About: Strain-specific transcriptional and posttranscriptional regulation of heat-labile toxin expression by enterotoxigenic Escherichia coli

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About: Strain-specific transcriptional and posttranscriptional regulation of heat-labile toxin expression by enterotoxigenic Escherichia coli Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Strain-specific transcriptional and posttranscriptional regulation of heat-labile toxin expression by enterotoxigenic Escherichia coli
Creator	Luís, & De, Mariana Mathias-Santos, Camila Nakao, Naomi Rodrigues, Juliana Barros Luiz, Wilson Br, Lcsf@usp Cintra, Jesus De, Carlos Fabris Maeda, Nascimento Ferreira Lourenço, Rogério Ferreira, Souza Lina, Denicar Luís, *
Source	PMC
abstract	Enterotoxigenic Escherichia coli (ETEC) represents one of the most important etiological agents of diarrhea in developing countries and characteristically produces at least one of two enterotoxins: heat-labile toxin (LT) and heat-stable toxin (ST). It has been previously shown that the production and release of LT by human-derived ETEC strains are variable. Although the natural genetic polymorphisms of regulatory sequences of LT-encoding (eltAB) genes may explain the variable production of LT, the knowledge of the transcriptional and posttranscriptional aspects affecting LT expression among ETEC strains is not clear. To further understand the factors affecting LT expression, we evaluated the impact of the natural polymorphism in noncoding regulatory sequences of eltAB among clinically derived ETEC strains. Sequence analyses of seven clinically derived strains and the reference strain H10407 revealed polymorphic sites at both the promoter and upstream regions of the eltAB operon. Operon fusion assays with GFP revealed that specific nucleotide changes in the Pribnow box reduce eltAB transcription. Nonetheless, the total amounts of LT produced by the tested ETEC strains did not strictly correspond to the detected LT-specific mRNA levels. Indeed, the stability of LT varied according to the tested strain, indicating the presence of posttranscriptional mechanisms affecting LT expression. Taken together, our results indicate that the production of LT is a strain-specific process and involves transcriptional and posttranscriptional mechanisms that regulate the final amount of toxin produced and released by specific strains. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s42770-020-00231-2) contains supplementary material, which is available to authorized users.
has issue date	2020-02-03(xsd:dateTime)
bibo:doi	10.1007/s42770-020-00231-2
bibo:pmid	32016818
has license	no-cc
sha1sum (hex)	a684887ce8435cf8345530c93156623bc202070d
schema:url	https://doi.org/10.1007/s42770-020-00231-2
resource representing a document's title	Strain-specific transcriptional and posttranscriptional regulation of heat-labile toxin expression by enterotoxigenic Escherichia coli
has PubMed Central identifier	PMC7203263
has PubMed identifier	32016818
schema:publication	Braz J Microbiol
resource representing a document's body	covid:a684887ce8435cf8345530c93156623bc202070d#body_text
is schema:about of	named entity 'presence' named entity 'evaluated' named entity 'mechanisms' named entity 'expression' named entity 'total' named entity 'etiological' named entity 'Enterotoxigenic Escherichia coli' named entity 'production' named entity 'transcriptional' named entity 'TRANSCRIPTIONAL' named entity 'ENCODING' named entity 'AFFECTING' named entity 'PRIBNOW BOX' named entity 'CHANGES' named entity 'REDUCE' named entity 'INDICATING' named entity 'natural' named entity 'Although' named entity 'stability' named entity 'strains' named entity 'expression' named entity 'polymorphism' named entity 'eltAB' named entity 'eltAB' named entity 'diarrhea' named entity 'toxin' named entity 'regulatory sequences' named entity 'operon' named entity 'posttranscriptional' named entity 'Enterotoxigenic Escherichia coli' named entity 'GFP' named entity 'nucleotide' named entity 'posttranscriptional' named entity 'posttranscriptional' named entity 'transcriptional' named entity 'Primer' named entity 'eltAB' named entity 'ETEC' named entity 'ANOVA' named entity 'heterogeneity' named entity 'PCR' named entity 'eltAB' named entity 'eltAB' named entity 'ETEC' named entity 'toxin' named entity 'therapeutic approaches' named entity 'CRP' named entity 'total protein' named entity 'Bradford assay' named entity 'rooted tree' named entity 'G-14' named entity 'nucleotides' named entity '0.01' named entity 'GFP' named entity 'eltAB' named entity 'eltAB' named entity 'eltAB' named entity 'phylogenetically related' named entity 'saline' named entity 'GenBank' named entity 'eltAB' named entity 'C-7' named entity 'toxin' named entity 'primers' named entity 'watery diarrhea' named entity 'total protein' named entity 'cDNA' named entity 'E. coli'

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