About: Deep mutational scanning of SARS-CoV-2 receptor binding domain reveals constraints on folding and ACE2 binding

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About: Deep mutational scanning of SARS-CoV-2 receptor binding domain reveals constraints on folding and ACE2 binding Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Deep mutational scanning of SARS-CoV-2 receptor binding domain reveals constraints on folding and ACE2 binding
Creator	Veesler, David Bowen, John Walls, Alexandra Bloom, Jesse Hilton, Sarah Tortorici, M Crawford, Katharine Greaney, Allison Navarro, Mary Starr, Tyler
source	BioRxiv; Medline; PMC
abstract	The receptor binding domain (RBD) of the SARS-CoV-2 spike glycoprotein mediates viral attachment to ACE2 receptor, and is a major determinant of host range and a dominant target of neutralizing antibodies. Here we experimentally measure how all amino-acid mutations to the RBD affect expression of folded protein and its affinity for ACE2. Most mutations are deleterious for RBD expression and ACE2 binding, and we identify constrained regions on the RBD’s surface that may be desirable targets for vaccines and antibody-based therapeutics. But a substantial number of mutations are well tolerated or even enhance ACE2 binding, including at ACE2 interface residues that vary across SARS-related coronaviruses. However, we find no evidence that these ACE2-affinity enhancing mutations have been selected in current SARS-CoV-2 pandemic isolates. We present an interactive visualization and open analysis pipeline to facilitate use of our dataset for vaccine design and functional annotation of mutations observed during viral surveillance.
has issue date	2020-06-17(xsd:dateTime)
bibo:doi	10.1101/2020.06.17.157982
bibo:pmid	32587970
has license	cc-by
sha1sum (hex)	a7b90dc65be5c70ae19a5eccdecb284d0f310bfa
schema:url	https://doi.org/10.1101/2020.06.17.157982
resource representing a document's title	Deep mutational scanning of SARS-CoV-2 receptor binding domain reveals constraints on folding and ACE2 binding
has PubMed Central identifier	PMC7310626
has PubMed identifier	32587970
schema:publication	bioRxiv
resource representing a document's body	covid:a7b90dc65be5c70ae19a5eccdecb284d0f310bfa#body_text
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