About: Quantification of Eimeria necatrix, E. acervulina and E. maxima genomes in commercial chicken farms by quantitative real time PCR

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About: Quantification of Eimeria necatrix, E. acervulina and E. maxima genomes in commercial chicken farms by quantitative real time PCR Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Quantification of Eimeria necatrix, E. acervulina and E. maxima genomes in commercial chicken farms by quantitative real time PCR
Creator	Kundu, Krishnendu Banerjee, Partha Garg, • Kumar, Saroj
Source	PMC
abstract	Advent of quantitative polymerase chain reaction and its variants have enabled identification and quantification of seven known Eimeria species of poultry in biological samples. Attempts were made in the present study to identify and quantify three important pathogenic Eimeria species responsible for intestinal coccidiosis in domestic farmed chicken, E. necatrix, E. acervulina and E. maxima in droppings collected from thirty one poultry farms of North Indian states of Haryana, Punjab, Uttar Pradesh and Uttarakhand. The study included broiler, layer and backyard rearing units. Overall occurrence of E. necatrix, E. maxima and E. acervulina was 64.5%. E. necatrix was detected in 55% (11/20) broiler farms, 66.7% (4/6) layer farms and 100% (5/5) backyard rearing units studied. Thus, occurrence of E. necatrix was detected in 64.5% (20/31) farms studied. E. maxima and E. acervulina were detected in droppings of 65% (13/20) broiler farms, 66.7% (4/6) layer farms and 60% (3/5) back yard rearing units. Genome counts of each Eimeria species revealed maximum parasite load of E. necatrix followed by E. acervulina in broiler farms and least in layer farms. The mean parasite load (genome) copies for these parasite species were intermediate for backyard units while E. maxima had the lowest number of genome copies in droppings. Mean E. maxima counts were highest in boiler farms, while it was similar for layer and back yard units. However, statistically no significant differences were observed for parasite load existing either between the broiler, layer or back yard units or between the genome counts of E. necatrix, E. acervulina or E. maxima.
has issue date	2020-01-29(xsd:dateTime)
bibo:doi	10.1007/s12639-019-01188-2
has license	no-cc
sha1sum (hex)	ae45a79ede6d3509cf36403e23896c1f3ac38ecc
schema:url	https://doi.org/10.1007/s12639-019-01188-2
resource representing a document's title	Quantification of Eimeria necatrix, E. acervulina and E. maxima genomes in commercial chicken farms by quantitative real time PCR
has PubMed Central identifier	PMC7223584
schema:publication	J Parasit Dis
resource representing a document's body	covid:ae45a79ede6d3509cf36403e23896c1f3ac38ecc#body_text
is schema:about of	named entity 'polymerase' named entity 'maxima' named entity 'farms' named entity 'farms' named entity 'identify' named entity 'broiler' named entity 'Eimeria' named entity 'layer' named entity 'parasite' named entity 'units' named entity 'units' named entity 'DETECTED' named entity 'FARMS' named entity 'INCLUDED' named entity 'NORTH' named entity 'BROILER' named entity 'IMPORTANT' named entity 'DROPPINGS' named entity 'PATHOGENIC' named entity 'STATES' named entity 'UNITS' named entity 'BACK YARD' named entity 'VARIANTS' named entity 'THESE' named entity 'BOILER' named entity 'BIOLOGICAL' named entity 'CHICKEN' named entity 'HAVE' named entity 'SPECIES' named entity 'KNOWN' named entity 'LOWEST' named entity 'units' named entity 'Mean' named entity 'farms' named entity 'Overall' named entity 'number' named entity 'coccidiosis' named entity 'detected' named entity 'detected' named entity 'responsible' named entity 'layer' named entity 'chicken' named entity 'quantitative polymerase chain reaction' named entity 'Eimeria' named entity 'Eimeria' named entity 'broiler' named entity 'parasite' named entity 'Eimeria' named entity 'North Indian' named entity 'broiler' named entity 'layer' named entity 'thermal cycler' named entity 'Recombinant' named entity 'plasmid' named entity 'ITS-1' named entity 'coccidiosis' named entity 'IBM SPSS Statistics' named entity 'oocyst' named entity 'broiler' named entity 'risk factors' named entity 'disease outbreaks' named entity 'oocyst' named entity 'North India' named entity 'Uttar Pradesh' named entity 'qPCR' named entity 'statistically significant' named entity 'plasmids' named entity 'broiler' named entity 'coccidiosis' named entity 'linear regression' named entity 'recombinant vector' named entity 'broiler chicken' named entity 'primers' named entity 'Analysis of variance' named entity 'morbidity' named entity 'Uttarakhand' named entity 'oocyst' named entity 'dye' named entity 'plasmids' named entity 'oocysts'

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