About: Rapid, reliable, and cheap point-of-care bulk testing for SARS-CoV-2 by combining hybridization capture with improved colorimetric LAMP (Cap-iLAMP)

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About: Rapid, reliable, and cheap point-of-care bulk testing for SARS-CoV-2 by combining hybridization capture with improved colorimetric LAMP (Cap-iLAMP) Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Rapid, reliable, and cheap point-of-care bulk testing for SARS-CoV-2 by combining hybridization capture with improved colorimetric LAMP (Cap-iLAMP)
Creator	Pääbo, Svante Borte, Stephan Nickel, Olaf Bokelmann, Lukas Maricic, Tomislav Meyer, Matthias Riesenberg, Stephan
source	MedRxiv
abstract	Efforts to contain the spread of SARS-CoV-2 have spurred the need for reliable, rapid, and cost-effective diagnostic methods which can easily be applied to large numbers of people. However, current standard protocols for the detection of viral nucleic acids while sensitive, require a high level of automation, sophisticated laboratory equipment and trained personnel to achieve throughputs that allow whole communities to be tested on a regular basis. Here we present Cap-iLAMP (capture and improved loop-mediated isothermal amplification). This method combines a hybridization capture-based RNA extraction of non-invasive gargle lavage samples to concentrate samples and remove inhibitors with an improved colorimetric RT-LAMP assay and smartphone-based color scoring. Cap-iLAMP is compatible with point-of-care testing and enables the detection of SARS-CoV-2 positive samples in less than one hour. In contrast to direct addition of the sample to improved LAMP (iLAMP), Cap-iLAMP does not result in false positives and single infected samples can be detected in a pool among 25 uninfected samples, thus reducing the technical cost per test to ~1 Euro per individual.
has issue date	2020-08-06(xsd:dateTime)
bibo:doi	10.1101/2020.08.04.20168617
has license	medrxiv
sha1sum (hex)	acb99e1fd51b5f3e0c4748f5dddb114951964096
schema:url	https://doi.org/10.1101/2020.08.04.20168617
resource representing a document's title	Rapid, reliable, and cheap point-of-care bulk testing for SARS-CoV-2 by combining hybridization capture with improved colorimetric LAMP (Cap-iLAMP)
resource representing a document's body	covid:acb99e1fd51b5f3e0c4748f5dddb114951964096#body_text
is schema:about of	named entity 'LAMP' named entity 'reaction' named entity 'regular' named entity 'negative' named entity 'samples' named entity 'sensitive' named entity 'individual' named entity 'cheap' named entity 'LOOP-MEDIATED ISOTHERMAL AMPLIFICATION' named entity 'DETECTION' named entity 'HIGH LEVEL' named entity 'CONTRAST' named entity '28A' named entity 'DOES NOT' named entity 'SARS-COV-2' named entity 'COPY' named entity 'CURRENT' named entity 'PROTOCOLS' named entity 'CONCENTRATE' named entity 'POSITIVE' named entity 'DIFFERENT' named entity 'BASIS' named entity 'CV15' named entity 'HUE' named entity '20%' named entity 'CV1' named entity 'LAMP' named entity 'DIAGNOSTIC' named entity 'improved' named entity 'curves' named entity 'automation' named entity 'lead' named entity 'samples' named entity 'positive control' named entity 'copy numbers' named entity 'LAMP' named entity 'cost' named entity 'high level' named entity 'SARS-CoV-2' named entity 'improved' named entity 'samples' named entity 'reducing' named entity 'lavage' named entity 'point-of-care testing' named entity 'detected' named entity 'LAMP' named entity 'Fig' named entity 'Here' named entity 'Amplification' named entity 'loop-mediated isothermal amplification' named entity 'artificial' named entity 'RNA' named entity 'false positives' named entity 'SARS-CoV-2' named entity 'RNA' named entity 'hybridization' named entity 'primers' named entity 'nucleic acids' named entity 'samples' named entity 'parasites' named entity 'peer review' named entity 'Supp' named entity 'hybridization' named entity 'viral RNA' named entity 'LiCl' named entity 'RNA isolation' named entity 'RT-qPCR' named entity 'paramagnetic' named entity 'RNA extraction' named entity 'coronavirus disease 2019' named entity 'cleavage product' named entity 'gene' named entity 'helicase' named entity 'inorganic pyrophosphatase' named entity 'alkaline' named entity 'gene' named entity 'medRxiv' named entity 'false negative' named entity 'Supp'

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