About: Matrix stiffness modulates infection of endothelial cells by Listeria monocytogenes via expression of cell surface vimentin

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About: Matrix stiffness modulates infection of endothelial cells by Listeria monocytogenes via expression of cell surface vimentin Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Matrix stiffness modulates infection of endothelial cells by Listeria monocytogenes via expression of cell surface vimentin
Creator	Gardel, Margaret
Source	PMC
abstract	Extracellular matrix stiffness (ECM) is one of the many mechanical forces acting on mammalian adherent cells and an important determinant of cellular function. While the effect of ECM stiffness on many aspects of cellular behavior has been studied previously, how ECM stiffness might mediate susceptibility of host cells to infection by bacterial pathogens is hitherto unexplored. To address this open question, we manufactured hydrogels of varying physiologically relevant stiffness and seeded human microvascular endothelial cells (HMEC-1) on them. We then infected HMEC-1 with the bacterial pathogen Listeria monocytogenes (Lm) and found that adhesion of Lm to host cells increases monotonically with increasing matrix stiffness, an effect that requires the activity of focal adhesion kinase (FAK). We identified cell surface vimentin as a candidate surface receptor mediating stiffness-dependent adhesion of Lm to HMEC-1 and found that bacterial infection of these host cells is decreased when the amount of surface vimentin is reduced. Our results provide the first evidence that ECM stiffness can mediate the susceptibility of mammalian host cells to infection by a bacterial pathogen.
has issue date	2018-07-01(xsd:dateTime)
bibo:doi	10.1091/mbc.e18-04-0228
bibo:pmid	29718765
has license	cc-by-nc-sa
sha1sum (hex)	aad248c894ac1b35e2adad9e23c9cf40dc3c20aa
schema:url	https://doi.org/10.1091/mbc.e18-04-0228
resource representing a document's title	Matrix stiffness modulates infection of endothelial cells by Listeria monocytogenes via expression of cell surface vimentin
has PubMed Central identifier	PMC6080647
has PubMed identifier	29718765
schema:publication	Mol Biol Cell
resource representing a document's body	covid:aad248c894ac1b35e2adad9e23c9cf40dc3c20aa#body_text
is schema:about of	named entity 'MATRIX' named entity 'MEDIATE' named entity 'HOW' named entity 'SUSCEPTIBILITY' named entity 'MAMMALIAN' named entity 'FORCES' named entity 'CELLULAR FUNCTION' named entity 'cell' named entity 'VIMENTIN' named entity 'CELL SURFACE' named entity 'ONE OF' named entity 'PREVIOUSLY' named entity 'STIFFNESS' named entity 'INFECTION' named entity 'EXPRESSION' named entity 'ADHERENT' named entity 'EFFECT' named entity 'MECHANICAL' named entity 'INFECTION' named entity 'LISTERIA MONOCYTOGENES' named entity 'ENDOTHELIAL CELLS' named entity 'HOST CELLS' named entity 'IMPORTANT' named entity 'BACTERIAL PATHOGENS' named entity 'STIFFNESS' named entity 'ECM' named entity 'DETERMINANT' named entity 'BEHAVIOR' named entity 'EXTRACELLULAR MATRIX' named entity 'CELLULAR' named entity 'CELLS' named entity 'ECM' named entity 'acting' named entity 'forces' named entity 'host' named entity 'behavior' named entity 'cellular' named entity 'mechanical' named entity 'adherent cells' named entity 'bacterial pathogens' named entity 'determinant' named entity 'endothelial cells' named entity 'infection' named entity 'Integrin' named entity 'phosphatase' named entity 'protein' named entity 'Eppendorf tube' named entity 'ammonium bicarbonate' named entity 'Western blot' named entity 'shear stresses' named entity 'Wilcoxon rank-sum test' named entity 'NP-40' named entity 'S1C' named entity 'Angiotensin II' named entity 'wild-type' named entity 'acetonitrile' named entity 'vimentin' named entity 'infection' named entity 'GAPDH' named entity 'hydrogels' named entity 'vimentin' named entity 'triplets' named entity 'cell surface' named entity 'FAK' named entity 'bacterial adhesion' named entity 'siRNA' named entity 'InlA' named entity 'cell types' named entity 'basement membrane' named entity 'S1C' named entity 'bacterial adhesion' named entity 'siRNAs' named entity 'receptor' named entity 'FAK' named entity 'cell surface receptors' named entity 'glyceraldehyde 3-phosphate dehydrogenase' named entity 'mammalian cells' named entity 'cell-surface' named entity 'TEMED' named entity 'gene expression' named entity 'Young's modulus'

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