About: Improving an Escherichia coli-based biocatalyst for terpenol glycosylation by variation of the expression system

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About: Improving an Escherichia coli-based biocatalyst for terpenol glycosylation by variation of the expression system Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Improving an Escherichia coli-based biocatalyst for terpenol glycosylation by variation of the expression system
Creator	Rüdiger, Julian Schwab, Wilfried
Source	Medline; PMC
abstract	Glycosides are becoming increasingly more relevant for various industries as low-cost whole-cell-biocatalysts are now available for the manufacture of glycosides. However, there is still a need to optimize the biocatalysts. The aim of this work was to increase the titre of terpenyl glucosides in biotransformation assays with E. coli expressing VvGT14ao, a glycosyltransferase gene from grape (Vitis vinifera). Seven expression plasmids differing in the resistance gene, origin of replication, promoter sequence, and fusion protein tag were generated and transformed into four different E. coli expression strains, resulting in 18 strains that were tested for glycosylation efficiency with terpenols and a phenol. E. coli BL21(DE3)/pET-SUMO_VvGT14ao yielded the highest titres. The product concentration was improved 8.6-fold compared with E. coli BL21(DE3)pLysS/pET29a_VvGT14ao. The selection of a small solubility-enhancing protein tag and exploitation of the T7 polymerase-induction system allowed the formation of increased levels of functional recombinant protein, thereby improving the performance of the whole-cell biocatalyst. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s10295-019-02184-4) contains supplementary material, which is available to authorized users.
has issue date	2019-05-06(xsd:dateTime)
bibo:doi	10.1007/s10295-019-02184-4
bibo:pmid	31062116
has license	no-cc
sha1sum (hex)	a76edbe4491a7212dbb9a2083a8870cd3057fb66
schema:url	https://doi.org/10.1007/s10295-019-02184-4
resource representing a document's title	Improving an Escherichia coli-based biocatalyst for terpenol glycosylation by variation of the expression system
has PubMed Central identifier	PMC7088306
has PubMed identifier	31062116
schema:publication	J Ind Microbiol Biotechnol
resource representing a document's body	covid:a76edbe4491a7212dbb9a2083a8870cd3057fb66#body_text
is schema:about of	named entity '2C CELL' named entity 'The selection' named entity 'BIOCATALYST' named entity 'ORIGINAL' named entity 'INDUSTRIES' named entity 'TO INCREASE' named entity 'RELEVANT' named entity 'STRAINS' named entity 'MANUFACTURE' named entity 'PET' named entity 'WORK' named entity 'TRANSFORMED' named entity 'BIOCATALYST' named entity 'BL21' named entity 'TESTED FOR' named entity 'GLUCOSIDES' named entity '8.6' named entity 'EXPRESSION' named entity 'AVAILABLE' named entity 'DIFFERENT' named entity 'VARIATION' named entity 'PAPER' named entity 'LOW' named entity 'AIM' named entity 'VARIOUS' named entity 'PRODUCT' named entity 'EXPRESSION SYSTEM' named entity 'IMPROVING' named entity 'CELL CULTURE' named entity 'ESCHERICHIA COLI' named entity 'BASED' named entity 'FERMENTATION' named entity 'TERPENOL' named entity 'POLYMERASE' named entity 'SEVEN' named entity 'COMPARED' named entity 'RECOMBINANT PROTEIN' named entity 'GLYCOSYLTRANSFERASE GENE' named entity 'GENERATED' named entity 'E. COLI' named entity 'IMPROVING' named entity 'FORMATION' named entity 'SYSTEM' named entity 'PLASMIDS' named entity 'BIOTRANSFORMATION' named entity 'SELECTION' named entity 'EFFICIENCY' named entity 'PERFORMANCE' named entity 'PROMOTER SEQUENCE' named entity 'ENHANCING' named entity 'BIOCATALYSTS' named entity 'GLYCOSYLATION' named entity 'BIOENGINEERING' named entity 'GRAPE' named entity 'PROTEIN TAG' named entity 'GLYCOSIDES' named entity 'HIGHEST' named entity 'FUSION PROTEIN' named entity 'ALLOWED' named entity 'COST' named entity 'INDUCTION' named entity 'RESULTING IN' named entity 'TERPENOLS' named entity 'A PHENOL' named entity 'IMPROVED' named entity 'RESISTANCE GENE' named entity 'FOLD' named entity 'GLYCOSYLATION' named entity 'SMALL' named entity 'SUMO' named entity 'CONCENTRATION' named entity 'INCREASED' named entity 'LEVELS' named entity 'NEED' named entity 'TITRE' named entity 'SOLUBILITY' named entity 'ORIGIN OF REPLICATION' named entity 'OPTIMIZE' named entity 'CELL' named entity 'FUNCTIONAL'

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