About: Inversion Genotyping in the Anopheles gambiae Complex Using High-Throughput Array and Sequencing Platforms

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About: Inversion Genotyping in the Anopheles gambiae Complex Using High-Throughput Array and Sequencing Platforms Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Inversion Genotyping in the Anopheles gambiae Complex Using High-Throughput Array and Sequencing Platforms
Creator	Besansky, Nora Campbell, Nathan Costantini, Carlo Dabire, Roch Della Torre, Alessandra Guelbeogo, Moussa Love, R Pombi, Marco Stephens, Melissa
source	BioRxiv
abstract	Chromosomal inversion polymorphisms have special importance in the Anopheles gambiae complex of malaria vector mosquitoes, due to their role in local adaptation and range expansion. The study of inversions in natural populations is reliant on polytene chromosome analysis by expert cytogeneticists, a process that is limited by the rarity of trained specialists, low throughput, and restrictive sampling requirements. To overcome this barrier, we ascertained tag single nucleotide polymorphisms (SNPs) that are highly correlated with inversion status (inverted or standard orientation). We compared the performance of the tag SNPs using two alternative high throughput molecular genotyping approaches versus traditional cytogenetic karyotyping of the same 960 individual An. gambiae and An. coluzzii mosquitoes sampled from Burkina Faso, West Africa. We show that both molecular approaches yield comparable results, and that either one performs as well or better than cytogenetics in terms of genotyping accuracy. Given the ability of molecular genotyping approaches to be conducted at scale and at relatively low cost without restriction on mosquito sex or developmental stage, molecular genotyping via tag SNPs has the potential to revitalize research into the role of chromosomal inversions in the behavior and ongoing adaptation of An. gambiae and An. coluzzii to environmental heterogeneities.
has issue date	2020-05-28(xsd:dateTime)
bibo:doi	10.1101/2020.05.25.114793
has license	biorxiv
sha1sum (hex)	ae04a46a033e944f82f9e7e3f31dd7d460d9c0f2
schema:url	https://doi.org/10.1101/2020.05.25.114793
resource representing a document's title	Inversion Genotyping in the Anopheles gambiae Complex Using High-Throughput Array and Sequencing Platforms
schema:publication	bioRxiv
resource representing a document's body	covid:ae04a46a033e944f82f9e7e3f31dd7d460d9c0f2#body_text
is schema:about of	named entity 'vector' named entity 'reliant' named entity 'Complex' named entity 'inversions' named entity 'Burkina Faso' named entity 'polymorphisms' named entity 'mosquitoes' named entity 'Burkina Faso' named entity 'An. gambiae' named entity 'homozygotes' named entity 'SNPs' named entity 'polytene' named entity 'statistical power' named entity 'PCR' named entity 'sampling' named entity 'genome' named entity 'genotypes' named entity 'An. gambiae' named entity 'mosquito' named entity 'multilocus' named entity 'paramagnetic' named entity 'homozygote' named entity 'homozygotes' named entity 'genotypic' named entity 'natural variation' named entity 'SNPs' named entity 'genotyping' named entity 'heterogeneities' named entity 'SNPs' named entity 'genotypic' named entity 'experimental designs' named entity 'An. gambiae' named entity 'phenotypes' named entity 'An. gambiae' named entity 'monophyletic' named entity 'genotype' named entity 'genome sequencing' named entity 'SNPs' named entity 'genotype' named entity 'cytogenetics' named entity '95%' named entity 'physiological' named entity 'allele' named entity 'SNPs' named entity 'homozygotes' named entity 'population structure' named entity 'genotyping' named entity 'cytogenetics' named entity 'genotyping' named entity 'taxon' named entity 'primers' named entity 'multilocus' named entity 'SNP' named entity 'mosquito' named entity 'molecular mechanisms' named entity 'genotype' named entity 'mosquito' named entity 'TaqMan' named entity 'nucleotide diversity' named entity 'homozygote' named entity 'molecular methods' named entity 'probability' named entity 'heterozygote' named entity 'An. gambiae' named entity 'Beckman Coulter' named entity 'genotypes' named entity 'tag SNP' named entity 'sampling' named entity 'null hypothesis' named entity 'cytogenetic' named entity 'genotyping' named entity 'An. gambiae' named entity 'mosquito' named entity 'allele' named entity 'Tween 20' named entity '0.9'

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