About: Immunogenicity and protective efficacy of orally administered recombinant Lactobacillus plantarum expressing VP2 protein against IBDV in chicken

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About: Immunogenicity and protective efficacy of orally administered recombinant Lactobacillus plantarum expressing VP2 protein against IBDV in chicken Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Immunogenicity and protective efficacy of orally administered recombinant Lactobacillus plantarum expressing VP2 protein against IBDV in chicken
Creator	Shi, Wen Wang, L Wang, X Li, Y Han, B Shi, W Xu, Y Zhao, H Jamal, S Tang, L Din, M Maqsood, I Maqsood, Iram Moshin, B Nadeem, A Saima, K
source	Medline; PMC
abstract	AIM: To develop an effective oral vaccine against the very virulent infectious bursal disease virus (vvIBDV), we generated two recombinant Lactobacillus plantarum strains (pPG612‐VP2/LP and pPG612‐T7g10‐VP2/LP, which carried the T7g10 translational enhancer) that displayed the VP2 protein on the surface, and compared the humoral and cellular immune responses against vvIBDV in chickens. METHODS AND RESULTS: We genetically engineered the L. plantarum strains pPG612‐VP2/LP and pPG612‐T7g10‐VP2/LP constitutively expressing the VP2 protein of vvIBDV. We found that the T7g10 enhancer efficiently upregulates VP2 expression in pPG612‐T7g10‐VP2/LP. Orally administered, pPG612‐T7g10‐VP2/LP exhibited significant levels of protection (87·5%) against vvIBDV in chickens, indicating improved immunogenicity. Chickens in the pPG612‐T7g10‐VP2/LP group produced higher levels of interferons (IFN‐γ) and interleukins (IL‐2 and IL‐4) than those in the pPG612‐VP2/LP group. CD8+ and CD4+ lymphocyte counts indicated greater stimulation in the pPG612‐T7g10‐VP2/LP group (13·3 and 21·0% respectively) than in the pPG612‐VP2/LP group (10·4 and 14·0% respectively). Thus, pPG612‐T7g10‐VP2/LP could induce strong humoral and cellular immune responses against vvIBDV. CONCLUSIONS: The recombinant L. plantarum that expresses pPG612‐T7g10‐VP2 is a promising candidate for oral vaccine development against vvIBDV. SIGNIFICANCE AND IMPACT OF THE STUDY: The recombinant Lactobacillus delivery system provides a promising strategy for vaccine development against vvIBDV in chickens.
has issue date	2018-09-27(xsd:dateTime)
bibo:doi	10.1111/jam.14073
bibo:pmid	30118165
has license	no-cc
sha1sum (hex)	a6b4e2dd6ed077a4df8600ef70834dbe197895c1
schema:url	https://doi.org/10.1111/jam.14073
resource representing a document's title	Immunogenicity and protective efficacy of orally administered recombinant Lactobacillus plantarum expressing VP2 protein against IBDV in chicken
has PubMed Central identifier	PMC7166448
has PubMed identifier	30118165
schema:publication	J Appl Microbiol
resource representing a document's body	covid:a6b4e2dd6ed077a4df8600ef70834dbe197895c1#body_text
is schema:about of	named entity 'cellular immune responses' named entity 'generated' named entity 'Chickens' named entity 'develop' named entity 'VP2' named entity 'SIGNIFICANCE' named entity 'DISPLAYED' named entity 'ORALLY' named entity '2887' named entity 'LYMPHOCYTE COUNTS' named entity 'vaccine' named entity 'chickens' named entity 'group' named entity 'greater' named entity 'recombinant' named entity 'humoral' named entity 'strains' named entity 'carried' named entity 'expression' named entity 'genetically engineered' named entity 'enhancer' named entity 'vaccine' named entity 'oral vaccine' named entity 'Lactobacillus plantarum' named entity 'L. plantarum' named entity 'recombinant' named entity 'L. plantarum' named entity 'CD8+' named entity 'Immunogenicity' named entity 'VP2' named entity 'virally infected' named entity 'VP2' named entity 'SDS-PAGE' named entity 'apoptosis' named entity 'Invitrogen' named entity 'genus' named entity 'atrophy' named entity 'epithelial' named entity 'antibodies' named entity 'IL-2' named entity 'VP2' named entity 'Th2' named entity 'gene' named entity 'PBS' named entity 'gene expression' named entity 'cytokine' named entity 'skimmed milk' named entity 'IBDV' named entity 'antiseptic' named entity 'VP2' named entity 'RNA-dependent RNA polymerase' named entity 'IBDV' named entity 'virulent' named entity 'IBDV' named entity 'immunogenicity' named entity 'DB11' named entity 'L. plantarum' named entity 'recombinant vaccines' named entity 'Lactobacillus' named entity 'VP2' named entity 'DB11' named entity 'optical density' named entity 'VP2' named entity 'L. plantarum' named entity 'VP2' named entity 'serum'

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