About: Pathogen transcriptional profile in nasopharyngeal aspirates of children with acute respiratory tract infection

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About: Pathogen transcriptional profile in nasopharyngeal aspirates of children with acute respiratory tract infection Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Pathogen transcriptional profile in nasopharyngeal aspirates of children with acute respiratory tract infection
Creator	Van Ranst, Marc Barral, Aldina Dierckx, Tim Houspie, Lieselot Khouri, Ricardo Wollants, Elke Bouzas, Maiara De Oliveira, Camila Fukutani, Kiyoshi Nascimento-Carvalho, Cristiana Oliveira, Juliana Van Der Gucht, Winke Weyenbergh, Johan
Source	Elsevier; Medline; PMC
abstract	BACKGROUND: Acute respiratory tract infections (ARI) present a significant morbidity and pose a global health burden. Patients are frequently treated with antibiotics although ARI are most commonly caused by virus, strengthening the need for improved diagnostic methods. OBJECTIVES: Detect viral and bacterial RNA in nasopharyngeal aspirates (NPA) from children aged 6–23 months with ARI using nCounter. STUDY DESIGN: A custom-designed nCounter probeset containing viral and bacterial targets was tested in NPA of ARI patients. RESULTS: Initially, spiked control viral RNAs were detectable in ≥6.25 ng input RNA, indicating absence of inhibitors in NPA. nCounter applied to a larger NPA sample (n = 61) enabled the multiplex detection of different pathogens: RNA viruses Parainfluenza virus (PIV 1–3) and RSV A-B in 21%, Human metapneumovirus (hMPV) in 5%, Bocavirus (BoV), CoV, Influenza virus (IV) A in 3% and, Rhinovirus (RV) in 2% of samples, respectively. RSV A-B was confirmed by Real Time PCR (86.2–96.9% agreement). DNA virus (AV) was detected at RNA level, reflecting viral replication, in 10% of samples. Bacterial transcripts from Staphylococcus aureus, Haemophilus influenzae, Streptococcus pneumoniae, Moraxella catarrhalis, Mycoplasma pneumoniae and Chlamydophila pneumoniae were detected in 77, 69, 26, 8, 3 and 2% of samples, respectively. CONCLUSION: nCounter is robust and sensitive for the simultaneous detection of viral (both RNA and DNA) and bacterial transcripts in NPA with low RNA input (<10 ng). This medium-throughput technique will increase our understanding of ARI pathogenesis and may provide an evidence-based approach for the targeted and rational use of antibiotics in pediatric ARI.
has issue date	2015-06-10(xsd:dateTime)
bibo:doi	10.1016/j.jcv.2015.06.005
bibo:pmid	26209405
has license	no-cc
sha1sum (hex)	ae161c98329556419d76f0a33175e1ceae998a20
schema:url	https://doi.org/10.1016/j.jcv.2015.06.005
resource representing a document's title	Pathogen transcriptional profile in nasopharyngeal aspirates of children with acute respiratory tract infection
has PubMed Central identifier	PMC7106536
has PubMed identifier	26209405
schema:publication	J Clin Virol
resource representing a document's body	covid:ae161c98329556419d76f0a33175e1ceae998a20#body_text
is schema:about of	named entity 'Bacterial' named entity 'indicating' named entity 'Staphylococcus aureus' named entity 'tested' named entity 'viral' named entity 'RSV' named entity 'BoV' named entity 'treated' named entity 'TRANSCRIPTIONAL' named entity 'MEDIUM' named entity 'BURDEN' named entity 'RHINOVIRUS' named entity 'RSV' named entity 'INCREASE' named entity 'HUMAN METAPNEUMOVIRUS' named entity 'OBJECTIVES' named entity 'A56' named entity 'DNA VIRUS' named entity 'TESTED' named entity 'APPROACH' named entity 'PATHOGENS' named entity 'MYCOPLASMA PNEUMONIAE' named entity 'LOW' named entity 'REAL TIME PCR' named entity 'MULTIPLEX' named entity 'IMPROVED' named entity 'TECHNIQUE' named entity 'VIRAL AND' named entity 'ABSENCE OF' named entity 'TARGETS' named entity 'CONTROL' named entity 'SAMPLE' named entity 'INHIBITORS' named entity 'STUDY DESIGN' named entity 'SENSITIVE' named entity '3C10' named entity 'AGREEMENT' named entity 'PARAINFLUENZA VIRUS' named entity 'FREQUENTLY' named entity 'USE OF' named entity 'ACUTE' named entity 'RNAS' named entity 'VIRAL REPLICATION' named entity 'PEDIATRIC' named entity 'DETECTION' named entity 'CHILDREN' named entity 'NASOPHARYNGEAL' named entity 'PROFILE' named entity 'PATIENTS' named entity 'VIRUS' named entity 'ENABLED' named entity 'PATHOGEN' named entity 'SAMPLES' named entity 'LEVEL' named entity 'OUR' named entity 'BACTERIAL RNA' named entity 'STREPTOCOCCUS PNEUMONIAE' named entity 'INDICATING' named entity 'PATHOGENESIS' named entity 'INFLUENZA VIRUS' named entity 'INPUT' named entity '2886' named entity 'STRENGTHENING' named entity 'AGED' named entity 'ARI' named entity 'DETECTABLE' named entity 'BASED' named entity 'BACKGROUND' named entity 'CONFIRMED BY'

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