About: Virus susceptibility of Chinese hamster ovary (CHO) cells and detection of viral contaminations by adventitious agent testing

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About: Virus susceptibility of Chinese hamster ovary (CHO) cells and detection of viral contaminations by adventitious agent testing Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Virus susceptibility of Chinese hamster ovary (CHO) cells and detection of viral contaminations by adventitious agent testing
Creator	Kreil, Thomas Berting, Andreas Farcet, Maria
Source	Medline; PMC
abstract	Biopharmaceuticals are of increasing importance in the treatment of a variety of diseases. A remaining concern associated with their production is the potential introduction of adventitious agents into their manufacturing process, which may compromise the pathogen safety of a product and potentially cause stock‐out situations for important medical supplies. To ensure the safety of biological therapeutics, regulatory guidance requires adventitious agent testing (AAT) of the bulk harvest. AAT is a deliberately promiscuous assay procedure which has been developed to indicate, ideally, the presence of any viral contaminant. One of the most important cell lines used in the production of biopharmaceuticals is Chinese hamster ovary (CHO) cells and while viral infections of CHO cells have occurred, a systematic screen of their virus susceptibility has never been published. We investigated the susceptibility of CHO cells to infection by 14 different viruses, including members of 12 families and representatives or the very species that were implicated in previously reported production cell infections. Based on our results, four different infection outcomes were distinguished, based on the possible combinations of the two factors (i) the induction, or not, of a cytopathic effect and (ii) the ability, or not, to replicate in CHO cells. Our results demonstrate that the current AAT is effective for the detection of viruses which are able to replicate in CHO cells. Due to the restricted virus susceptibility of CHO cells and the routine AAT of bulk harvests, our results provide re‐assurance for the very high safety margins of CHO cell‐derived biopharmaceuticals. Biotechnol. Bioeng. 2010;106: 598–607. © 2010 Wiley Periodicals, Inc.
has issue date	2010-03-26(xsd:dateTime)
bibo:doi	10.1002/bit.22723
bibo:pmid	20503298
has license	no-cc
sha1sum (hex)	ae195a149ea4ae435d343f8316087f61bd337c9b
schema:url	https://doi.org/10.1002/bit.22723
resource representing a document's title	Virus susceptibility of Chinese hamster ovary (CHO) cells and detection of viral contaminations by adventitious agent testing
has PubMed Central identifier	PMC7161873
has PubMed identifier	20503298
schema:publication	Biotechnol Bioeng
resource representing a document's body	covid:ae195a149ea4ae435d343f8316087f61bd337c9b#body_text
is schema:about of	named entity 'production' named entity 'combinations' named entity 'developed' named entity 'process' named entity 'provide' named entity 'ensure' named entity 'medical' named entity 'diseases' named entity 'ARTICLE' named entity 'Virus' named entity 'based' named entity 'members' named entity 'ovary' named entity 'susceptibility' named entity 'infections' named entity 'promiscuous' named entity 'CHO cells' named entity 'viruses' named entity 'Our' named entity 'virus' named entity 'susceptibility' named entity 'CHO cells' named entity 'replicate' named entity 'adventitious agents' named entity 'CHO' named entity 'Chinese hamster ovary' named entity 'infection' named entity 'virus' named entity 'Biopharmaceuticals' named entity 'assay' named entity 'Adventitious' named entity 'cell lines' named entity 'safety' named entity 'testing' named entity '5.4' named entity 'Rabenau' named entity 'A549' named entity 'CHO' named entity 'virus' named entity 'inoculum' named entity 'Cache Valley virus' named entity 'CHO' named entity 'infection' named entity 'Reovirus' named entity 'sulfate' named entity 'CHO' named entity 'recombinant' named entity 'FDA' named entity 'supernatant' named entity 'CHO' named entity 'CHO' named entity 'CHO' named entity 'Circoviridae' named entity 'virus' named entity 'cell lines' named entity 'virus' named entity 'virus' named entity 'virus' named entity 'recombinant' named entity 'CHO' named entity 'Genzyme' named entity 'titration' named entity 'cell line' named entity 'VSV' named entity 'cell line' named entity 'Parainfluenza Virus' named entity 'amino acids' named entity 'BHK' named entity 'vaccine' named entity 'CHO' named entity 'MRC-5' named entity 'Gentamycin' named entity 'virus' named entity 'Vero' named entity 'Chinese hamster ovary' named entity 'cell culture' named entity 'virus replication' named entity 'literature survey' named entity 'Porcine circovirus'

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