About: First international external quality assessment of molecular diagnostics for Mers-CoV

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	First international external quality assessment of molecular diagnostics for Mers-CoV
Creator	Nowotny, Norbert Thiel, Volker Dijkman, Ronald Koopmans, Marion Reusken, Chantal Drosten, Christian Niedrig, Matthias Domingo, Cristina Patel, Pranav Corman, Victor Pas, Suzan
Source	Elsevier; Medline; PMC
abstract	BACKGROUND: Since the discovery of Middle East respiratory syndrome coronavirus (MERS-CoV) in 2012, diagnostic protocols were quickly published and deployed globally. OBJECTIVES: We set out to assess the quality of MERS-CoV molecular diagnostics worldwide. STUDY DESIGN: Both sensitivity and specificity were assessed using 12 samples containing different viral loads of MERS-CoV or common coronaviruses (OC43, 229E, NL63, HKU1). RESULTS: The panel was sent to more than 106 participants, of which 99 laboratories from 6 continents returned 189 panel results.Scores ranged from 100% (84 laboratories) to 33% (1 laboratory). 15% of respondents reported quantitative results, 61% semi-quantitative (Ct-values or time to positivity) and 24% reported qualitative results. The major specific technique used was real-time RT-PCR using the WHO recommended targets upE, ORF1a and ORF1b. The evaluation confirmed that RT-PCRs targeting the ORF1b are less sensitive, and therefore not advised for primary diagnostics. CONCLUSIONS: The first external quality assessment MERS-CoV panel gives a good insight in molecular diagnostic techniques and their performances for sensitive and specific detection of MERS-CoV RNA globally. Overall, all laboratories were capable of detecting MERS-CoV with some differences in sensitivity. The observation that 8% of laboratories reported false MERS-CoV positive single assay results shows room for improvement, and the importance of using confirmatory targets.
has issue date	2015-05-30(xsd:dateTime)
bibo:doi	10.1016/j.jcv.2015.05.022
bibo:pmid	26209385
has license	no-cc
sha1sum (hex)	adfb6209b1ba33b6b1cb11dc9b183f3a9bf074f8
schema:url	https://doi.org/10.1016/j.jcv.2015.05.022
resource representing a document's title	First international external quality assessment of molecular diagnostics for Mers-CoV
has PubMed Central identifier	PMC7106520
has PubMed identifier	26209385
schema:publication	J Clin Virol
resource representing a document's body	covid:adfb6209b1ba33b6b1cb11dc9b183f3a9bf074f8#body_text
is schema:about of	named entity 'improvement' named entity 'quantitative' named entity 'specific' named entity 'The' named entity 'molecular diagnostic' named entity 'MOLECULAR DIAGNOSTICS' named entity 'LABORATORIES' named entity 'CONTINENTS' named entity 'DIFFERENT' named entity 'CONFIRMED' named entity 'ROOM' named entity 'RESULTS' named entity 'CAPABLE' named entity 'DISCOVERY' named entity 'RETURNED' named entity 'OBSERVATION' named entity 'MAJOR' named entity 'PANEL' named entity 'MORE THAN' named entity 'SENSITIVE' named entity 'TIME' named entity 'SPECIFIC' named entity 'QUANTITATIVE' named entity 'TECHNIQUE' named entity 'REPORTED' named entity 'VIRAL' named entity '189' named entity 'MERS-COV RNA' named entity 'SINGLE' named entity 'PERFORMANCES' named entity 'VALUES' named entity 'NL63' named entity 'EVALUATION' named entity 'OVERALL' named entity 'WORLDWIDE' named entity 'SCORES' named entity 'SENSITIVITY' named entity 'SENSITIVITY AND SPECIFICITY' named entity 'USING' named entity 'GLOBALLY' named entity 'RECOMMENDED' named entity 'TARGETS' named entity 'ASSAY' named entity 'QUALITATIVE' named entity 'MIDDLE EAST RESPIRATORY SYNDROME CORONAVIRUS' named entity '2884' named entity 'DEPLOYED' named entity 'INTERNATIONAL' named entity 'UPE' named entity 'DIAGNOSTIC' named entity 'POSITIVE' named entity 'SENT' named entity '229E' named entity 'INSIGHT' named entity 'SEMI-QUANTITATIVE' named entity 'PROTOCOLS' covid:arg/adfb6209b1ba33b6b1cb11dc9b183f3a9bf074f8 named entity 'MERS-COV' named entity 'BACKGROUND' named entity 'TARGETING' named entity 'COMMON' named entity 'EXTERNAL QUALITY ASSESSMENT' named entity 'EXTERNAL QUALITY ASSESSMENT' named entity 'QUALITY' named entity 'DETECTING' named entity 'PARTICIPANTS' named entity 'CONCLUSIONS' named entity 'CONTAINING' named entity 'MOLECULAR DIAGNOSTICS' named entity 'LABORATORY' named entity 'MOLECULAR DIAGNOSTIC TECHNIQUES'

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