About: Detection of bovine coronavirus in feces by reversed passive hemagglutination

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Detection of bovine coronavirus in feces by reversed passive hemagglutination
Creator	Asagi, M Matumoto, M Inaba I, Y Ka~eko I, N Mivi~a I, Y Sago, K Tokuiiisa I, S
Source	Medline; PMC
abstract	A reversed passive hemagglutination (RPHA) method was developed for the detection of bovine coronavirus in fecal specimens. Sheep erythrocytes fixed with glutaraldehyde, and then treated with tannic acid were coated with anti-bovine coronavirus rabbit antibodies purified by affinity chromatography using bovine coronavirus linked to Sepharose 4B. The RPHA test was carried out by a microtiter method. Erythrocytes coated with purified specific antibodies were agglutinated by bovine coronavirus, but not by bovine rotavirus or enterovirus. The reaction was inhibited by antiserum to bovine coronavirus, confirming the specificity of the reaction. The RPHA test detected bovine coronavirus in 13 of 22 fecal specimens (59 per cent), from natural cases of diarrhea, while the positive rates were only 14 per cent (3/22) and 22 per cent (5/22) for immunofluorescent staining of primary cultures of calf kidney cells infected with the specimens, and immune electron microscopy respectively. The advantages of the RPHA method are its simplicity, high sensitivity and rapidity.
has issue date	1984-01-01(xsd:dateTime)
bibo:doi	10.1007/bf01315291
bibo:pmid	6367710
has license	no-cc
sha1sum (hex)	ac275323b2569c13707cd9f5768360d6cbc37b69
schema:url	https://doi.org/10.1007/bf01315291
resource representing a document's title	Detection of bovine coronavirus in feces by reversed passive hemagglutination
has PubMed Central identifier	PMC7086679
has PubMed identifier	6367710
schema:publication	Arch Virol
resource representing a document's body	covid:ac275323b2569c13707cd9f5768360d6cbc37b69#body_text
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