About: A duplex real-time RT-PCR assay for the detection of California serogroup and Cache Valley viruses

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About: A duplex real-time RT-PCR assay for the detection of California serogroup and Cache Valley viruses Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	A duplex real-time RT-PCR assay for the detection of California serogroup and Cache Valley viruses
Creator	Wang, Heng Kramer, Laura Nattanmai, Seela Bernard, Kristen Tavakoli, Norma
source	Elsevier; Medline; PMC
abstract	A duplex TaqMan real-time RT-PCR assay was developed for the detection of California (CAL) serogroup viruses and Cache Valley virus (CVV), for use in human surveillance. The targets selected for the assay were the sequences encoding the nucleocapsid protein of CAL and the G1 glycoprotein of CVV. Conserved regions were selected by aligning genetic sequences from various strains available in the GenBank database. Primers and probes were selected in conserved regions. The assay sensitivity was 75 gene copies (gc)/reaction for CAL serogroup viruses and 30 gc/reaction for CVV. The performance of the assay was linear over at least 6 log(10) gc. The assay was specific, given that it did not cross-react with a variety of pathogens. It did however, detect 11 viruses within the CAL serogroup, and 12 CVV isolates. The use of an internal control ensured that possible inefficiency in nucleic acid extraction or PCR inhibition would be detected.
has issue date	2009-10-01(xsd:dateTime)
bibo:doi	10.1016/j.diagmicrobio.2009.07.001
bibo:pmid	19748425
has license	green-oa
sha1sum (hex)	ad7ea210a6f7a873a444146e8f8a9c5882a9dee0
schema:url	https://doi.org/10.1016/j.diagmicrobio.2009.07.001
resource representing a document's title	A duplex real-time RT-PCR assay for the detection of California serogroup and Cache Valley viruses
has PubMed Central identifier	PMC2774246
has PubMed identifier	19748425
schema:publication	Diagnostic Microbiology and Infectious Disease
resource representing a document's body	covid:ad7ea210a6f7a873a444146e8f8a9c5882a9dee0#body_text
is schema:about of	named entity 'serogroup' named entity 'nucleocapsid' named entity 'CAL' named entity 'serogroup' named entity 'regions' named entity 'inhibition' named entity 'selected' named entity 'serogroup' named entity 'DETECTED' named entity 'GIVEN' named entity 'REGIONS' named entity 'PROBES' named entity 'SURVEILLANCE' named entity 'SPECIFIC' named entity 'LINEAR' named entity 'REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION' named entity 'INEFFICIENCY' named entity 'DETECTION' named entity 'HUMAN' named entity 'USE' named entity 'VIRUSES' named entity 'ISOLATES' named entity 'CONTROL' named entity 'SEROGROUP' named entity 'PROTEIN ' named entity 'ALIGNING' named entity 'NUCLEIC ACID EXTRACTION' named entity 'SEQUENCES' named entity 'COPIES' named entity 'REACT' named entity 'GENETIC' named entity 'LOG' named entity 'DUPLEX' named entity 'POSSIBLE' named entity 'PCR' named entity 'NUCLEOCAPSID PROTEIN' named entity 'GENBANK' named entity 'DATABASE' named entity 'INHIBITION' named entity 'CAL' named entity 'DETECT' named entity 'USE OF' named entity 'CALIFORNIA' named entity 'ENCODING' named entity 'TARGETS' named entity 'VARIOUS' named entity 'VIRUSES' named entity 'POLYMERASE CHAIN REACTION ASSAY' named entity 'CALIFORNIA SEROGROUP' named entity 'DETECTION' named entity 'REAL-TIME' named entity 'CROSS' named entity 'VARIETY' named entity 'TAQMAN' named entity 'SENSITIVITY' named entity 'PATHOGENS' named entity 'PERFORMANCE' named entity 'AVAILABLE' named entity 'INTERNAL' named entity 'DID' named entity 'GLYCOPROTEIN' named entity 'GENE' named entity 'CACHE VALLEY VIRUS' named entity 'CONSERVED' named entity 'STRAINS' named entity 'REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION' named entity 'SELECTED' named entity 'REAL-TIME' named entity 'DUPLEX' named entity 'CACHE VALLEY' named entity 'PRIMERS' named entity 'ASSAY' covid:arg/ad7ea210a6f7a873a444146e8f8a9c5882a9dee0 named entity 'GenBank' named entity 'assay' named entity 'The performance' named entity 'extraction'

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