About: Evaluating the efficacy of RT-qPCR SARS-CoV-2 direct approaches in comparison to RNA extraction

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About: Evaluating the efficacy of RT-qPCR SARS-CoV-2 direct approaches in comparison to RNA extraction Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Evaluating the efficacy of RT-qPCR SARS-CoV-2 direct approaches in comparison to RNA extraction
Creator	Beth-Din, Adi Israeli, Ofir Laskar, Orly Paran, Nir Stein, Dana Yitzhaki, Shmuel Atiya-Nasagi, Yafit Weiss, Shay Lazar, Shirley Milrot, Elad Schuster, Ofir
Source	BioRxiv
abstract	SARS-CoV-2 genetic identification is based on viral RNA extraction prior to RT-qPCR assay, however recent studies support the elimination of the extraction step. Herein, we assessed the RNA extraction necessity, by comparing RT-qPCR efficacy in several direct approaches vs. the gold standard RNA extraction, in detection of SARS-CoV-2 from laboratory samples as well as clinical Oro-nasopharyngeal SARS-CoV-2 swabs. Our findings show advantage for the extraction procedure, however a direct no-buffer approach might be an alternative, since it identified up to 70% of positive clinical specimens.
has issue date	2020-06-10(xsd:dateTime)
bibo:doi	10.1101/2020.06.10.144196
has license	biorxiv
sha1sum (hex)	a987e584291a44e8bbab14f9909629b8bba57853
schema:url	https://doi.org/10.1101/2020.06.10.144196
resource representing a document's title	Evaluating the efficacy of RT-qPCR SARS-CoV-2 direct approaches in comparison to RNA extraction
schema:publication	bioRxiv
resource representing a document's body	covid:a987e584291a44e8bbab14f9909629b8bba57853#body_text
is schema:about of	named entity 'viral' named entity 'laboratory' named entity 'samples' named entity 'assessed' named entity 'BASED' named entity 'SARS-COV-2' named entity 'EXTRACTION' named entity 'ORO-' named entity 'PRIOR TO' named entity 'MIGHT BE' named entity 'UP TO' named entity 'swabs' named entity 'SARS-CoV-2' named entity 'RNA extraction' named entity 'SARS-CoV-2' named entity 'RT-qPCR' named entity 'clinical samples' named entity 'digital PCR' named entity 'morbidity' named entity 'RNA extraction' named entity 'clinical diagnosis' named entity 'Reagent' named entity 'SARS-CoV-2' named entity 'nucleic acids' named entity 'Lysis' named entity 'Munich' named entity 'RNA extraction' named entity 'COVID-19 pandemic' named entity 'virions' named entity 'reverse transcriptase quantitative PCR' named entity 'RNA extraction' named entity 'Bundeswehr' named entity 'clinical samples' named entity 'RT-qPCR' named entity 'RNA' named entity 'Viral' named entity 'standard deviation' named entity 'SARS-CoV-2' named entity 'plaque forming units' named entity 'RNA extraction' named entity 'SARS-CoV-2' named entity 'diagnostic procedure' named entity 'RNA extraction' named entity 'Viral' named entity 'Berlin' named entity 'Microbiology' named entity 'SARS-CoV-2' named entity 'GISAID' named entity 'clinical samples' named entity 'standard deviation' named entity 'RNA extraction' named entity 'clinical samples' named entity 'Vero E6' named entity 'Germany' named entity 'nasopharyngeal swab' named entity 'clinical samples' named entity 'RNA isolation' named entity 'RT-qPCR' named entity 'RNA' named entity 'RT-qPCR' named entity 'Biomek' named entity 'Virus' named entity 'randomly selected' named entity 'gene' named entity 'titer' named entity 'RT-qPCR' named entity 'transport medium' named entity 'COVID-19' named entity 'supply chains' named entity 'RNA extraction' named entity 'lysis' named entity 'reagent' named entity 'RNA extraction' named entity 'SARS-CoV-2'

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