About: Simultaneous detection of respiratory viruses in children with acute respiratory infection using two different multiplex reverse transcription-PCR assays

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About: Simultaneous detection of respiratory viruses in children with acute respiratory infection using two different multiplex reverse transcription-PCR assays Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Simultaneous detection of respiratory viruses in children with acute respiratory infection using two different multiplex reverse transcription-PCR assays
Creator	Wang, Wei Vabret, Astrid Zhang, Jing Buchy, Philippe Deubel, Vincent Mardy, Sek Hou, Lili Ren, Peijun Sheng, Jun Yan, Huajie Freymuth, Francois
Source	Elsevier; Medline; PMC
abstract	A 4-tube multiplex RT-PCR (mRT-PCR), which showed higher sensitivity over conventional methods, was previously developed for the diagnosis of 14 viral pathogens of the respiratory tract. Herein the mRT-PCR was compared to the commercial Luminex mPCR-microsphere flow cytometry assay (Resplex II) which allows the detection of 12 different viruses. Eleven different viruses were identified in 91 nasopharyngeal swabs of children with acute respiratory infection, influenza A (IAV) and B, respiratory syncytial virus (RSV), human rhinovirus (hRhV), human echovirus, parainfluenza viruses (PIV) 1, 2, 3 and 4, human metapneumovirus (hMPV), and human coronavirus NL63. The results of the two techniques showed 53 and 40 positive patients by the Resplex II assay and mRT-PCR, respectively, with a concordance in 35 positive and 33 negative patients (74.7%). Individual RT-PCR tests were performed to control viruses not simultaneously detected by the two multiplex assays. The major virus misdiagnosed by mRT-PCR was IAV whereas the major viruses misdiagnosed by Resplex II were PIV1, 3 and 4. The mRT-PCR remains a simple, rapid, and specific assay for the specific detection of respiratory viruses, and can be easily implemented with standards in clinical laboratories at a low cost.
has issue date	2009-07-26(xsd:dateTime)
bibo:doi	10.1016/j.jviromet.2009.07.004
bibo:pmid	19638288
has license	no-cc
sha1sum (hex)	ac44b168cb2445657d3d041c70cd70ad6f540d5b
schema:url	https://doi.org/10.1016/j.jviromet.2009.07.004
resource representing a document's title	Simultaneous detection of respiratory viruses in children with acute respiratory infection using two different multiplex reverse transcription-PCR assays
has PubMed Central identifier	PMC7119720
has PubMed identifier	19638288
schema:publication	J Virol Methods
resource representing a document's body	covid:ac44b168cb2445657d3d041c70cd70ad6f540d5b#body_text
is schema:about of	named entity 'REVERSE TRANSCRIPTION' named entity 'parainfluenza' named entity 'rapid' named entity 'nasopharyngeal' named entity 'assay' named entity 'reverse' named entity 'viruses' named entity 'DETECTION' named entity 'RESPIRATORY TRACT' named entity 'SIMULTANEOUSLY' named entity 'EASILY' named entity 'INFLUENZA A' named entity 'DIAGNOSIS' named entity 'RESPIRATORY VIRUSES' named entity 'ALLOWS' named entity 'RESULTS' named entity 'CHILDREN' named entity 'VIRAL PATHOGENS' named entity 'SPECIFIC' named entity 'RESPIRATORY SYNCYTIAL VIRUS' named entity 'ACUTE RESPIRATORY INFECTION' named entity 'MULTIPLEX' named entity 'SENSITIVITY' named entity 'LOW' named entity 'HUMAN METAPNEUMOVIRUS' named entity 'COST' named entity 'RESPIRATORY VIRUSES' named entity 'USING' named entity 'RT-PCR' named entity 'COMMERCIAL' named entity 'SIMPLE' named entity 'TESTS' named entity 'METHODS' named entity 'MULTIPLEX' named entity 'HUMAN ECHOVIRUS' named entity 'HUMAN CORONAVIRUS NL63' named entity 'HUMAN RHINOVIRUS' named entity 'RAPID' named entity 'LUMINEX' named entity 'VIRUSES' named entity 'MRT' named entity 'FLOW CYTOMETRY ASSAY' named entity 'PERFORMED' named entity 'CHILDREN' named entity 'MICROSPHERE' named entity 'INDIVIDUAL' named entity 'DETECTED' named entity 'CLINICAL LABORATORIES' named entity 'PARAINFLUENZA' named entity 'SIMULTANEOUS' named entity 'CONVENTIONAL' named entity 'CONCORDANCE' named entity 'TUBE' named entity 'NEGATIVE' named entity 'VIRUS' named entity 'COMPARED' named entity 'STANDARDS' named entity 'MAJOR' named entity 'PREVIOUSLY' named entity 'PCR' named entity 'TECHNIQUES' named entity 'ASSAY' named entity 'HIGHER' named entity 'SWABS' named entity 'PATIENTS' named entity 'NASOPHARYNGEAL' named entity 'PCR' named entity 'DIFFERENT' named entity 'IDENTIFIED' named entity 'DETECTION' named entity 'DIFFERENT'

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