About: Significant inhibition of re-emerged and emerging swine enteric coronavirus in vitro using the multiple shRNA expression vector

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About: Significant inhibition of re-emerged and emerging swine enteric coronavirus in vitro using the multiple shRNA expression vector Goto Sponge NotDistinct Permalink

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Significant inhibition of re-emerged and emerging swine enteric coronavirus in vitro using the multiple shRNA expression vector
Creator	Li, Kai Ye, Yu Huang, Dongyan Song, Deping Tang, Yuxin Zhang, Fanfan Bi, Zhen Gong, Wang Li, Hao Luo, Suxian Li, Zhiquan Lei, Dan
Source	Elsevier; Medline; PMC
abstract	Abstract Swine enteric coronaviruses (SECoVs), including porcine epidemic diarrhea virus (PEDV), swine acute diarrhea syndrome coronavirus (SADS-CoV), and porcine deltacoronavirus (PDCoV) have emerged and been prevalent in pig populations in China for the last several years. However, current traditional inactivated and attenuated PEDV vaccines are of limited efficacy against circulating PEDV variants, and there are no commercial vaccines for prevention of PDCoV and SADS-CoV. RNA interference (RNAi) is a powerful tool in therapeutic applications to inhibit viral replication in vitro. In this study, we developed a small interfering RNA generation system that expressed two different short hairpin RNAs (shRNAs) targeting the M gene of PEDV and SADS-CoV and the N gene of PDCoV, respectively. Our results demonstrated that simultaneous expression of these specific shRNA molecules inhibited expression of PEDV M gene, SADS-CoV M gene, and PDCoV N gene RNA by 99.7%, 99.4%, and 98.8%, respectively, in infected cell cultures. In addition, shRNA molecules significantly restricted the expression of M and N protein, and impaired the replication of PEDV, SADS-CoV, and PDCoV simultaneously. Taken together, this shRNAs expression system not only is proved to be a novel approach for studying functions of various genes synchronously, but also developed to test aspects of a potential therapeutic option for treatment and prevention of multiple SECoV infections.
has issue date	2019-06-30(xsd:dateTime)
bibo:doi	10.1016/j.antiviral.2019.03.010
bibo:pmid	30905822
has license	els-covid
sha1sum (hex)	a73149de69132032bc769a2d43c6a98cf663a069
schema:url	https://doi.org/10.1016/j.antiviral.2019.03.010
resource representing a document's title	Significant inhibition of re-emerged and emerging swine enteric coronavirus in vitro using the multiple shRNA expression vector
has PubMed Central identifier	PMC7113732
has PubMed identifier	30905822
schema:publication	Antiviral Research
resource representing a document's body	covid:a73149de69132032bc769a2d43c6a98cf663a069#body_text
is schema:about of	named entity 'targeting' named entity 'expression system' named entity 'China' named entity 'gene' named entity 'hairpin' named entity 'expression' named entity 'porcine' named entity 'swine' named entity 'short' named entity 'enteric' named entity 'SWINE ENTERIC CORONAVIRUS' named entity 'INHIBITED' named entity 'PORCINE DELTACORONAVIRUS' named entity 'LIMITED' named entity 'POTENTIAL' named entity 'EXPRESSION SYSTEM' named entity 'APPLICATIONS' named entity 'TOOL' named entity 'GENERATION' named entity 'MOLECULES' named entity 'VACCINES' named entity 'CORONAVIRUSES' named entity 'REPLICATION' named entity 'RNAS' named entity 'SYSTEM' named entity 'MULTIPLE' named entity 'SHORT' named entity 'GENES' named entity 'RNA' named entity 'APPROACH' named entity 'CHINA' named entity 'HAVE' covid:arg/a73149de69132032bc769a2d43c6a98cf663a069 named entity 'EXPRESSION VECTOR' named entity 'EXPRESSION' named entity 'IN VITRO' named entity 'VARIOUS' named entity 'BUT' named entity 'EXPRESSED' named entity 'SIGNIFICANT' named entity 'PREVALENT' named entity 'DIFFERENT' named entity 'FUNCTIONS' named entity 'ATTENUATED' named entity 'POWERFUL' named entity 'CELL CULTURES' named entity 'N GENE' named entity 'M GENE' named entity 'INCLUDING' named entity 'RNA INTERFERENCE' named entity 'INFECTIONS' named entity 'CURRENT' named entity 'THERAPEUTIC' named entity 'IMPAIRED' named entity 'NOVEL' named entity 'INFECTED CELL' named entity 'SIMULTANEOUS' named entity 'TREATMENT' named entity 'ENTERIC' named entity 'CIRCULATING' named entity 'ADDITION' named entity 'POPULATIONS' named entity 'SWINE ACUTE DIARRHEA SYNDROME CORONAVIRUS' named entity 'THESE' named entity 'TO TEST' named entity 'IS A' named entity 'INHIBITION' named entity 'RESTRICTED' named entity 'TRADITIONAL' named entity 'N PROTEIN'

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