ASSAY' named entity 'DETECTION' named entity '2009' named entity 'However' named entity 'tool' named entity 'prevalence' named entity 'coronaviruses' named entity 'Application' named entity 'Greater Horseshoe Bat' named entity 'APPLICATION' covid:arg/a64792baacb18e3bbfb88eee3a3592c85f04a627 named entity 'REVERSE' named entity 'VIRUSES' named entity 'SYBR GREEN' named entity 'APPLIED' named entity 'TOOL' named entity 'SUITABLE' named entity 'SAMPLED' named entity 'DIAGNOSING INFECTION' named entity 'AFFECTING' named entity 'GUANO' named entity 'CARRIED' named entity 'PREVALENCE' named entity 'EVALUATION' named entity 'ITS' named entity 'POPULATION' named entity 'COVS' named entity 'PRESENCE OF' named entity 'NUMEROUS' named entity 'LIKE' named entity 'COULD BE' named entity 'SURVEY' named entity 'TRANSCRIBED' named entity 'NEW' named entity 'TO IDENTIFY' named entity 'HORSESHOE BATS' named entity 'INFECTION' named entity 'EPIDEMIOLOGICAL' named entity 'SARS' named entity 'APPLICATION' named entity 'METHOD' named entity 'SURVEYS' named entity 'SOURCE' named entity 'REPRODUCIBILITY' named entity 'POLYMERASE CHAIN REACTION ASSAY' named entity 'CORONAVIRUSES' named entity 'ABOUT' named entity 'RELATED' named entity 'SCENARIO' named entity 'USING' named entity 'ASSAY' named entity 'SEVERE ACUTE RESPIRATORY SYNDROME' named entity 'ECOLOGY' named entity 'STUDIES' named entity 'CORONAVIRUS' named entity 'SCREENING' named entity 'HIGH SENSITIVITY' named entity 'PCR' named entity 'SURVEYS' named entity 'SAMPLE' named entity 'ITALIAN' named entity 'BAT SARS-LIKE CORONAVIRUS' named entity 'REAL-TIME PCR ' named entity 'GREATER HORSESHOE BAT' named entity 'FAECAL' named entity 'VIRUS' named entity 'ITALY' named entity 'VIRAL' named entity 'QUALITATIVE' named entity 'BATS' named entity 'GREATER' named entity 'ITS' named entity 'PCR ASSAY' named entity 'OBTAIN'

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