About: Immune responses of a designed HIV-1 DNA vaccine on rhesus monkeys

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Immune responses of a designed HIV-1 DNA vaccine on rhesus monkeys Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Immune responses of a designed HIV-1 DNA vaccine on rhesus monkeys
Creator	Wang, Hong Li, & Baoqing, Hong Dawei, Zhan Hewen, M Hongtao, Jin Ningyi, Jin Shang, Yupu Yansong, Sun Yingjin, Song Zhang, Lishu
Source	PMC
abstract	An effective HIV-1 vaccine will be the ultimate solution for the prevention of HIV/AIDS, though HAART plays important roles in treating the disease. In this study, a large-scale recombinant DNA plasmid containing a designed HIV-1 multi-epitope-p24 chimeric gene was prepared and purified. Rhesus monkeys were then inoculated muscularly with the plasmid for four times in week 0, 4, 8 and 18. Whole blood was collected two weeks after the third and fourth inoculation, followed by serum and peripheral blood mononuclear cell (PBMC) separation. The CTL activity and proliferation of PBMCs stimulated by macaque MHC-I-restricted HIV-1 CTL epitope peptide were analyzed by MTT and LDH release assay, respectively. Th1 cytokines in supernatant of cultured PBMC stimulated by HIV-1 CTL epitope peptide and anti-HIV-1 antibody in serum were assayed by ELISA. The results showed that increased CTL target-killing activity, higher secretion of Th1 cytokines (IFN-γ and IL-2) and promoted proliferative reaction of monkey PBMCs stimulated by HIV-1 CTL epitope peptide were detected in the immunization group inoculated by the recombinant DNA vaccine for three times, which were further enhanced by the fourth inoculation. At the same time, HIV-1 specific antibody in serum of immunized monkeys was higher than that in controls. We concluded that the designed HIV-1 DNA vaccine may induce HIV-1 specific cellular and humoral immunity on monkeys.
has issue date	2006-01-01(xsd:dateTime)
bibo:doi	10.1007/s11434-006-1571-9
has license	no-cc
sha1sum (hex)	ae189d61f0abc6957fa41bcd38162045ad9e9d5a
schema:url	https://doi.org/10.1007/s11434-006-1571-9
resource representing a document's title	Immune responses of a designed HIV-1 DNA vaccine on rhesus monkeys
has PubMed Central identifier	PMC7089360
schema:publication	Chin Sci Bull
resource representing a document's body	covid:ae189d61f0abc6957fa41bcd38162045ad9e9d5a#body_text
is schema:about of	named entity 'HIV-1' named entity 'inoculation' named entity 'assay' named entity 'monkeys' named entity 'activity' named entity 'antibody' named entity 'CTL' named entity 'ultimate' named entity 'serum' named entity 'PERIPHERAL BLOOD MONONUCLEAR CELL' named entity 'HIV-1' named entity 'LARGE' named entity 'DISEASE' named entity 'SOLUTION' named entity 'TARGET' named entity 'TWO WEEKS' named entity 'HIV-1 ANTIBODY' named entity 'KILLING' named entity 'LDH RELEASE ASSAY' named entity 'SERUM' named entity 'ACTIVITY' named entity 'SCALE' named entity 'ANTI-HIV' named entity 'RECOMBINANT DNA' named entity 'IMPORTANT' named entity 'SPECIFIC' named entity 'EPITOPE PEPTIDE' named entity 'FOURTH' named entity 'RESULTS' named entity 'PLASMID' named entity 'PROLIFERATIVE' named entity 'PREVENTION OF' named entity 'VACCINE' named entity 'INOCULATION' named entity 'PROLIFERATION' named entity 'TIME' named entity 'IMMUNIZATION' covid:arg/ae189d61f0abc6957fa41bcd38162045ad9e9d5a named entity 'IMMUNE RESPONSES' named entity 'RHESUS MONKEYS' named entity 'ROLES' named entity 'IL-2' named entity 'TREATING' named entity 'CONTAINING' named entity 'SEPARATION' named entity 'IMMUNIZED' named entity 'ELISA' named entity 'MACAQUE' named entity 'MULTI' named entity 'ENHANCED' named entity 'CULTURED' named entity 'STIMULATED' named entity 'TH1' named entity 'DNA VACCINE' named entity 'CHIMERIC GENE' named entity 'DETECTED' named entity 'HIV-1' named entity 'SPECIFIC ANTIBODY' named entity 'P24' named entity 'GROUP' named entity 'SECRETION' named entity 'HIV' named entity 'ANALYZED' named entity 'MTT' named entity 'CYTOKINES' named entity 'RESTRICTED' named entity 'PURIFIED' named entity 'STUDY' named entity 'SUPERNATANT' named entity 'HIGHER' named entity 'INDUCE' named entity 'WEEK' named entity 'HUMORAL IMMUNITY'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software