About: Immunocytochemical Localization of the Menkes Copper Transport Protein (ATP7A) to the Trans-Golgi Network

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Immunocytochemical Localization of the Menkes Copper Transport Protein (ATP7A) to the Trans-Golgi Network Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Immunocytochemical Localization of the Menkes Copper Transport Protein (ATP7A) to the Trans-Golgi Network
Creator	Adam, Ayla Dierick, Herman Escara-Wilke, June Glover, Thomas
Source	PMC
abstract	We have generated polyclonal antibodies against the amino-terminal third of the Menkes protein (ATP7A; MNK) by immunizing rabbits with a histidine-tagged MNK fusion construct containing metal-binding domains 1–4. The purified antibodies were used in Western analysis of cell lysates and in indirect immunofluorescence experiments on cultured cells. On Western blots, the antibodies recognized the ∼165 kDa MNK protein in CHO cells and human fibroblasts. No MNK signal could be detected in fibroblasts from a patient with Menkes disease or in Hep3B hepatocellular carcinoma cells, confirming the specificity of the antibodies. Immunocytochemical analysis of CHO cells and human fibroblasts showed a distinct perinuclear signal corresponding to the pattern of the Golgi complex. This staining pattern was similar to that of α-mannosidase II which is a known resident enzyme of the Golgi complex. Using brefeldin A, a fungal inhibitor of protein secretion, we further demonstrated that the MNK protein is localized to the trans-Golgi network. This data provides direct evidence for a subcellular localization of the MNK protein which is similar to the proposed vacuolar localization of Ccc2p, the yeast homolog of MNK and WND (ATP7B), the Wilson disease gene product. In light of the proposed role of MNK both in subcellular copper trafficking and in copper efflux, these data suggest a model for how these two processes are linked and represent an important step in the functional analysis of the MNK protein.
has issue date	1997-03-01(xsd:dateTime)
bibo:doi	10.1093/hmg/6.3.409
bibo:pmid	9147644
has license	no-cc
sha1sum (hex)	abbd7c04aaaedd34c2210c11153145c9e2bf46bc
schema:url	https://doi.org/10.1093/hmg/6.3.409
resource representing a document's title	Immunocytochemical Localization of the Menkes Copper Transport Protein (ATP7A) to the Trans-Golgi Network
has PubMed Central identifier	PMC7185191
has PubMed identifier	9147644
schema:publication	Hum Mol Genet
resource representing a document's body	covid:abbd7c04aaaedd34c2210c11153145c9e2bf46bc#body_text
is schema:about of	named entity 'MNK' named entity 'Western' named entity 'indirect immunofluorescence' named entity 'protein' named entity 'CHO cells' named entity 'represent' named entity 'confirming' named entity 'antibodies' named entity 'Wilson' named entity 'pattern' named entity 'trans-Golgi network' named entity 'CHO CELLS' named entity 'CONFIRMING' named entity 'KDA' named entity 'TRAFFICKING' named entity 'WESTERN BLOTS' named entity 'EFFLUX' named entity 'SUGGEST' named entity 'EXPERIMENTS' named entity 'INHIBITOR' named entity 'ENZYME' named entity 'LOCALIZED' named entity 'MENKES PROTEIN' named entity 'STEP' named entity 'USED' named entity 'MNK' named entity 'protein' named entity 'data' named entity 'generated' named entity 'staining' named entity 'WND' named entity 'experiments' named entity 'localization' named entity 'trafficking' named entity 'pattern' named entity 'fungal' named entity 'This' named entity 'trans-Golgi network' named entity 'ATP7A' named entity 'MNK' named entity 'gene' named entity 'fibroblasts' named entity 'staining' named entity 'Menkes disease' named entity 'MNK' named entity 'Immunocytochemical' named entity 'MNK' named entity 'trans-Golgi network' named entity 'binding domains' named entity 'Golgi' named entity 'MNK' named entity 'protein' named entity 'MNK' named entity 'fusion construct' named entity 'indirect immunofluorescence' named entity 'amino-terminal' named entity 'copper' named entity 'transport protein' named entity 'room temperature' named entity 'copper' named entity 'adsorbed' named entity 'TGN' named entity 'PBS' named entity 'yeast' named entity 'MNK' named entity 'cDNA' named entity 'cultured cells' named entity 'streptomycin' named entity 'Menkes disease' named entity 'cDNA' named entity 'intracellular transport' named entity 'MNK' named entity 'Freund's complete adjuvant' named entity 'subcellular compartment' named entity 'pH 8' named entity 'PBS' named entity 'conjugated' named entity 'liver'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software