About: Middle East respiratory syndrome coronavirus in dromedary camels: an outbreak investigation

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About: Middle East respiratory syndrome coronavirus in dromedary camels: an outbreak investigation Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Middle East respiratory syndrome coronavirus in dromedary camels: an outbreak investigation
Creator	Koopmans, Marion Al Khal, Abdullatif Al-Thani, Mohamed Alhajri, Farhoud Bermingham, Alison Diab, Ayman Farag, Elmoubasher Galiano, Monica Ghobashy, Hazem Godeke, Gert-Jan Jonges, Marcel Myers, Richard Al-Marri, Salih Alhajri, Mohd Haagmans, Bart Raj, Stalin Al Romaihi, Hamad Dhahiry, Al Osterhaus, E Reusken, E
source	Elsevier; Medline; PMC
abstract	BACKGROUND: Middle East respiratory syndrome coronavirus (MERS-CoV) causes severe lower respiratory tract infection in people. Previous studies suggested dromedary camels were a reservoir for this virus. We tested for the presence of MERS-CoV in dromedary camels from a farm in Qatar linked to two human cases of the infection in October, 2013. METHODS: We took nose swabs, rectal swabs, and blood samples from all camels on the Qatari farm. We tested swabs with RT-PCR, with amplification targeting the E gene (upE), nucleocapsid (N) gene, and open reading frame (ORF) 1a. PCR positive samples were tested by different MERS-CoV specific PCRs and obtained sequences were used for phylogentic analysis together with sequences from the linked human cases and other human cases. We tested serum samples from the camels for IgG immunofluorescence assay, protein microarray, and virus neutralisation assay. FINDINGS: We obtained samples from 14 camels on Oct 17, 2013. We detected MERS-CoV in nose swabs from three camels by three independent RT-PCRs and sequencing. The nucleotide sequence of an ORF1a fragment (940 nucleotides) and a 4·2 kb concatenated fragment were very similar to the MERS-CoV from two human cases on the same farm and a MERS-CoV isolate from Hafr-Al-Batin. Eight additional camel nose swabs were positive on one or more RT-PCRs, but could not be confirmed by sequencing. All camels had MERS-CoV spike-binding antibodies that correlated well with the presence of neutralising antibodies to MERS-CoV. INTERPRETATION: Our study provides virological confirmation of MERS-CoV in camels and suggests a recent outbreak affecting both human beings and camels. We cannot conclude whether the people on the farm were infected by the camels or vice versa, or if a third source was responsible. FUNDING: European Union projects EMPERIE (contract number 223498), ANTIGONE (contract number 278976), and the VIRGO consortium.
has issue date	2013-12-17(xsd:dateTime)
bibo:doi	10.1016/s1473-3099(13)70690-x
bibo:pmid	24355866
has license	no-cc
sha1sum (hex)	abe97b9d4046417edffcac7a7488ae012da5265a
schema:url	https://doi.org/10.1016/s1473-3099%2813%2970690-x
resource representing a document's title	Middle East respiratory syndrome coronavirus in dromedary camels: an outbreak investigation
has PubMed Central identifier	PMC7106553
has PubMed identifier	24355866
schema:publication	Lancet Infect Dis
resource representing a document's body	covid:abe97b9d4046417edffcac7a7488ae012da5265a#body_text
is schema:about of	named entity 'virus' named entity 'MERS-CoV' named entity 'Middle East respiratory syndrome' covid:arg/abe97b9d4046417edffcac7a7488ae012da5265a named entity 'Background' named entity 'coronavirus' named entity 'suggested' named entity 'dromedary camels' named entity 'SARS-CoV' named entity 'TaqMan' named entity 'HKU5' named entity 'virus' named entity 'serum' named entity 'serum neutralisation' named entity 'Arabian peninsula' named entity 'MERS-CoV' named entity 'Qatar' named entity 'MERS-CoV' named entity 'MERS-CoV' named entity 'Qatar' named entity 'human beings' named entity 'animal reservoirs' named entity 'RT-PCR' named entity 'incubation' named entity 'RT-PCR' named entity 'Huh-7' named entity 'pneumonitis' named entity 'MERS-CoV' named entity 'substitution model' named entity 'MERS-CoV' named entity 'dromedary camels' named entity 'reverse transcribed' named entity 'fetal bovine serum' named entity '37°C' named entity 'infection' named entity 'acute respiratory distress syndrome' named entity 'RNA' named entity 'Erasmus MC' named entity 'Huh-7' named entity 'gene' named entity 'Applied Biosystems' named entity 'infection' named entity 'RT-PCR' named entity 'gene' named entity 'virus' named entity 'MERS-CoV' named entity 'Qiagen' named entity 'MERS-CoV' named entity 'MERS-CoV' named entity 'phylogenetic trees' named entity 'nucleotide' named entity 'RNA' named entity 'TaqMan' named entity 'MERS-CoV' named entity 'sputum' named entity 'Virus' named entity 'dry ice' named entity 'coronavirus' named entity 'Rotterdam' named entity 'RNA' named entity 'dromedary camels' named entity 'cell lines'

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